Nemo (mouse)

• Plasmid: pRetrox tight HA Nemo (mouse) K269A
• Parent Plasmid: pRetroX tight puro
• Expressed: HA-NEMO (mouse) K270A
• Genbank: NEMO
• Tag: HA
• Species: Mouse

Sequence of Insert

GGATCCATGAACAAGCACCCCTGGAAGAACCAGCTGAGTGAGATGGTGCA
GCCCAGTGGTGGCCCAGCAGAGGACCAGGACATGCTGGGTGAAGAATCTT
CTCTGGGGAAGCCTGCAATGCTACATCTGCCTTCAGAGCAGGGTACTCCT
GAGACCCTCCAGCGCTGCCTGGAAGAGAATCAAGAGCTCCGAGACGCTAT
CCGGCAGAGCAATCAGATGCTGAGGGAACGCTGTGAGGAGCTGCTGCATT
TCCAGGTCAGCCAGCGGGAGGAGAAGGAGTTCCTTATGTGCAAATTCCAG
GAAGCCCGGAAGCTGGTGGAGAGACTGAGCTTGGAGAAGCTTGATCTTCG
GAGTCAGAGGGAACAGGCCTTAAAGGAGTTGGAGCAACTGAAGAAATGCC
AACAGATGGCTGAGGACAAGGCCTCTGTGAAAGCTCAGGTGACATCATTG
CTCGGAGAACTCCAGGAGAGCCAGAGCCGTTTGGAGGCTGCCACCAAGGA
TCGGCAAGCTTTAGAGGGAAGGATTCGAGCAGTTAGTGAGCAGGTCAGAC
AGCTGGAGAGTGAGCGGGAGGTGCTACAGCAGCAGCACAGCGTCCAGGTG
GACCAGCTGCGTATGCAGAACCAGAGCGTGGAGGCTGCCTTGCGAATGGA
GCGGCAGGCTGCTTCAGAGGAGAAGCGGAAGCTGGCTCAGTTGCAGGCAG
CCTATCACCAGCTCTTCCAAGACTACGACAGCCACATTAAGAGCAGCAAG
GGCATGCAGCTGGAAGATCTGAGGCAACAGCTCCAGCAAGCTGAGGAGGC
CCTGGTAGCCGCACAGGAATTGATTGATAAGCTGAAAGAGGAGGCTGAGC
AGCACAAGATTGTGATGGAGACTGTGCCAGTCTTGAAGGCCCAGGCGGAT
ATCTACAAGGCTGACTTCCAAGCTGAGAGGCATGCCCGGGAGAAGCTGGT
GGAGAAGAAGGAGTATTTGCAGGAGCAGCTGGAGCAGCTGCAGCGCGAGT
TCAACAAGCTGAAAGTTGGCTGCCATGAGTCAGCCAGGATTGAGGATATG
AGGAAGCGGCATGTAGAGACTCCCCAGCCTCCTTTACTCCCTGCTCCAGC
TCACCACTCCTTTCATTTGGCCTTGTCCAACCAGCGGAGGAGCCCTCCTG
AAGAACCTCCTGACTTCTGTTGTCCGAAGTGCCAGTATCAGGCTCCTGAT
ATGGACACTCTACAGATACATGTCATGGAGTGCATAGAGTAGGCGGCCGC

Amino Acid Sequence

MYPYDVPDYAGSMNKHPWKNQLSEMVQPSGGPAEDQDMLGEESSLGKPAM LHLPSEQGTPETLQRCLEENQELRDAIRQSNQMLRERCEELLHFQVSQRE EKEFLMCKFQEARKLVERLSLEKLDLRSQREQALKELEQLKKCQQMAEDK ASVKAQVTSLLGELQESQSRLEAATKDRQALEGRIRAVSEQVRQLESERE VLQQQHSVQVDQLRMQNQSVEAALRMERQAASEEKRKLAQLQAAYHQLFQ DYDSHIKSSKGMQLEDLRQQLQQAEEALVAAQELIDKLKEEAEQHKIVME TVPVLKAQADIYKADFQAERHAREKLVEKKEYLQEQLEQLQREFNKLKVG CHESARIEDMRKRHVETPQPPLLPAPAHHSFHLALSNQRRSPPEEPPDFC CPKCQYQAPDMDTLQIHVMECIE*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU46225
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee