USP54

• Synonyms: bA137L10.3; bA137L10.4; C10orf29
• Plasmid: pcDNA5 FRT/TO USP54-GFP
• Parent Plasmid: pcDNA5D FRT/TO C-GFP
• Expressed: USP54-GFP
• Genbank: XM_005269578.1 GI:530393169 with silent change a2883g
• Tag: GFP
• Species: Human

Sequence of Insert

AGATCTGCCACCATGTCTTGGAAGAGAAATTATTTTTCAGGGGGTCGTGGTAGTGTACAAGGGATGTTTGCACCTCGAAGCTCAACCTCCATAGCCCCCAGCAAAGGCCTCAGCAATGAGCCAGGGCAAAACAGCTGCTTCCTCAACAGTGCCCTGCAGGTTTTGTGGCACTTGGATATCTTCCGACGTAGCTTTAGGCAGCTTACAACTCACAAGTGCATGGGAGATTCCTGCATCTTTTGCGCTCTCAAGGGAATCTTTAACCAGTTTCAGTGTAGTAGTGAAAAAGTGCTTCCATCTGACACTCTCCGCAGTGCTCTGGCAAAGACTTTCCAGGATGAACAACGTTTCCAGCTGGGAATTATGGATGATGCTGCAGAGTGCTTTGAAAACCTCCTGATGAGAATTCACTTCCACATTGCTGATGAAACCAAAGAGGATATATGTACTGCCCAACACTGCATTTCCCATCAGAAATTTGCAATGACATTGTTTGAGCAGTGTGTATGTACTAGCTGTGGTGCCACTTCTGATCCGCTGCCTTTCATCCAGATGGTACATTATATCTCCACCACTTCCCTTTGCAATCAGGCTATTTGTATGCTGGAAAGACGAGAGAAACCTTCACCAAGCATGTTTGGTGAGCTGCTGCAGAATGCCAGCACCATGGGGGATCTGCGGAACTGTCCAAGCAACTGTGGAGAGAGGATCAGGATTCGCCGTGTGTTGATGAATGCTCCACAGATTATCACGATTGGGCTGGTATGGGACTCAGACCACTCAGACTTAGCAGAAGATGTTATCCACAGCCTGGGAACCTGCCTTAAGCTGGGTGATCTGTTTTTCAGAGTGACGGATGACCGGGCCAAGCAATCTGAACTGTACTTAGTTGGAATGATCTGTTACTATGGCAAACATTATTCTACATTCTTTTTTCAAACAAAGATTCGCAAATGGATGTATTTTGATGATGCTCATGTCAAGGAGATTGGGCCCAAATGGAAGGATGTGGTGACCAAATGCATCAAGGGGCATTATCAGCCCCTGCTGCTGCTTTATGCAGATCCCCAGGGTACCCCAGTTTCCACCCAGGACCTGCCTCCCCAAGCTGAGTTCCAGTCATACAGCAGGACATGCTACGACAGTGAAGATTCAGGGAGGGAGCCCTCCATCTCAAGTGACACTCGAACAGATTCCTCAACGGAGAGCTATCCCTACAAACATTCCCACCATGAGTCTGTGGTCAGTCACTTCTCTTCTGATTCTCAGGGGACAGTCATCTATAATGTGGAAAATGATTCCATGTCTCAGAGCAGTCGGGACACAGGACACCTGACTGATAGTGAATGTAATCAGAAACACACATCCAAGAAAGGGTCACTGATAGAGCGCAAGAGGAGCTCTGGTCGGGTTAGGAGGAAAGGCGATGAGCCCCAGGCCTCGGGATACCACAGTGAAGGAGAAACACTGAAAGAGAAGCAGGCTCCTAGAAATGCCTCCAAACCATCCAGCAGCACCAACAGGCTGAGAGATTTTAAAGAGACAGTCAGCAATATGATCCATAACAGACCATCCCTGGCTTCTCAGACCAATGTAGGCTCTCACTGCAGGGGCAGAGGAGGAGACCAGCCTGACAAAAAACCTCCTAGGACCCTGCCTTTACACTCTCGTGACTGGGAAATAGAGAGTACCAGCAGTGAGTCAAAATCCAGTTCTTCCAGCAAGTATCGTCCCACATGGAGACCCAAACGAGAATCTCTGAATATTGACAGTATCTTTAGTAAGGACAAAAGGAAGCACTGTGGCTATACCCAGCTTAGCCCCTTTTCTGAGGATTCAGCTAAAGAATTTATACCAGATGAACCAAGCAAGCCACCTTCTTACGACATTAAATTTGGTGGACCAAGCCCCCAGTACAAGCGCTGGGGCCCAGCACGGCCAGGCTCTCACCTTTTAGAGCAGCACCCCCGACTAATCCAGCGAATGGAATCTGGCTATGAAAGCAGTGAGAGGAACAGCAGCAGCCCTGTCAGCCTGGATGCAGCCCTGCCTGAGAGCTCAAATGTCTACAGGGATCCAAGTGCTAAGAGATCAGCTGGGTTGGTTCCTTCCTGGCGTCATATCCCAAAGTCGCACAGCAGTAGCATCCTGGAGGTAGACTCCACAGCATCCATGGGTGGCTGGACAAAGAGTCAGCCTTTCTCTGGTGAGGAGATATCTTCTAAAAGTGAACTGGATGAATTGCAGGAAGAGGTGGCCAGGAGGGCGCAGGAACAGGAACTTCGAAGAAAACGGGAGAAGGAGTTAGAGGCAGCGAAAGGGTTTAACCCTCATCCTAGCCGCTTCATGGACTTGGATGAACTGCAGAATCAGGGGAGGAGTGACGGCTTTGAGAGGTCCCTGCAAGAGGCAGAGTCAGTGTTTGAAGAGTCACTACATCTGGAACAGAAAGGAGACTGTGCTGCAGCTTTGGCTCTCTGTAATGAAGCTATCTCTAAACTAAGACTTGCCCTGCATGGTGCCAGCTGTAGCACGCACAGCAGAGCCCTAGTCGATAAGAAGTTGCAAATCAGTATTCGAAAAGCACGGAGCCTGCAGGATCGCATGCAGCAGCAGCAATCACCACAGCAGCCGTCGCAGCCCTCAGCCTGCCTCCCAACACAGGCGGGGACTCTCTCTCAGCCAACAAGTTCCTTTGGCCGGGCTGCCTCTCAGGACCCCAGTGAACAGCCTATCCCGCTCCAAGTATTGTTAAGCCAAGAGGCCCAACTGGAATCCGGCATGGATACAGAGTTTGGGGCCAGTTCTTTCTTCCATTCACCTGCTTCCTGCCATGAGTCACACTCATCACTATCTCCAGAGTCATCTGCCCCACAGCACAGCTCCCCCAGTAGGTCTGCCTTGAAGCTTCTGACTTCGGTTGAAGTAGACAACATTGAACCCTCTGCATTCCACAGGCAAGGTTTACCTAAAGCACCAGGGTGGACTGAGAAGAATTCTCATCATAGTTGGGAGCCATTGGATGCCCCAGAGGGTAAGCTGCAAGGCTCTAGGTGTGACAACAGCAGTTGCAGCAAGCTCCCTCCACAAGAAGGAAGAGGCATTGCTCAAGAACAGCTGTTCCAAGAAAAGAAGGATCCTGCTAACCCCTCCCCGGTGATGCCTGGAATAGCCACCTCTGAGAGGGGTGATGAACACAGCCTAGGCTGTAGTCCTTCAAATTCATCAGCTCAGCCCAGCCTTCCCCTGTATAGAACCTGCCACCCCATAATGCCTGTTGCTTCTTCATTTGTGCTTCACTGTCCTGATCCTGTGCAGAAAACTAACCAATGCCTCCAAGGCCAAAGCCTCAAAACTTCATTGACTTTAAAAGTGGACAGAGGCAGTGAGGAGACCTATAGGCCAGAGTTTCCCAGCACAAAGGGGCTTGTCCGTTCTCTGGCTGAGCAGTTCCAGAGGATGCAGGGTGTCTCCATGAGGGATAGTACAGGTTTCAAGGATAGAAGTTTGTCAGGTAGTCTAAGGAAGAACTCTTCCCCTTCTGATTCTAAGCCTCCTTTCTCACAGGGTCAAGAGAAAGGCCACTGGCCATGGGCAAAGCAACAATCCTCTCTGGAGGGTGGGGATAGACCACTTTCCTGGGAAGAGTCCACTGAACATTCTTCTCTTGCCTTAAACTCTGGGCTGCCTAATGGTGAAACTTCTAGCGGAGGACAGCCCAGGTTGGCAGAGCCAGACATATACCAAGAGAAGCTGTCCCAAGTGAGAGATGTTAGGTCTAAGGATCTGGGCAGCAGTACTGACTTGGGGACTTCCTTGCCTTTGGATTCCTGGGTGAATATCACAAGGTTCTGTGATTCTCAGCTTAAGCATGGGGCACCTAGGCCAGGAATGAAGTCCTCCCCTCATGATTCCCATACGTGTGTAACCTATCCAGAGAGAAATCACATCCTTTTGCATCCACATTGGAACCAAGACACAGAGCAGGAGACCTCAGAATTGGAGTCTCTGTATCAGGCCAGTCTTCAGGCTTCTCAAGCTGGCTGTTCTGGATGGGGGCAGCAGGATACCGCCTGGCACCCACTTAGCCAAACAGGCTCTGCAGATGGCATGGGGAGGAGGTTGCACTCAGCCCATGATCCTGGTCTCTCAAAGACTTCAACAGCAGAAATGGAGCATGGTCTCCATGAAGCCAGAACAGTGCGTACTTCTCAGGCTACACCTTGCCGAGGCCTCAGCAGGGAGTGTGGGGAGGATGAGCAGTACAGTGCAGAGAATTTACGTCGCATCTCACGCAGTCTCAGTGGCACCGTTGTCTCAGAGAGGGAGGAAGCTCCGGTTTCTTCCCACAGTTTTGATTCATCAAACGTGAGGAAGCCTTTGGAAACCGGGCACCGTTGTTCCAGCTCCTCTTCCCTCCCTGTCATCCATGACCCTTCTGTGTTTCTCCTCGGTCCCCAACTCTACCTTCCCCAACCACAGTTCCTGTCCCCAGATGTCCTGATGCCCACCATGGCAGGGGAGCCCAATAGACTCCCAGGAACTTCAAGGAGTGTCCAGCAGTTTCTGGCTATGTGTGACAGGGGTGAAACTTCCCAAGGGGCCAAGTACACAGGAAGGACTTTGAACTACCAGAGCCTCCCCCATCGCTCCAGAACAGACAACTCCTGGGCACCCTGGTCAGAGACCAACCAGCATATTGGGACCAGATTCCTGACTACTCCAGGGTGCAATCCTCAACTAACCTACACTGCCACACTACCAGAAAGAAGCAAGGGCCTTCAGGTTCCTCACACTCAGTCCTGGAGTGATCTTTTCCATTCACCCTCCCACCCTCCCATTGTTCATCCTGTGTACCCACCATCTAGCAGTCTTCATGTACCCCTGAGGTCAGCTTGGAATTCAGATCCTGTTCCAGGGTCCCGAACCCCTGGTCCTCGAAGAGTAGATATGCCCCCAGATGATGACTGGAGGCAAAGCAGTTATGCCTCCCACTCTGGACACAGGAGAACAGTGGGAGAGGGGTTTCTGTTTGTTCTATCAGATGCTCCCAGAAGAGAGCAGATCAGGGCTAGAGTCCTGCAGCACAGTCAATGGGCGGCCGC

Amino Acid Sequence

MSWKRNYFSGGRGSVQGMFAPRSSTSIAPSKGLSNEPGQNSCFLNSALQV
LWHLDIFRRSFRQLTTHKCMGDSCIFCALKGIFNQFQCSSEKVLPSDTLR
SALAKTFQDEQRFQLGIMDDAAECFENLLMRIHFHIADETKEDICTAQHC
ISHQKFAMTLFEQCVCTSCGATSDPLPFIQMVHYISTTSLCNQAICMLER
REKPSPSMFGELLQNASTMGDLRNCPSNCGERIRIRRVLMNAPQIITIGL
VWDSDHSDLAEDVIHSLGTCLKLGDLFFRVTDDRAKQSELYLVGMICYYG
KHYSTFFFQTKIRKWMYFDDAHVKEIGPKWKDVVTKCIKGHYQPLLLLYA
DPQGTPVSTQDLPPQAEFQSYSRTCYDSEDSGREPSISSDTRTDSSTESY
PYKHSHHESVVSHFSSDSQGTVIYNVENDSMSQSSRDTGHLTDSECNQKH
TSKKGSLIERKRSSGRVRRKGDEPQASGYHSEGETLKEKQAPRNASKPSS
STNRLRDFKETVSNMIHNRPSLASQTNVGSHCRGRGGDQPDKKPPRTLPL
HSRDWEIESTSSESKSSSSSKYRPTWRPKRESLNIDSIFSKDKRKHCGYT
QLSPFSEDSAKEFIPDEPSKPPSYDIKFGGPSPQYKRWGPARPGSHLLEQ
HPRLIQRMESGYESSERNSSSPVSLDAALPESSNVYRDPSAKRSAGLVPS
WRHIPKSHSSSILEVDSTASMGGWTKSQPFSGEEISSKSELDELQEEVAR
RAQEQELRRKREKELEAAKGFNPHPSRFMDLDELQNQGRSDGFERSLQEA
ESVFEESLHLEQKGDCAAALALCNEAISKLRLALHGASCSTHSRALVDKK
LQISIRKARSLQDRMQQQQSPQQPSQPSACLPTQAGTLSQPTSSFGRAAS
QDPSEQPIPLQVLLSQEAQLESGMDTEFGASSFFHSPASCHESHSSLSPE
SSAPQHSSPSRSALKLLTSVEVDNIEPSAFHRQGLPKAPGWTEKNSHHSW
EPLDAPEGKLQGSRCDNSSCSKLPPQEGRGIAQEQLFQEKKDPANPSPVM
PGIATSERGDEHSLGCSPSNSSAQPSLPLYRTCHPIMPVASSFVLHCPDP
VQKTNQCLQGQSLKTSLTLKVDRGSEETYRPEFPSTKGLVRSLAEQFQRM
QGVSMRDSTGFKDRSLSGSLRKNSSPSDSKPPFSQGQEKGHWPWAKQQSS
LEGGDRPLSWEESTEHSSLALNSGLPNGETSSGGQPRLAEPDIYQEKLSQ
VRDVRSKDLGSSTDLGTSLPLDSWVNITRFCDSQLKHGAPRPGMKSSPHD
SHTCVTYPERNHILLHPHWNQDTEQETSELESLYQASLQASQAGCSGWGQ
QDTAWHPLSQTGSADGMGRRLHSAHDPGLSKTSTAEMEHGLHEARTVRTS
QATPCRGLSRECGEDEQYSAENLRRISRSLSGTVVSEREEAPVSSHSFDS
SNVRKPLETGHRCSSSSSLPVIHDPSVFLLGPQLYLPQPQFLSPDVLMPT
MAGEPNRLPGTSRSVQQFLAMCDRGETSQGAKYTGRTLNYQSLPHRSRTD
NSWAPWSETNQHIGTRFLTTPGCNPQLTYTATLPERSKGLQVPHTQSWSD
LFHSPSHPPIVHPVYPPSSSLHVPLRSAWNSDPVPGSRTPGPRRVDMPPD
DDWRQSSYASHSGHRRTVGEGFLFVLSDAPRREQIRARVLQHSQWAAAMV
SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTG
KLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVY
IMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYL
STQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU49060
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee