USP40

• Plasmid: pcDNA5D FRT/TO GFP USP40
• Parent Plasmid: pcDNA5D FRT/TO GFP
• Expressed: GFP USP40
• Genbank: XM_006712612.1 GI:578804533 with silent change t1198c
• Tag: GFP
• Species: Human

Sequence of Insert

GGATCCATGTCACTTTTTTTAAGGGTAGTATTTAGTTTCACAATGTTTGGGGACCTGTTTGAAGAGGAGTATTCCACTGTGTCTAATAATCAGTATGGAAAAGGGAAGAAATTAAAGACTAAAGCTTTGGAGCCACCTGCTCCTAGAGAATTCACCAATTTAAGCGGAATCAGAAATCAGGGTGGAACCTGTTACCTCAATTCCCTTCTTCAGACTCTTCATTTCACACCTGAATTCAGAGAAGCTCTATTTTCTCTTGGCCCAGAAGAGCTTGGTTTGTTTGAAGATAAGGATAAACCCGATGCAAAGGTTCGAATCATCCCTTTACAGTTACAGCGCTTGTTTGCTCAGCTTCTGCTCTTAGACCAGGAAGCTGCATCCACAGCAGACCTCACTGACAGCTTTGGGTGGACCAGTAATGAGGAAATGAGGCAACATGATGTGCAGGAACTGAATCGAATCCTCTTCAGCGCTTTGGAAACTTCTTTAGTTGGGACCTCCGGTCATGACCTCATCTATCGTCTGTACCATGGAACCATTGTTAACCAGATTGTTTGTAAAGAATGTAAGAACGTTAGCGAGAGGCAGGAAGACTTCTTAGATCTAACAGTAGCAGTCAAAAATGTATCCGGTTTGGAAGATGCTCTCTGGAACATGTATGTAGAAGAGGAAGTTTTTGATTGTGACAACTTGTACCACTGTGGAACTTGTGACAGGCTGGTTAAAGCAGCAAAGTCGGCCAAATTACGTAAGCTGCCTCCTTTTCTTACTGTTTCATTACTAAGATTTAATTTTGATTTTGTGAAATGCGAACGCTACAAGGAAACTAGCTGTTATACATTCCCTCTCCGGATTAATCTCAAGCCCTTTTGTGAACAGAGTGAATTGGATGACTTAGAATATATATATGACCTCTTCTCAGTTATTATACACAAAGGTGGCTGCTACGGAGGCCATTACCATGTATATATTAAAGATGTTGATCATTTGGGAAACTGGCAGTTTCAAGAGGAAAAAAGTAAACCAGATGTGAATCTGAAAGATCTCCAGAGTGAAGAAGAGATTGATCATCCACTGATGATTCTAAAAGCAATCTTATTAGAGGAGGAGAATAATCTAATTCCTGTTGATCAGCTGGGCCAGAAACTTTTGAAAAAGATAGGAATATCTTGGAACAAGAAGTACAGAAAACAGCATGGACCATTGCGGAAGTTCTTACAGCTCCATTCTCAGATATTTCTACTCAGTTCAGATGAAAGTACAGTTCGTCTCTTGAAGAATAGTTCTCTCCAGGCTGAGTCTGATTTCCAAAGGAATGACCAGCAAATTTTCAAGATGCTTCCTCCAGAATCCCCAGGTTTAAACAATAGCATCTCCTGTCCCCACTGGTTTGATATAAATGATTCTAAAGTCCAGCCAATCAGGGAAAAGGATATTGAACAGCAATTTCAGGGTAAAGAAAGTGCCTACATGTTGTTTTATCGGAAATCCCAGTTGCAGAGACCCCCTGAAGCTCGAGCTAATCCAAGATATGGGGTTCCATGTCATTTACTGAATGAAATGGATGCAGCTAACATTGAACTGCAAACCAAAAGGGCAGAATGTGATTCTGCAAACAATACTTTTGAATTGCATCTTCACCTGGGCCCTCAGTATCATTTCTTCAATGGGGCTCTGCACCCAGTAGTCTCTCAAACAGAAAGCGTGTGGGATTTGACCTTTGATAAAAGAAAAACTTTAGGAGATCTCCGGCAGTCAATATTTCAGCTGTTAGAATTTTGGGAAGGAGACATGGTTCTTAGTGTTGCAAAGCTTGTACCAGCAGGACTTCACATTTACCAGTCACTTGGCGGGGATGAACTGACACTGTGTGAAACTGAAATTGCTGATGGGGAAGACATCTTTGTGTGGAATGGGGTGGAGGTTGGTGGAGTCCACATTCAAACTGGTATTGACTGCGAACCTCTACTTTTAAATGTTCTTCATCTAGACACAAGCAGTGATGGAGAAAAGTGTTGTCAGGTGATAGAATCTCCACATGTCTTTCCAGCTAATGCAGAAGTGGGCACTGTCCTCACAGCCTTAGCAATCCCAGCAGGTGTCATCTTCATCAACAGTGCTGGATGTCCAGGTGGGGAGGGTTGGACGGCCATCCCCAAGGAAGACATGAGGAAGACGTTCAGGGAGCAAGGGCTCAGAAATGGAAGCTCAATTTTAATTCAGGATTCTCATGATGATAACAGCTTGTTGACCAAGGAAGAGAAATGGGTCACTAGTATGAATGAGATTGACTGGCTCCACGTTAAAAATTTATGCCAGTTAGAATCTGAAGAGAAGCAAGTTAAAATATCAGCAACTGTTAACACAATGGTGTTTGATATTCGAATTAAAGCCATAAAGGAATTAAAATTAATGAAGGAACTAGCTGACAACAGCTGTTTGAGACCTATTGATAGAAATGGGAAGCTTCTTTGTCCAGTGCCGGACAGCTATACTTTGAAGGAAGCAGAATTGAAGATGGGAAGTTCATTGGGACTGTGTCTTGGAAAAGCACCAAGTTCGTCTCAGTTGTTCCTGTTTTTTGCAATGGGGAGTGACGTTCAACCTGGGACAGAAATGGAAATCGTAGTAGAAGAAACAATATCTGTGAGAGATTGTTTAAAGTTAATGCTGAAGAAATCTGGCCTACAAGGAGATGCCTGGCATTTACGAAAAATGGATTGGTGCTATGAAGCTGGAGAGCCTTTATGTGAAGAAGATGCAACACTGAAAGAACTTCTGATATGTTCTGGAGATACTTTGCTTTTAATTGAAGGACAACTTCCTCCTCTGGGTTTCCTGAAGGTGCCCATCTGGTGGTACCAGCTTCAGGGTCCCTCAGGACACTGGGAGAGTCATCAGGACCAGACCAACTGTACTTCGTCTTGGGGCAGAGTTTGGAGAGCCACTTCCAGCCAAGGTGCTTCTGGGAACGAGCCTGCGCAAGTTTCTCTCCTCTACTTGGGAGACATAGAGATCTCAGAAGATGCCACGCTGGCGGAGCTGAAGTCTCAGGCCATGACCTTGCCTCCTTTCCTGGAGTTCGGTGTCCCGTCCCCAGCCCACCTCAGAGCCTGGACGGTGGAGAGGAAGCGCCCAGGCAGGCTTTTACGAACTGACCGGCAGCCACTCAGGGAATATAAACTAGGACGGAGAATTGAGATCTGCTTAGAGCCCCTTCAGAAAGGCGAAAACTTGGGCCCCCAGGACGTGCTGCTGAGGACACAGGTGCGCATCCCTGGTGAGAGGACCTATGCCCCTGCCCTGGACCTGGTGTGGAACGCGGCCCAGGGTGGGACTGCCGGCTCCCTGAGGCAGAGAGTTGCCGATTTCTATCGTCTTCCCGTGGAGAAGATTGAAATTGCCAAATACTTTCCCGAAAAGTTCGAGTGGCTTCCGATATCTAGCTGGAACCAACAAATAACCAAGAGGAAAAAGAAAAAAAAACAAGATTATTTGCAAGGGGCACCGTATTACTTGAAAGACGGAGATACTATTGGTGTTAAGAATCTCCTGATTGACGACGATGATGATTTCAGTACAATCAGAGATGACACTGGAAAAGAAAAGCAGAAACAACGGGCCCTGGGGAGAAGGAAAAGCCAAGAAGCCCTCCATGAGCAGAGCAGCTACATCCTCTCCAGTGCAGAGACGCCTGCCCGGCCCCGAGCCCCGGAAACTTCTCTCTCCATCCACGTGGGGAGCTTCAGATAAGCGGCCGC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSMSLFLR
VVFSFTMFGDLFEEEYSTVSNNQYGKGKKLKTKALEPPAPREFTNLSGIR
NQGGTCYLNSLLQTLHFTPEFREALFSLGPEELGLFEDKDKPDAKVRIIP
LQLQRLFAQLLLLDQEAASTADLTDSFGWTSNEEMRQHDVQELNRILFSA
LETSLVGTSGHDLIYRLYHGTIVNQIVCKECKNVSERQEDFLDLTVAVKN
VSGLEDALWNMYVEEEVFDCDNLYHCGTCDRLVKAAKSAKLRKLPPFLTV
SLLRFNFDFVKCERYKETSCYTFPLRINLKPFCEQSELDDLEYIYDLFSV
IIHKGGCYGGHYHVYIKDVDHLGNWQFQEEKSKPDVNLKDLQSEEEIDHP
LMILKAILLEEENNLIPVDQLGQKLLKKIGISWNKKYRKQHGPLRKFLQL
HSQIFLLSSDESTVRLLKNSSLQAESDFQRNDQQIFKMLPPESPGLNNSI
SCPHWFDINDSKVQPIREKDIEQQFQGKESAYMLFYRKSQLQRPPEARAN
PRYGVPCHLLNEMDAANIELQTKRAECDSANNTFELHLHLGPQYHFFNGA
LHPVVSQTESVWDLTFDKRKTLGDLRQSIFQLLEFWEGDMVLSVAKLVPA
GLHIYQSLGGDELTLCETEIADGEDIFVWNGVEVGGVHIQTGIDCEPLLL
NVLHLDTSSDGEKCCQVIESPHVFPANAEVGTVLTALAIPAGVIFINSAG
CPGGEGWTAIPKEDMRKTFREQGLRNGSSILIQDSHDDNSLLTKEEKWVT
SMNEIDWLHVKNLCQLESEEKQVKISATVNTMVFDIRIKAIKELKLMKEL
ADNSCLRPIDRNGKLLCPVPDSYTLKEAELKMGSSLGLCLGKAPSSSQLF
LFFAMGSDVQPGTEMEIVVEETISVRDCLKLMLKKSGLQGDAWHLRKMDW
CYEAGEPLCEEDATLKELLICSGDTLLLIEGQLPPLGFLKVPIWWYQLQG
PSGHWESHQDQTNCTSSWGRVWRATSSQGASGNEPAQVSLLYLGDIEISE
DATLAELKSQAMTLPPFLEFGVPSPAHLRAWTVERKRPGRLLRTDRQPLR
EYKLGRRIEICLEPLQKGENLGPQDVLLRTQVRIPGERTYAPALDLVWNA
AQGGTAGSLRQRVADFYRLPVEKIEIAKYFPEKFEWLPISSWNQQITKRK
KKKKQDYLQGAPYYLKDGDTIGVKNLLIDDDDDFSTIRDDTGKEKQKQRA
LGRRKSQEALHEQSSYILSSAETPARPRAPETSLSIHVGSFR*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU49677
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee