USP54

• Synonyms: bA137L10.3; bA137L10.4; C10orf29
• Plasmid: pcDNA5 FRT/TO GFP.DU USP54 C42A
• Parent Plasmid: pcDNA5D FRT/TO GFP
• Expressed: GFP-USP54 C42A
• Genbank: XM_005269578.1 GI:530393169 with silent change a2883g
• Tag: GFP
• Species: Human

Sequence of Insert

AGATCTATGTCTTGGAAGAGAAATTATTTTTCAGGGGGTCGTGGTAGTGTACAAGGGATGTTTGCACCTCGAAGCTCAACCTCCATAGCCCCCAGCAAAGGCCTCAGCAATGAGCCAGGGCAAAACAGCGCCTTCCTCAACAGTGCCCTGCAGGTTTTGTGGCACTTGGATATCTTCCGACGTAGCTTTAGGCAGCTTACAACTCACAAGTGCATGGGAGATTCCTGCATCTTTTGCGCTCTCAAGGGAATCTTTAACCAGTTTCAGTGTAGTAGTGAAAAAGTGCTTCCATCTGACACTCTCCGCAGTGCTCTGGCAAAGACTTTCCAGGATGAACAACGTTTCCAGCTGGGAATTATGGATGATGCTGCAGAGTGCTTTGAAAACCTCCTGATGAGAATTCACTTCCACATTGCTGATGAAACCAAAGAGGATATATGTACTGCCCAACACTGCATTTCCCATCAGAAATTTGCAATGACATTGTTTGAGCAGTGTGTATGTACTAGCTGTGGTGCCACTTCTGATCCGCTGCCTTTCATCCAGATGGTACATTATATCTCCACCACTTCCCTTTGCAATCAGGCTATTTGTATGCTGGAAAGACGAGAGAAACCTTCACCAAGCATGTTTGGTGAGCTGCTGCAGAATGCCAGCACCATGGGGGATCTGCGGAACTGTCCAAGCAACTGTGGAGAGAGGATCAGGATTCGCCGTGTGTTGATGAATGCTCCACAGATTATCACGATTGGGCTGGTATGGGACTCAGACCACTCAGACTTAGCAGAAGATGTTATCCACAGCCTGGGAACCTGCCTTAAGCTGGGTGATCTGTTTTTCAGAGTGACGGATGACCGGGCCAAGCAATCTGAACTGTACTTAGTTGGAATGATCTGTTACTATGGCAAACATTATTCTACATTCTTTTTTCAAACAAAGATTCGCAAATGGATGTATTTTGATGATGCTCATGTCAAGGAGATTGGGCCCAAATGGAAGGATGTGGTGACCAAATGCATCAAGGGGCATTATCAGCCCCTGCTGCTGCTTTATGCAGATCCCCAGGGTACCCCAGTTTCCACCCAGGACCTGCCTCCCCAAGCTGAGTTCCAGTCATACAGCAGGACATGCTACGACAGTGAAGATTCAGGGAGGGAGCCCTCCATCTCAAGTGACACTCGAACAGATTCCTCAACGGAGAGCTATCCCTACAAACATTCCCACCATGAGTCTGTGGTCAGTCACTTCTCTTCTGATTCTCAGGGGACAGTCATCTATAATGTGGAAAATGATTCCATGTCTCAGAGCAGTCGGGACACAGGACACCTGACTGATAGTGAATGTAATCAGAAACACACATCCAAGAAAGGGTCACTGATAGAGCGCAAGAGGAGCTCTGGTCGGGTTAGGAGGAAAGGCGATGAGCCCCAGGCCTCGGGATACCACAGTGAAGGAGAAACACTGAAAGAGAAGCAGGCTCCTAGAAATGCCTCCAAACCATCCAGCAGCACCAACAGGCTGAGAGATTTTAAAGAGACAGTCAGCAATATGATCCATAACAGACCATCCCTGGCTTCTCAGACCAATGTAGGCTCTCACTGCAGGGGCAGAGGAGGAGACCAGCCTGACAAAAAACCTCCTAGGACCCTGCCTTTACACTCTCGTGACTGGGAAATAGAGAGTACCAGCAGTGAGTCAAAATCCAGTTCTTCCAGCAAGTATCGTCCCACATGGAGACCCAAACGAGAATCTCTGAATATTGACAGTATCTTTAGTAAGGACAAAAGGAAGCACTGTGGCTATACCCAGCTTAGCCCCTTTTCTGAGGATTCAGCTAAAGAATTTATACCAGATGAACCAAGCAAGCCACCTTCTTACGACATTAAATTTGGTGGACCAAGCCCCCAGTACAAGCGCTGGGGCCCAGCACGGCCAGGCTCTCACCTTTTAGAGCAGCACCCCCGACTAATCCAGCGAATGGAATCTGGCTATGAAAGCAGTGAGAGGAACAGCAGCAGCCCTGTCAGCCTGGATGCAGCCCTGCCTGAGAGCTCAAATGTCTACAGGGATCCAAGTGCTAAGAGATCAGCTGGGTTGGTTCCTTCCTGGCGTCATATCCCAAAGTCGCACAGCAGTAGCATCCTGGAGGTAGACTCCACAGCATCCATGGGTGGCTGGACAAAGAGTCAGCCTTTCTCTGGTGAGGAGATATCTTCTAAAAGTGAACTGGATGAATTGCAGGAAGAGGTGGCCAGGAGGGCGCAGGAACAGGAACTTCGAAGAAAACGGGAGAAGGAGTTAGAGGCAGCGAAAGGGTTTAACCCTCATCCTAGCCGCTTCATGGACTTGGATGAACTGCAGAATCAGGGGAGGAGTGACGGCTTTGAGAGGTCCCTGCAAGAGGCAGAGTCAGTGTTTGAAGAGTCACTACATCTGGAACAGAAAGGAGACTGTGCTGCAGCTTTGGCTCTCTGTAATGAAGCTATCTCTAAACTAAGACTTGCCCTGCATGGTGCCAGCTGTAGCACGCACAGCAGAGCCCTAGTCGATAAGAAGTTGCAAATCAGTATTCGAAAAGCACGGAGCCTGCAGGATCGCATGCAGCAGCAGCAATCACCACAGCAGCCGTCGCAGCCCTCAGCCTGCCTCCCAACACAGGCGGGGACTCTCTCTCAGCCAACAAGTTCCTTTGGCCGGGCTGCCTCTCAGGACCCCAGTGAACAGCCTATCCCGCTCCAAGTATTGTTAAGCCAAGAGGCCCAACTGGAATCCGGCATGGATACAGAGTTTGGGGCCAGTTCTTTCTTCCATTCACCTGCTTCCTGCCATGAGTCACACTCATCACTATCTCCAGAGTCATCTGCCCCACAGCACAGCTCCCCCAGTAGGTCTGCCTTGAAGCTTCTGACTTCGGTTGAAGTAGACAACATTGAACCCTCTGCATTCCACAGGCAAGGTTTACCTAAAGCACCAGGGTGGACTGAGAAGAATTCTCATCATAGTTGGGAGCCATTGGATGCCCCAGAGGGTAAGCTGCAAGGCTCTAGGTGTGACAACAGCAGTTGCAGCAAGCTCCCTCCACAAGAAGGAAGAGGCATTGCTCAAGAACAGCTGTTCCAAGAAAAGAAGGATCCTGCTAACCCCTCCCCGGTGATGCCTGGAATAGCCACCTCTGAGAGGGGTGATGAACACAGCCTAGGCTGTAGTCCTTCAAATTCATCAGCTCAGCCCAGCCTTCCCCTGTATAGAACCTGCCACCCCATAATGCCTGTTGCTTCTTCATTTGTGCTTCACTGTCCTGATCCTGTGCAGAAAACTAACCAATGCCTCCAAGGCCAAAGCCTCAAAACTTCATTGACTTTAAAAGTGGACAGAGGCAGTGAGGAGACCTATAGGCCAGAGTTTCCCAGCACAAAGGGGCTTGTCCGTTCTCTGGCTGAGCAGTTCCAGAGGATGCAGGGTGTCTCCATGAGGGATAGTACAGGTTTCAAGGATAGAAGTTTGTCAGGTAGTCTAAGGAAGAACTCTTCCCCTTCTGATTCTAAGCCTCCTTTCTCACAGGGTCAAGAGAAAGGCCACTGGCCATGGGCAAAGCAACAATCCTCTCTGGAGGGTGGGGATAGACCACTTTCCTGGGAAGAGTCCACTGAACATTCTTCTCTTGCCTTAAACTCTGGGCTGCCTAATGGTGAAACTTCTAGCGGAGGACAGCCCAGGTTGGCAGAGCCAGACATATACCAAGAGAAGCTGTCCCAAGTGAGAGATGTTAGGTCTAAGGATCTGGGCAGCAGTACTGACTTGGGGACTTCCTTGCCTTTGGATTCCTGGGTGAATATCACAAGGTTCTGTGATTCTCAGCTTAAGCATGGGGCACCTAGGCCAGGAATGAAGTCCTCCCCTCATGATTCCCATACGTGTGTAACCTATCCAGAGAGAAATCACATCCTTTTGCATCCACATTGGAACCAAGACACAGAGCAGGAGACCTCAGAATTGGAGTCTCTGTATCAGGCCAGTCTTCAGGCTTCTCAAGCTGGCTGTTCTGGATGGGGGCAGCAGGATACCGCCTGGCACCCACTTAGCCAAACAGGCTCTGCAGATGGCATGGGGAGGAGGTTGCACTCAGCCCATGATCCTGGTCTCTCAAAGACTTCAACAGCAGAAATGGAGCATGGTCTCCATGAAGCCAGAACAGTGCGTACTTCTCAGGCTACACCTTGCCGAGGCCTCAGCAGGGAGTGTGGGGAGGATGAGCAGTACAGTGCAGAGAATTTACGTCGCATCTCACGCAGTCTCAGTGGCACCGTTGTCTCAGAGAGGGAGGAAGCTCCGGTTTCTTCCCACAGTTTTGATTCATCAAACGTGAGGAAGCCTTTGGAAACCGGGCACCGTTGTTCCAGCTCCTCTTCCCTCCCTGTCATCCATGACCCTTCTGTGTTTCTCCTCGGTCCCCAACTCTACCTTCCCCAACCACAGTTCCTGTCCCCAGATGTCCTGATGCCCACCATGGCAGGGGAGCCCAATAGACTCCCAGGAACTTCAAGGAGTGTCCAGCAGTTTCTGGCTATGTGTGACAGGGGTGAAACTTCCCAAGGGGCCAAGTACACAGGAAGGACTTTGAACTACCAGAGCCTCCCCCATCGCTCCAGAACAGACAACTCCTGGGCACCCTGGTCAGAGACCAACCAGCATATTGGGACCAGATTCCTGACTACTCCAGGGTGCAATCCTCAACTAACCTACACTGCCACACTACCAGAAAGAAGCAAGGGCCTTCAGGTTCCTCACACTCAGTCCTGGAGTGATCTTTTCCATTCACCCTCCCACCCTCCCATTGTTCATCCTGTGTACCCACCATCTAGCAGTCTTCATGTACCCCTGAGGTCAGCTTGGAATTCAGATCCTGTTCCAGGGTCCCGAACCCCTGGTCCTCGAAGAGTAGATATGCCCCCAGATGATGACTGGAGGCAAAGCAGTTATGCCTCCCACTCTGGACACAGGAGAACAGTGGGAGAGGGGTTTCTGTTTGTTCTATCAGATGCTCCCAGAAGAGAGCAGATCAGGGCTAGAGTCCTGCAGCACAGTCAATGGTAAGCGGCCGC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSMSWKRN
YFSGGRGSVQGMFAPRSSTSIAPSKGLSNEPGQNSAFLNSALQVLWHLDI
FRRSFRQLTTHKCMGDSCIFCALKGIFNQFQCSSEKVLPSDTLRSALAKT
FQDEQRFQLGIMDDAAECFENLLMRIHFHIADETKEDICTAQHCISHQKF
AMTLFEQCVCTSCGATSDPLPFIQMVHYISTTSLCNQAICMLERREKPSP
SMFGELLQNASTMGDLRNCPSNCGERIRIRRVLMNAPQIITIGLVWDSDH
SDLAEDVIHSLGTCLKLGDLFFRVTDDRAKQSELYLVGMICYYGKHYSTF
FFQTKIRKWMYFDDAHVKEIGPKWKDVVTKCIKGHYQPLLLLYADPQGTP
VSTQDLPPQAEFQSYSRTCYDSEDSGREPSISSDTRTDSSTESYPYKHSH
HESVVSHFSSDSQGTVIYNVENDSMSQSSRDTGHLTDSECNQKHTSKKGS
LIERKRSSGRVRRKGDEPQASGYHSEGETLKEKQAPRNASKPSSSTNRLR
DFKETVSNMIHNRPSLASQTNVGSHCRGRGGDQPDKKPPRTLPLHSRDWE
IESTSSESKSSSSSKYRPTWRPKRESLNIDSIFSKDKRKHCGYTQLSPFS
EDSAKEFIPDEPSKPPSYDIKFGGPSPQYKRWGPARPGSHLLEQHPRLIQ
RMESGYESSERNSSSPVSLDAALPESSNVYRDPSAKRSAGLVPSWRHIPK
SHSSSILEVDSTASMGGWTKSQPFSGEEISSKSELDELQEEVARRAQEQE
LRRKREKELEAAKGFNPHPSRFMDLDELQNQGRSDGFERSLQEAESVFEE
SLHLEQKGDCAAALALCNEAISKLRLALHGASCSTHSRALVDKKLQISIR
KARSLQDRMQQQQSPQQPSQPSACLPTQAGTLSQPTSSFGRAASQDPSEQ
PIPLQVLLSQEAQLESGMDTEFGASSFFHSPASCHESHSSLSPESSAPQH
SSPSRSALKLLTSVEVDNIEPSAFHRQGLPKAPGWTEKNSHHSWEPLDAP
EGKLQGSRCDNSSCSKLPPQEGRGIAQEQLFQEKKDPANPSPVMPGIATS
ERGDEHSLGCSPSNSSAQPSLPLYRTCHPIMPVASSFVLHCPDPVQKTNQ
CLQGQSLKTSLTLKVDRGSEETYRPEFPSTKGLVRSLAEQFQRMQGVSMR
DSTGFKDRSLSGSLRKNSSPSDSKPPFSQGQEKGHWPWAKQQSSLEGGDR
PLSWEESTEHSSLALNSGLPNGETSSGGQPRLAEPDIYQEKLSQVRDVRS
KDLGSSTDLGTSLPLDSWVNITRFCDSQLKHGAPRPGMKSSPHDSHTCVT
YPERNHILLHPHWNQDTEQETSELESLYQASLQASQAGCSGWGQQDTAWH
PLSQTGSADGMGRRLHSAHDPGLSKTSTAEMEHGLHEARTVRTSQATPCR
GLSRECGEDEQYSAENLRRISRSLSGTVVSEREEAPVSSHSFDSSNVRKP
LETGHRCSSSSSLPVIHDPSVFLLGPQLYLPQPQFLSPDVLMPTMAGEPN
RLPGTSRSVQQFLAMCDRGETSQGAKYTGRTLNYQSLPHRSRTDNSWAPW
SETNQHIGTRFLTTPGCNPQLTYTATLPERSKGLQVPHTQSWSDLFHSPS
HPPIVHPVYPPSSSLHVPLRSAWNSDPVPGSRTPGPRRVDMPPDDDWRQS
SYASHSGHRRTVGEGFLFVLSDAPRREQIRARVLQHSQW*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU49118
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee