USP36

• Synonyms: DUB1
• Plasmid: pcDNA5 FRT/TO USP36-GFP
• Parent Plasmid: pcDNA5 FRT/TO C-GFP
• Expressed: USP36-GFP
• Genbank: NM_025090.3 GI:122114650 with silent change c1863t
• Tag: GFP
• Species: Human

Sequence of Insert

GGATCCATGCCAATAGTGGATAAGTTGAAGGAGGCCCTGAAACCCGGCCGCAAGGACTCGGCTGATGATGGAGAACTGGGGAAGCTTCTTGCCTCCTCTGCCAAGAAGGTCCTTTTACAGAAAATCGAGTTCGAGCCAGCCAGCAAGAGCTTCTCCTACCAGCTGGAGGCCTTAAAGAGCAAATATGTGTTGCTCAACCCCAAAACAGAGGGAGCTAGTCGCCACAAGAGTGGAGATGACCCACCGGCCAGGAGACAGGGCAGTGAGCACACGTATGAGAGCTGTGGTGACGGAGTCCCAGCCCCGCAGAAAGTGCTTTTCCCCACGGAGCGACTGTCTCTGAGGTGGGAGCGGGTCTTCCGCGTGGGCGCAGGACTCCACAACCTTGGCAACACCTGCTTTCTCAATGCCACCATCCAGTGCTTGACCTACACACCACCTCTAGCCAACTACCTGCTCTCCAAGGAGCATGCTCGCAGCTGCCACCAGGGAAGCTTCTGCATGCTGTGTGTCATGCAGAACCACATTGTCCAGGCCTTCGCCAACAGCGGCAACGCCATCAAGCCCGTCTCCTTCATCCGAGACCTGAAAAAGATCGCCCGACACTTCCGCTTTGGGAACCAGGAGGACGCGCATGAGTTCCTGCGGTACACCATCGACGCCATGCAGAAAGCCTGCCTGAATGGCTGTGCCAAGTTGGATCGTCAAACGCAGGCTACTACCTTGGTCCATCAAATTTTTGGAGGGTATCTCAGATCACGCGTGAAGTGCTCCGTGTGCAAGAGCGTCTCGGACACCTACGACCCCTACTTGGACGTCGCGCTGGAGATCCGGCAAGCTGCGAATATTGTGCGTGCTCTGGAACTTTTTGTGAAAGCAGATGTCCTGAGTGGAGAGAATGCCTACATGTGTGCTAAATGCAAGAAGAAGGTTCCAGCCAGCAAGCGCTTCACCATCCACAGAACATCCAACGTCTTAACCCTTTCCCTCAAGCGCTTTGCCAACTTCAGCGGGGGGAAGATCACCAAGGATGTAGGCTATCCGGAATTCCTCAACATACGTCCGTATATGTCCCAGAATAATGGTGATCCTGTCATGTATGGACTCTATGCTGTCCTGGTGCACTCGGGCTACAGCTGCCATGCCGGGCACTATTACTGCTACGTGAAGGCAAGCAATGGACAGTGGTACCAGATGAATGATTCCTTGGTCCATTCCAGCAACGTCAAGGTGGTTCTGAACCAGCAGGCCTACGTGCTGTTCTATCTGCGAATTCCAGGCTCTAAGAAAAGTCCCGAGGGCCTCATCTCCAGGACAGGCTCCTCCTCCCTTCCCGGCCGCCCGAGTGTGATTCCAGATCACTCCAAGAAGAACATCGGCAATGGGATTATTTCCTCCCCACTGACTGGAAAGCGACAAGACTCTGGGACGATGAAGAAGCCGCACACCACTGAAGAGATTGGTGTGCCCATATCCAGGAATGGCTCCACCCTGGGCCTGAAGTCCCAGAACGGCTGCATTCCTCCAAAGCTGCCCTCGGGGTCCCCTTCCCCCAAACTCTCCCAGACACCCACACACATGCCAACCATCCTAGACGACCCTGGAAAGAAGGTGAAGAAGCCAGCTCCTCCACAGCACTTTTCCCCCAGAACTGCTCAGGGGCTGCCTGGGACCAGCAACTCGAATAGCAGCAGATCTGGGAGCCAAAGGCAGGGCTCCTGGGACAGCAGGGATGTTGTCCTCTCTACCTCACCTAAGCTCCTGGCTACAGCCACTGCCAACGGGCATGGGCTGAAGGGGAACGACGAGAGCGCTGGCCTCGACAGGAGGGGCTCCAGCAGCTCCAGCCCAGAGCACTCGGCCAGCAGTGACTCCACCAAGGCCCCCCAGACCCCCAGGAGTGGAGCGGCCCATCTCTGCGATTCTCAGGAAACGAACTGTTCCACCGCTGGCCACTCCAAAACGCCGCCAAGTGGAGCAGATTCTAAGACGGTGAAGCTGAAGTCCCCTGTCCTGAGCAACACCACCACTGAGCCTGCAAGCACCATGTCTCCTCCACCAGCCAAAAAACTGGCCCTTTCTGCCAAGAAGGCCAGCACCCTGTGGAGGGCGACCGGCAATGACCTCCGTCCACCTCCCCCCTCACCATCCTCCGACCTCACCCACCCCATGAAAACCTCTCACCCCGTCGTTGCCTCCACTTGGCCCGTCCATAGAGCCAGGGCTGTGTCACCTGCTCCCCAATCATCCAGCCGCCTGCAACCCCCCTTCAGCCCCCACCCCACATTGCTGTCCAGTACCCCCAAGCCCCCAGGGACGTCAGAACCACGGAGCTGCTCCTCCATCTCGACGGCGCTGCCTCAGGTCAACGAGGACCTTGTGTCTCTTCCACACCAGTTGCCAGAGGCCAGTGAGCCCCCCCAGAGCCCCTCTGAGAAGAGGAAAAAGACCTTTGTGGGAGAGCCGCAGAGGCTGGGCTCAGAGACGCGCCTCCCACAGCACATCAGGGAGGCCACTGCGGCTCCCCACGGGAAGAGGAAGAGGAAGAAGAAGAAGCGCCCGGAGGACACAGCTGCCAGCGCCCTGCAGGAGGGGCAGACACAGAGACAGCCTGGGAGCCCCATGTACAGGAGGGAGGGCCAGGCACAGCTGCCCGCTGTCAGACGGCAGGAAGATGGCACACAGCCACAGGTGAATGGCCAGCAGGTGGGATGTGTTACGGACGGCCACCACGCGAGCAGCAGGAAGCGGAGGAGGAAAGGAGCAGAAGGTCTTGGTGAAGAAGGCGGCCTGCACCAGGACCCACTTCGGCACAGCTGCTCTCCCATGGGTGATGGTGATCCAGAGGCCATGGAAGAGTCTCCAAGGAAAAAGAAAAAGAAAAAAAGAAAGCAGGAGACACAGCGGGCAGTAGAAGAGGATGGGCATCTCAAATGCCCAAGGAGTGCCAAGCCCCAAGATGCTGTTGTCCCCGAGTCCAGCAGCTGCGCACCATCCGCGAATGGCTGGTGTCCTGGGGACCGCATGGGGCTGAGCCAGGCCCCTCCTGTGTCTTGGAATGGAGAGCGGGAGTCTGATGTGGTCCAGGAACTGCTCAAATACTCATCTGATAAAGCTTACGGGAGAAAAGTTCTGACCTGGGATGGCAAGATGTCGGCGGTCAGTCAGGATGCTATTGAAGACAGCAGACAGGCCCGGACTGAGACCGTGGTTGATGACTGGGACGAAGAGTTTGACCGAGGGAAGGAAAAGAAAATTAAAAAATTTAAGAGAGAGAAGAGGAGAAACTTCAACGCCTTCCAGAAACTTCAGACTCGACGGAACTTCTGGTCTGTGACTCACCCAGCAAAGGCTGCCAGCCTCAGCTATCGCCGCGCGGCCGC

Amino Acid Sequence

MPIVDKLKEALKPGRKDSADDGELGKLLASSAKKVLLQKIEFEPASKSFS
YQLEALKSKYVLLNPKTEGASRHKSGDDPPARRQGSEHTYESCGDGVPAP
QKVLFPTERLSLRWERVFRVGAGLHNLGNTCFLNATIQCLTYTPPLANYL
LSKEHARSCHQGSFCMLCVMQNHIVQAFANSGNAIKPVSFIRDLKKIARH
FRFGNQEDAHEFLRYTIDAMQKACLNGCAKLDRQTQATTLVHQIFGGYLR
SRVKCSVCKSVSDTYDPYLDVALEIRQAANIVRALELFVKADVLSGENAY
MCAKCKKKVPASKRFTIHRTSNVLTLSLKRFANFSGGKITKDVGYPEFLN
IRPYMSQNNGDPVMYGLYAVLVHSGYSCHAGHYYCYVKASNGQWYQMNDS
LVHSSNVKVVLNQQAYVLFYLRIPGSKKSPEGLISRTGSSSLPGRPSVIP
DHSKKNIGNGIISSPLTGKRQDSGTMKKPHTTEEIGVPISRNGSTLGLKS
QNGCIPPKLPSGSPSPKLSQTPTHMPTILDDPGKKVKKPAPPQHFSPRTA
QGLPGTSNSNSSRSGSQRQGSWDSRDVVLSTSPKLLATATANGHGLKGND
ESAGLDRRGSSSSSPEHSASSDSTKAPQTPRSGAAHLCDSQETNCSTAGH
SKTPPSGADSKTVKLKSPVLSNTTTEPASTMSPPPAKKLALSAKKASTLW
RATGNDLRPPPPSPSSDLTHPMKTSHPVVASTWPVHRARAVSPAPQSSSR
LQPPFSPHPTLLSSTPKPPGTSEPRSCSSISTALPQVNEDLVSLPHQLPE
ASEPPQSPSEKRKKTFVGEPQRLGSETRLPQHIREATAAPHGKRKRKKKK
RPEDTAASALQEGQTQRQPGSPMYRREGQAQLPAVRRQEDGTQPQVNGQQ
VGCVTDGHHASSRKRRRKGAEGLGEEGGLHQDPLRHSCSPMGDGDPEAME
ESPRKKKKKKRKQETQRAVEEDGHLKCPRSAKPQDAVVPESSSCAPSANG
WCPGDRMGLSQAPPVSWNGERESDVVQELLKYSSDKAYGRKVLTWDGKMS
AVSQDAIEDSRQARTETVVDDWDEEFDRGKEKKIKKFKREKRRNFNAFQK
LQTRRNFWSVTHPAKAASLSYRRAAAMVSKGEELFTGVVPILVELDGDVN
GHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSR
YPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRI
ELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIED
GSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
VTAAGITLGMDELYK*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU49062
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee