Cullin 3 (Xenopus)

• Plasmid: pET28a 6His-TEV Xenopus l. Cullin 3 1-381 I320R L346D
• Parent Plasmid: pET28a original
• Expressed: 6His-TEV Xenopus l. Cullin 3 1-381 I320R L346D
• Genbank: NP_001086652.1
• Tag: 6His
• Species: Xenopus laevis

Sequence of Insert

TCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACCATGGGCTCCAGCCACCACCATCATCACCACGAAAACCTGTACTTCCAGGGCGGCTCCATGTCCAACCTGGGTAAGTCTACCGGCTCTCGTAAAGACACTAAAATGCGCATCCGTGCATTCCCGATGACTATGGACGAAAAATACGTAAACTCCATCTGGGATCTGCTGAAGAACGCGATCCAGGAAATCCAGCGCAAAAACAATAGCGGTCTGTCTTTCGAGGAACTGTACCGTAACGCTTACACCATGGTACTGCATAAACACGGTGAGAAGCTGTACACTGGTCTGCGTGAGGTGGTGACCGAGCACCTGATCAATAAGGTTCGTGAAGATGTCCTGAACAGCCTGAATAACAACTTTCTGCAGACCCTGAACCAGGCCTGGAACGACCACCAAACTGCCATGGTGATGATCCGTGACATCCTGATGTACATGGATCGTGTGTATGTCCAGCAGAATAACGTGGAAAACGTTTACAACCTGGGTCTGATCATTTTCCGTGACCAGGTAGTCCGTTATGGTTGCATCCGTGACCATCTGCGCCAGACGCTGCTGGACATGATCGCTCGTGAACGTAAAGGCGAAGTTGTCGACCGTGGTGCGATCCGTAACGCGTGTCAGATGCTGATGATCCTGGGTCTGGAGGGCCGCTCCGTGTACGAAGAAGATTTCGAGGCGCCATTCCTGGAAATGTCCGCGGAATTTTTCCAGATGGAATCCCAAAAATTCCTGGCTGAGAACAGCGCCTCCGTGTACATTAAAAAGGTGGAAGCGCGCATCAACGAAGAAATTGAACGTGTTATGCACTGCCTGGATAAATCCACGGAAGAGCCGATCGTGAAAGTGGTGGAACGTGAACTGATCTCCAAACACATGAAAACGATTGTGGAAATGGAAAACTCCGGCCTGGTACACATGCTGAAGAACGGCAAAACTGAAGACCTGGCTTGTATGTATAAACTGTTCAGCCGCGTACCGAACGGTCTGAAAACCATGTGTGAGTGTATGTCTCTGTACCTGCGTGAACAGGGCAAAGCCCTGGTGTCTGAAGAAGGCGAGGGCAAAAATCCAGTTGATTACCGTCAGGGCCTGGATGACCTGAAATCCCGCTTTGATCGCTTTCTGCAAGAAAGCTTTTCCAACGACCGTCTGTTCAAGCAGACCATCGCGGGTGACTTCGAGTACTTCCTGAACCTGAACTAAGAATTC

Amino Acid Sequence

MGSSHHHHHHENLYFQGGSMSNLGKSTGSRKDTKMRIRAFPMTMDEKYVN
SIWDLLKNAIQEIQRKNNSGLSFEELYRNAYTMVLHKHGEKLYTGLREVV
TEHLINKVREDVLNSLNNNFLQTLNQAWNDHQTAMVMIRDILMYMDRVYV
QQNNVENVYNLGLIIFRDQVVRYGCIRDHLRQTLLDMIARERKGEVVDRG
AIRNACQMLMILGLEGRSVYEEDFEAPFLEMSAEFFQMESQKFLAENSAS
VYIKKVEARINEEIERVMHCLDKSTEEPIVKVVERELISKHMKTIVEMEN
SGLVHMLKNGKTEDLACMYKLFSRVPNGLKTMCECMSLYLREQGKALVSE
EGEGKNPVDYRQGLDDLKSRFDRFLQESFSNDRLFKQTIAGDFEYFLNLN
*

• Vector Type: Bacterial
• Antibiotic: Kan
• DU Number: DU49868
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee