USP36

• Synonyms: DUB1
• Plasmid: pcDNA5D FRT/TO GFP USP36
• Parent Plasmid: pcDNA5D FRT/TO GFP
• Expressed: GFP-USP36
• Genbank: NM_025090.3 GI:122114650 with silent change c1863t
• Tag: GFP
• Species: Human

Sequence of Insert

GGATCCATGCCAATAGTGGATAAGTTGAAGGAGGCCCTGAAACCCGGCCGCAAGGACTCGGCTGATGATGGAGAACTGGGGAAGCTTCTTGCCTCCTCTGCCAAGAAGGTCCTTTTACAGAAAATCGAGTTCGAGCCAGCCAGCAAGAGCTTCTCCTACCAGCTGGAGGCCTTAAAGAGCAAATATGTGTTGCTCAACCCCAAAACAGAGGGAGCTAGTCGCCACAAGAGTGGAGATGACCCACCGGCCAGGAGACAGGGCAGTGAGCACACGTATGAGAGCTGTGGTGACGGAGTCCCAGCCCCGCAGAAAGTGCTTTTCCCCACGGAGCGACTGTCTCTGAGGTGGGAGCGGGTCTTCCGCGTGGGCGCAGGACTCCACAACCTTGGCAACACCTGCTTTCTCAATGCCACCATCCAGTGCTTGACCTACACACCACCTCTAGCCAACTACCTGCTCTCCAAGGAGCATGCTCGCAGCTGCCACCAGGGAAGCTTCTGCATGCTGTGTGTCATGCAGAACCACATTGTCCAGGCCTTCGCCAACAGCGGCAACGCCATCAAGCCCGTCTCCTTCATCCGAGACCTGAAAAAGATCGCCCGACACTTCCGCTTTGGGAACCAGGAGGACGCGCATGAGTTCCTGCGGTACACCATCGACGCCATGCAGAAAGCCTGCCTGAATGGCTGTGCCAAGTTGGATCGTCAAACGCAGGCTACTACCTTGGTCCATCAAATTTTTGGAGGGTATCTCAGATCACGCGTGAAGTGCTCCGTGTGCAAGAGCGTCTCGGACACCTACGACCCCTACTTGGACGTCGCGCTGGAGATCCGGCAAGCTGCGAATATTGTGCGTGCTCTGGAACTTTTTGTGAAAGCAGATGTCCTGAGTGGAGAGAATGCCTACATGTGTGCTAAATGCAAGAAGAAGGTTCCAGCCAGCAAGCGCTTCACCATCCACAGAACATCCAACGTCTTAACCCTTTCCCTCAAGCGCTTTGCCAACTTCAGCGGGGGGAAGATCACCAAGGATGTAGGCTATCCGGAATTCCTCAACATACGTCCGTATATGTCCCAGAATAATGGTGATCCTGTCATGTATGGACTCTATGCTGTCCTGGTGCACTCGGGCTACAGCTGCCATGCCGGGCACTATTACTGCTACGTGAAGGCAAGCAATGGACAGTGGTACCAGATGAATGATTCCTTGGTCCATTCCAGCAACGTCAAGGTGGTTCTGAACCAGCAGGCCTACGTGCTGTTCTATCTGCGAATTCCAGGCTCTAAGAAAAGTCCCGAGGGCCTCATCTCCAGGACAGGCTCCTCCTCCCTTCCCGGCCGCCCGAGTGTGATTCCAGATCACTCCAAGAAGAACATCGGCAATGGGATTATTTCCTCCCCACTGACTGGAAAGCGACAAGACTCTGGGACGATGAAGAAGCCGCACACCACTGAAGAGATTGGTGTGCCCATATCCAGGAATGGCTCCACCCTGGGCCTGAAGTCCCAGAACGGCTGCATTCCTCCAAAGCTGCCCTCGGGGTCCCCTTCCCCCAAACTCTCCCAGACACCCACACACATGCCAACCATCCTAGACGACCCTGGAAAGAAGGTGAAGAAGCCAGCTCCTCCACAGCACTTTTCCCCCAGAACTGCTCAGGGGCTGCCTGGGACCAGCAACTCGAATAGCAGCAGATCTGGGAGCCAAAGGCAGGGCTCCTGGGACAGCAGGGATGTTGTCCTCTCTACCTCACCTAAGCTCCTGGCTACAGCCACTGCCAACGGGCATGGGCTGAAGGGGAACGACGAGAGCGCTGGCCTCGACAGGAGGGGCTCCAGCAGCTCCAGCCCAGAGCACTCGGCCAGCAGTGACTCCACCAAGGCCCCCCAGACCCCCAGGAGTGGAGCGGCCCATCTCTGCGATTCTCAGGAAACGAACTGTTCCACCGCTGGCCACTCCAAAACGCCGCCAAGTGGAGCAGATTCTAAGACGGTGAAGCTGAAGTCCCCTGTCCTGAGCAACACCACCACTGAGCCTGCAAGCACCATGTCTCCTCCACCAGCCAAAAAACTGGCCCTTTCTGCCAAGAAGGCCAGCACCCTGTGGAGGGCGACCGGCAATGACCTCCGTCCACCTCCCCCCTCACCATCCTCCGACCTCACCCACCCCATGAAAACCTCTCACCCCGTCGTTGCCTCCACTTGGCCCGTCCATAGAGCCAGGGCTGTGTCACCTGCTCCCCAATCATCCAGCCGCCTGCAACCCCCCTTCAGCCCCCACCCCACATTGCTGTCCAGTACCCCCAAGCCCCCAGGGACGTCAGAACCACGGAGCTGCTCCTCCATCTCGACGGCGCTGCCTCAGGTCAACGAGGACCTTGTGTCTCTTCCACACCAGTTGCCAGAGGCCAGTGAGCCCCCCCAGAGCCCCTCTGAGAAGAGGAAAAAGACCTTTGTGGGAGAGCCGCAGAGGCTGGGCTCAGAGACGCGCCTCCCACAGCACATCAGGGAGGCCACTGCGGCTCCCCACGGGAAGAGGAAGAGGAAGAAGAAGAAGCGCCCGGAGGACACAGCTGCCAGCGCCCTGCAGGAGGGGCAGACACAGAGACAGCCTGGGAGCCCCATGTACAGGAGGGAGGGCCAGGCACAGCTGCCCGCTGTCAGACGGCAGGAAGATGGCACACAGCCACAGGTGAATGGCCAGCAGGTGGGATGTGTTACGGACGGCCACCACGCGAGCAGCAGGAAGCGGAGGAGGAAAGGAGCAGAAGGTCTTGGTGAAGAAGGCGGCCTGCACCAGGACCCACTTCGGCACAGCTGCTCTCCCATGGGTGATGGTGATCCAGAGGCCATGGAAGAGTCTCCAAGGAAAAAGAAAAAGAAAAAAAGAAAGCAGGAGACACAGCGGGCAGTAGAAGAGGATGGGCATCTCAAATGCCCAAGGAGTGCCAAGCCCCAAGATGCTGTTGTCCCCGAGTCCAGCAGCTGCGCACCATCCGCGAATGGCTGGTGTCCTGGGGACCGCATGGGGCTGAGCCAGGCCCCTCCTGTGTCTTGGAATGGAGAGCGGGAGTCTGATGTGGTCCAGGAACTGCTCAAATACTCATCTGATAAAGCTTACGGGAGAAAAGTTCTGACCTGGGATGGCAAGATGTCGGCGGTCAGTCAGGATGCTATTGAAGACAGCAGACAGGCCCGGACTGAGACCGTGGTTGATGACTGGGACGAAGAGTTTGACCGAGGGAAGGAAAAGAAAATTAAAAAATTTAAGAGAGAGAAGAGGAGAAACTTCAACGCCTTCCAGAAACTTCAGACTCGACGGAACTTCTGGTCTGTGACTCACCCAGCAAAGGCTGCCAGCCTCAGCTATCGCCGCTAAGGGCGAATTCCACATTGGTCGCTGCAGCCCGGGGGATCC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSMPIVDK
LKEALKPGRKDSADDGELGKLLASSAKKVLLQKIEFEPASKSFSYQLEAL
KSKYVLLNPKTEGASRHKSGDDPPARRQGSEHTYESCGDGVPAPQKVLFP
TERLSLRWERVFRVGAGLHNLGNTCFLNATIQCLTYTPPLANYLLSKEHA
RSCHQGSFCMLCVMQNHIVQAFANSGNAIKPVSFIRDLKKIARHFRFGNQ
EDAHEFLRYTIDAMQKACLNGCAKLDRQTQATTLVHQIFGGYLRSRVKCS
VCKSVSDTYDPYLDVALEIRQAANIVRALELFVKADVLSGENAYMCAKCK
KKVPASKRFTIHRTSNVLTLSLKRFANFSGGKITKDVGYPEFLNIRPYMS
QNNGDPVMYGLYAVLVHSGYSCHAGHYYCYVKASNGQWYQMNDSLVHSSN
VKVVLNQQAYVLFYLRIPGSKKSPEGLISRTGSSSLPGRPSVIPDHSKKN
IGNGIISSPLTGKRQDSGTMKKPHTTEEIGVPISRNGSTLGLKSQNGCIP
PKLPSGSPSPKLSQTPTHMPTILDDPGKKVKKPAPPQHFSPRTAQGLPGT
SNSNSSRSGSQRQGSWDSRDVVLSTSPKLLATATANGHGLKGNDESAGLD
RRGSSSSSPEHSASSDSTKAPQTPRSGAAHLCDSQETNCSTAGHSKTPPS
GADSKTVKLKSPVLSNTTTEPASTMSPPPAKKLALSAKKASTLWRATGND
LRPPPPSPSSDLTHPMKTSHPVVASTWPVHRARAVSPAPQSSSRLQPPFS
PHPTLLSSTPKPPGTSEPRSCSSISTALPQVNEDLVSLPHQLPEASEPPQ
SPSEKRKKTFVGEPQRLGSETRLPQHIREATAAPHGKRKRKKKKRPEDTA
ASALQEGQTQRQPGSPMYRREGQAQLPAVRRQEDGTQPQVNGQQVGCVTD
GHHASSRKRRRKGAEGLGEEGGLHQDPLRHSCSPMGDGDPEAMEESPRKK
KKKKRKQETQRAVEEDGHLKCPRSAKPQDAVVPESSSCAPSANGWCPGDR
MGLSQAPPVSWNGERESDVVQELLKYSSDKAYGRKVLTWDGKMSAVSQDA
IEDSRQARTETVVDDWDEEFDRGKEKKIKKFKREKRRNFNAFQKLQTRRN
FWSVTHPAKAASLSYRR*

• Vector Type: Mammalian
• Antibiotic: Amp
• Full Snapgene Map: pcDNA5D FRT/TO GFP USP36 141.99 KBSnapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU49030
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee