HECW2

• Synonyms: NEDL2
• Plasmid: pCMV5 HA HECW2 C1540A
• Parent Plasmid: pCMV5 HA
• Expressed: HA-HECW2 C1540A
• Genbank: NM_020760.1
• Tag: HA
• Species: Human

Sequence of Insert

GCGGCCGCGGTCGACATGGCTAGTTCAGCCCGGGAGCACCTGCTTTTTGTGAGGCGTCGAAATCCCCAGATGCGGTACACATTGAGCCCAGAGAACCTCCAGAGCCTTGCCGCCCAGAGCTCCATGCCAGAGAACATGACCCTGCAGCGGGCCAACAGCGACACCGACCTGGTGACTTCTGAGAGCCGCTCCAGCTTAACTGCCAGCATGTACGAGTACACGCTGGGGCAAGCCCAGAACCTCATTATCTTCTGGGACATTAAAGAGGAGGTGGACCCCAGTGATTGGATTGGACTTTATCATATAGATGAGAATTCTCCAGCCAACTTCTGGGATTCTAAGAACAGGGGTGTGACTGGAACACAAAAAGGGCAAATTGTATGGAGAATTGAGCCTGGACCCTATTTCATGGAACCGGAGATAAAAATCTGTTTTAAATATTACCACGGCATTAGTGGAGCCCTGCGAGCCACGACCCCCTGCATCACCGTGAAGAACCCAGCTGTGATGATGGGGGCAGAAGGCATGGAGGGAGGTGCTTCAGGAAACCTGCATTCTCGAAAACTTGTTAGCTTTACATTGTCAGATCTTAGGGCAGTTGGGCTAAAGAAAGGGATGTTCTTCAATCCTGACCCTTATCTTAAGATGTCAATTCAGCCAGGAAAGAAGAGCAGTTTCCCCACCTGTGCCCACCACGGGCAGGAGAGACGGTCTACTATCATCAGTAACACCACCAATCCAATTTGGCACCGAGAGAAATATTCCTTTTTTGCACTTCTTACTGATGTCTTAGAAATTGAAATTAAAGACAAATTTGCCAAGAGCCGTCCCATCATCAAGCGTTTTCTGGGGAAACTAACCATTCCAGTCCAGAGGCTGCTGGAGCGACAAGCCATCGGTGATCAAATGCTCAGCTACAACCTTGGCAGAAGGCTCCCAGCTGACCACGTGAGTGGGTACCTCCAGTTTAAAGTGGAGGTTACGTCTTCTGTTCATGAAGATGCCTCTCCAGAAGCTGTTGGCACAATACTTGGAGTCAATTCTGTGAATGGAGACTTAGGTAGCCCTTCCGATGACGAGGACATGCCAGGGAGCCATCACGACAGCCAGGTGTGCTCTAATGGGCCAGTTTCTGAGGACAGTGCTGCCGATGGAACCCCCAAGCATTCATTCAGGACTAGCTCCACTCTGGAAATAGACACAGAGGAATTAACCTCTACGTCTTCAAGGACCTCACCTCCCAGAGGACGTCAGGATTCACTCAATGATTACTTAGATGCTATCGAACACAATGGCCACTCCAGGCCAGGGACAGCGACCTGCTCTGAGAGGTCCATGGGTGCCTCTCCGAAACTGAGGAGTAGTTTTCCCACTGACACAAGACTCAATGCCATGCTTCATATTGATTCAGATGAAGAAGATCACGAGTTCCAGCAAGACCTGGGTTACCCATCTTCCTTGGAGGAGGAGGGAGGCCTGATCATGTTTAGCAGGGCATCCAGAGCTGATGATGGAAGCCTGACATCTCAGACAAAGCTGGAGGACAACCCTGTTGAGAATGAGGAAGCCTCCACACACGAAGCTGCTTCCTTTGAGGATAAGCCAGAAAATCTTCCAGAGCTTGCAGAGAGCTCCTTACCTGCAGGCCCAGCCCCAGAGGAAGGTGAAGGCGGCCCAGAGCCTCAACCCAGTGCTGACCAGGGCAGTGCTGAACTGTGTGGCTCTCAAGAGGTAGATCAGCCCACAAGTGGCGCAGACACAGGGACTTCCGATGCATCAGGAGGAAGCCGAAGAGCCGTCAGTGAGACCGAGTCTCTCGATCAGGGCTCTGAGCCTTCCCAGGTGTCCTCTGAAACAGAACCCAGTGATCCTGCCAGGACAGAGAGTGTGAGCGAAGCCAGCACCAGGCCTGAGGGAGAGAGTGACCTGGAATGCGCTGACAGCTCCTGCAATGAGAGTGTGACCACGCAGCTGTCCTCTGTGGACACACGGTGCTCCTCTCTTGAGAGCGCACGGTTTCCTGAAACCCCAGCCTTTTCTTCTCAGGAGGAGGAAGACGGAGCCTGTGCAGCAGAGCCCACCAGCAGTGGCCCTGCCGAAGGGTCGCAGGAATCCGTGTGCACTGCTGGTTCTTTACCTGTGGTACAGGTGCCCAGTGGGGAGGATGAAGGGCCAGGGGCAGAATCGGCCACTGTACCTGACCAGGAGGAGCTGGGGGAGGTCTGGCAGCGGAGGGGGAGCCTGGAGGGAGCTGCAGCTGCTGCCGAGAGCCCACCGCAAGAAGAAGGCAGTGCTGGGGAGGCCCAAGGCACCTGTGAAGGGGCAACTGCCCAGGAGGAGGGCGCTACTGGAGGTTCTCAAGCCAACGGCCACCAGCCACTGCGATCACTACCTTCAGTGCGCCAGGATGTTAGCCGGTACCAGAGGGTGGACGAGGCTCTCCCACCAAACTGGGAGGCACGCATTGACAGCCACGGCAGGATCTTCTACGTGGATCACGTAAACAGAACCACGACGTGGCAGCGACCGACAGCTCCCCCAGCCCCGCAGGTGCTGCAGAGATCTAACTCCATACAGCAAATGGAGCAGCTGAACCGGCGATATCAGAGTATCCGCAGAACCATGACCAATGAGAGGCCTGAGGAAAATACCAATGCTATTGATGGGGCAGGGGAGGAAGCTGACTTTCACCAAGCCAGTGCAGATTTCCGACGGGAGAACATCCTGCCTCACTCTACCTCCAGATCCAGGATCACGTTGCTGTTACAGTCTCCCCCCGTGAAGTTCCTCATCAGCCCAGAGTTCTTCACCGTGCTGCATTCTAACCCTAGTGCCTACCGCATGTTTACAAACAACACGTGTTTGAAGCACATGATCACCAAAGTCCGGAGGGACACCCACCACTTTGAACGCTACCAGCATAACCGCGACCTTGTGGGATTCCTCAACATGTTCGCGAACAAACAGCTAGAGCTGCCGCGGGGATGGGAAATGAAACATGATCACCAGGGCAAGGCATTCTTTGTGGACCACAACTCCCGCACCACCACTTTCATTGATCCCCGGCTCCCACTTCAGAGCAGTAGACCCACAAGTGCGCTGGTTCATCGGCAACACCTGACAAGGCAACGCAGCCACAGTGCGGGTGAGGTAGGAGAAGATTCTCGACATGCAGGACCACCAGTTCTTCCCAGGCCATCCAGTACATTCAATACAGTCAGTAGGCCACAGTACCAGGACATGGTTCCAGTGGCTTACAATGACAAGATTGTTGCATTTCTACGTCAACCCAATATCTTTGAAATTCTACAGGAGCGTCAACCTGATCTTACCAGGAATCACTCACTCAGGGAGAAGATCCAATTTATCCGAACTGAAGGGACTCCAGGATTGGTGCGCCTTTCAAGCGATGCAGACCTTGTTATGTTGCTGAGCTTATTTGAAGAAGAGATAATGTCATATGTGCCTCCACATGCCTTACTCCACCCCAGCTACTGTCAGTCCCCACGTGGCTCTCCCGTGTCATCTCCTCAGAACTCGCCAGGTACCCAGCGTGCCAATGCCCGGGCTCCAGCCCCTTACAAGCGGGATTTCGAAGCCAAACTGAGGAACTTTTACAGGAAGTTAGAGACTAAAGGATATGGACAAGGCCCAGGGAAGTTAAAGTTAATTATCCGAAGAGATCACTTACTAGAAGATGCTTTTAATCAGATTATGGGCTACTCCAGAAAAGACCTGCAGAGAAATAAGCTATATGTCACCTTCGTTGGGGAGGAAGGGCTGGATTACAGTGGGCCTTCTAGAGAGTTTTTCTTCCTGGTATCCAGAGAACTCTTTAACCCATATTATGGCTTATTTGAATATTCAGCCAATGACACATACACAGTACAAATAAGTCCTATGTCTGCTTTTGTAGACAATCACCATGAATGGTTCCGATTCAGTGGTAGGATCCTTGGTCTTGCACTAATACACCAGTATTTGTTGGATGCCTTCTTCACACGGCCCTTTTATAAGGCTCTTCTCAGAATTCTATGTGACCTGAGTGACCTAGAATACCTTGATGAAGAGTTCCATCAGAGCCTGCAGTGGATGAAAGACAATGATATCCATGACATCCTAGACCTCACGTTCACTGTGAACGAAGAAGTATTTGGGCAGATAACTGAACGAGAATTAAAGCCAGGGGGTGCCAATATCCCAGTTACAGAGAAGAACAAGAAGGAGTACATCGAGAGGATGGTGAAGTGGAGGATTGAGAGGGGTGTTGTACAGCAAACAGAGAGCTTAGTGCGTGGCTTCTATGAGGTGGTGGATGCCAGGCTGGTATCTGTTTTTGATGCAAGAGAACTGGAATTGGTCATCGCAGGCACAGCTGAAATAGACCTAAGTGATTGGAGAAACAACACAGAATATAGAGGAGGATACCATGACAATCATATTGTAATTCGGTGGTTCTGGGCTGCAGTGGAAAGATTCAACAATGAACAACGACTAAGGTTGTTACAGTTTGTTACAGGCACATCCAGCATTCCCTATGAAGGATTTGCTTCACTCCGAGGGAGTAACGGCCCAAGAAGATTCTGTGTGGAGAAATGGGGGAAAATCACTGCTCTTCCCAGAGCGCATACAGCTTTTAACCGTCTGGATCTGCCTCCCTACCCATCCTTTTCCATGCTTTATGAAAAACTGTTGACAGCAGTTGAAGAAACCAGTACTTTTGGACTTGAGTGAGCGGCCGC

Amino Acid Sequence

MYPYDVPDYAGIPGIPGSTRAAAVDMASSAREHLLFVRRRNPQMRYTLSPENLQSLAAQSSMPENMTLQRANSDTDLVTSESRSSLTASMYEYTLGQAQNLIIFWDIKEEVDPSDWIGLYHIDENSPANFWDSKNRGVTGTQKGQIVWRIEPGPYFMEPEIKICFKYYHGISGALRATTPCITVKNPAVMMGAEGMEGGASGNLHSRKLVSFTLSDLRAVGLKKGMFFNPDPYLKMSIQPGKKSSFPTCAHHGQERRSTIISNTTNPIWHREKYSFFALLTDVLEIEIKDKFAKSRPIIKRFLGKLTIPVQRLLERQAIGDQMLSYNLGRRLPADHVSGYLQFKVEVTSSVHEDASPEAVGTILGVNSVNGDLGSPSDDEDMPGSHHDSQVCSNGPVSEDSAADGTPKHSFRTSSTLEIDTEELTSTSSRTSPPRGRQDSLNDYLDAIEHNGHSRPGTATCSERSMGASPKLRSSFPTDTRLNAMLHIDSDEEDHEFQQDLGYPSSLEEEGGLIMFSRASRADDGSLTSQTKLEDNPVENEEASTHEAASFEDKPENLPELAESSLPAGPAPEEGEGGPEPQPSADQGSAELCGSQEVDQPTSGADTGTSDASGGSRRAVSETESLDQGSEPSQVSSETEPSDPARTESVSEASTRPEGESDLECADSSCNESVTTQLSSVDTRCSSLESARFPETPAFSSQEEEDGACAAEPTSSGPAEGSQESVCTAGSLPVVQVPSGEDEGPGAESATVPDQEELGEVWQRRGSLEGAAAAAESPPQEEGSAGEAQGTCEGATAQEEGATGGSQANGHQPLRSLPSVRQDVSRYQRVDEALPPNWEARIDSHGRIFYVDHVNRTTTWQRPTAPPAPQVLQRSNSIQQMEQLNRRYQSIRRTMTNERPEENTNAIDGAGEEADFHQASADFRRENILPHSTSRSRITLLLQSPPVKFLISPEFFTVLHSNPSAYRMFTNNTCLKHMITKVRRDTHHFERYQHNRDLVGFLNMFANKQLELPRGWEMKHDHQGKAFFVDHNSRTTTFIDPRLPLQSSRPTSALVHRQHLTRQRSHSAGEVGEDSRHAGPPVLPRPSSTFNTVSRPQYQDMVPVAYNDKIVAFLRQPNIFEILQERQPDLTRNHSLREKIQFIRTEGTPGLVRLSSDADLVMLLSLFEEEIMSYVPPHALLHPSYCQSPRGSPVSSPQNSPGTQRANARAPAPYKRDFEAKLRNFYRKLETKGYGQGPGKLKLIIRRDHLLEDAFNQIMGYSRKDLQRNKLYVTFVGEEGLDYSGPSREFFFLVSRELFNPYYGLFEYSANDTYTVQISPMSAFVDNHHEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRILCDLSDLEYLDEEFHQSLQWMKDNDIHDILDLTFTVNEEVFGQITERELKPGGANIPVTEKNKKEYIERMVKWRIERGVVQQTESLVRGFYEVVDARLVSVFDARELELVIAGTAEIDLSDWRNNTEYRGGYHDNHIVIRWFWAAVERFNNEQRLRLLQFVTGTSSIPYEGFASLRGSNGPRRFCVEKWGKITALPRAHTAFNRLDLPPYPSFSMLYEKLLTAVEETSTFGLE*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU25793
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee