VCPIP1

• Synonyms: DUBA3; VCIP135
• Plasmid: pGEX TEV VCPIP1
• Parent Plasmid: pGEX TEV
• Expressed: GST-TEV-VCPIP1
• Genbank: NM_025054.4
• Tag: GST
• Species: Human

Sequence of Insert

agatctATGTCTCAGCCGCCGCCGCCGCCGCCTCCGTTGCCGCCGCCACCTCCTCCCCCTGAGGCTCCACAGACTCCGTCGTCCTTGGCGTCGGCGGCTGCTTCGGGGGGGCTTTTGAAGCGGAGAGACCGGAGAATCCTTTCCGGGAGCTGCCCGGATCCGAAGTGTCAGGCGCGTCTATTTTTCCCGGCCTCCGGTTCTGTCAGCATCGAGTGTACCGAGTGCGGCCAGCGGCACGAGCAGCAACAGCTGCTGGGGGTTGAGGAGGTGACCGACCCGGACGTAGTGCTACACAACCTGCTGCGGAACGCGCTGCTCGGGGTTACGGGGGCACCCAAGAAGAACACGGAACTGGTAAAGGTGATGGGCCTTTCCAACTATCACTGCAAATTGTTGTCGCCCATATTAGCTCGCTATGGAATGGACAAACAGACAGGCCGGGCCAAGCTTCTCCGGGACATGAACCAGGGCGAACTGTTCGATTGCGCCTTACTGGGTGACCGCGCCTTCCTCATAGAACCAGAGCATGTTAACACTGTGGGCTATGGCAAGGACCGCTCCGGAAGCCTCCTGTATTTGCATGACACTCTGGAGGACATTAAGCGGGCCAATAAAAGCCAGGAATGTCTCATTCCAGTGCATGTGGACGGGGATGGACACTGCTTGGTGCATGCTGTGTCTCGGGCTCTAGTAGGCCGAGAGCTCTTCTGGCATGCCTTAAGAGAGAATCTTAAACAGCACTTTCAGCAGCACCTGGCCCGATATCAAGCTCTGTTCCATGACTTCATTGATGCTGCTGAGTGGGAGGACATTATCAATGAGTGTGACCCTCTGTTTGTACCACCTGAGGGTGTTCCCTTGGGCCTGAGGAATATCCACATATTTGGTCTTGCCAATGTGCTACATCGTCCTATTATTCTGTTAGATTCCCTCAGTGGCATGAGAAGCTCTGGTGATTATTCAGCCACCTTTCTACCTGGGCTCATCCCTGCAGAGAAGTGCACTGGGAAAGATGGTCATTTGAACAAACCAATCTGTATTGCATGGAGCAGCTCCGGTAGAAACCATTATATCCCCTTGGTAGGCATAAAAGGGGCTGCTTTGCCCAAACTGCCTATGAATTTGCTTCCTAAAGCATGGGGTGTGCCTCAGGACCTTATTAAAAAGTACATAAAACTTGAAGAGGATGGTGGTTGTGTTATTGGAGGTGACAGAAGTTTGCAAGATAAATACTTACTTAGGCTTGTTGCTGCTATGGAAGAAGTCTTTATGGACAAACATGGTATCCATCCTAGTTTGGTTGCTGATGTCCATCAGTATTTCTACAGAAGGACTGGAGTGATAGGAGTTCAGCCTGAGGAAGTCACAGCAGCTGCTAAAAAAGCAGTAATGGATAATCGCCTTCACAAATGTTTGCTCTGTGGTGCCCTTTCTGAACTTCATGTTCCTCCAGAGTGGTTGGCTCCTGGAGGGAAATTGTATAACCTGGCAAAAAGTACTCATGGACAGCTGAGGACTGACAAAAATTACAGCTTTCCCTTGAACAATTTGGTTTGCTCATATGATTCAGTGAAAGATGTTCTGGTACCAGACTATGGAATGAGTAACCTAACAGCTTGTAATTGGTGCCATGGCACATCTGTGCGAAAGGTCAGAGGAGATGGGTCTATTGTGTATTTGGATGGAGACAGAACTAATTCTAGGTCCACTGGTGGCAAATGTGGTTGTGGATTCAAACACTTTTGGGATGGTAAGGAGTATGACAATCTACCAGAAGCTTTCCCTATTACTTTAGAATGGGGTGGAAGAGTGGTCAGAGAAACAGTATATTGGTTCCAGTATGAAAGTGATTCATCTTTGAATAGTAATGTTTACGATGTTGCAATGAAACTTGTTACCAAGCACTTTCCAGGTGAATTTGGGAGTGAAATCCTAGTTCAGAAAGTTGTCCACACTATATTGCATCAGACTGCCAAAAAGAATCCCGATGATTATACTCCTGTAAATATAGATGGTGCTCACGCCCAAAGAGTTGGAGATGTTCAAGGACAAGAATCAGAGTCTCAGCTCCCAACTAAAATTATTCTTACTGGACAGAAAACAAAAACTTTGCACAAGGAGGAGTTAAACATGAGTAAAACTGAAAGAACTATTCAACAGAATATTACGGAACAGGCTTCTGTAATGCAGAAACGGAAAACAGAGAAGTTAAAACAAGAACAAAAAGGGCAACCCAGGACTGTTTCTCCCAGTACCATTCGTGATGGTCCATCCTCTGCACCTGCTACACCTACCAAGGCTCCCTATTCACCGACAACTTCTAAGGAGAAGAAGATCCGAATCACAACTAATGATGGACGACAGTCCATGGTTACCCTTAAGTCTTCAACAACCTTTTTTGAACTTCAGGAAAGTATAGCCAGAGAATTCAACATTCCTCCATATTTACAGTGTATTCGATACGGGTTTCCTCCTAAAGAGTTAATGCCACCACAGGCAGGAATGGAAAAGGAACCAGTTCCTTTACAGCATGGCGACAGAATTACAATAGAAATTCTAAAAAGTAAAGCTGAAGGTGGTCAGTCTGCTGCAGCACACTCAGCCCACACTGTGAAACAAGAAGATATTGCTGTTACTGGTAAACTGTCATCTAAGGAACTTCAGGAGCAAGCTGAAAAAGAAATGTACTCCTTGTGTCTTTTAGCAACATTAATGGGAGAAGATGTATGGTCTTATGCAAAGGGACTTCCTCACATGTTTCAGCAGGGTGGTGTATTCTACAGTATTATGAAGAAAACCATGGGTATGGCTGATGGCAAGCATTGTACTTTTCCACATCTGCCTGGCAAAACCTTTGTCTATAATGCTTCTGAAGATAGACTGGAATTGTGTGTGGATGCTGCAGGACATTTCCCCATTGGTCCTGATGTTGAAGATTTAGTTAAAGAGGCTGTAAGTCAGGTTCGAGCAGAGGCTACTACAAGAAGTAGGGAATCAAGTCCCTCACATGGGCTATTAAAACTAGGTAGTGGTGGAGTAGTGAAAAAGAAATCTGAGCAACTTCATAACGTAACTGCCTTTCAGGGAAAAGGGCATTCTTTAGGAACTGCATCTGGTAACCCACACCTTGATCCAAGAGCTAGGGAAACTTCAGTTGTAAGAAAGCATAATACAGGGACAGACTTTAGTAATAGTTCCACTAAAACAGAGCCTTCTGTATTCACAGCTTCTTCTAGTAATAGTGAGCTTATTCGAATAGCTCCTGGAGTAGTAACAATGAGAGACGGCAGGCAGCTTGATCCTGATTTGGTTGAGGCCCAGCGAAAAAAATTGCAGGAAATGGTTTCTTCTATTCAGGCTTCAATGGACAGGCACCTTCGGGATCAAAGTACAGAGCAGTCACCATCTGATCTTCCTCAAAGGAAAACAGAAGTTGTGAGTTCTTCTGCAAAGTCTGGGAGTCTTCAGACTGGTTTGCCTGAATCTTTTCCTTTAACTGGTGGTACTGAAAATTTGAATACAGAAACAACTGATGGCTGTGTAGCAGATGCACTGGGAGCAGCCTTTGCCACAAGGTCAAAAGCACAAAGGGGAAATTCCGTGGAGGAGCTTGAAGAGATGGATAGTCAAGATGCTGAGATGACTAACACAACTGAGCCAATGGATCACTCTTGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDENLYFQGGSMSQPPPPPPPLPPPPPPPEAP
QTPSSLASAAASGGLLKRRDRRILSGSCPDPKCQARLFFPASGSVSIECT
ECGQRHEQQQLLGVEEVTDPDVVLHNLLRNALLGVTGAPKKNTELVKVMG
LSNYHCKLLSPILARYGMDKQTGRAKLLRDMNQGELFDCALLGDRAFLIE
PEHVNTVGYGKDRSGSLLYLHDTLEDIKRANKSQECLIPVHVDGDGHCLV
HAVSRALVGRELFWHALRENLKQHFQQHLARYQALFHDFIDAAEWEDIIN
ECDPLFVPPEGVPLGLRNIHIFGLANVLHRPIILLDSLSGMRSSGDYSAT
FLPGLIPAEKCTGKDGHLNKPICIAWSSSGRNHYIPLVGIKGAALPKLPM
NLLPKAWGVPQDLIKKYIKLEEDGGCVIGGDRSLQDKYLLRLVAAMEEVF
MDKHGIHPSLVADVHQYFYRRTGVIGVQPEEVTAAAKKAVMDNRLHKCLL
CGALSELHVPPEWLAPGGKLYNLAKSTHGQLRTDKNYSFPLNNLVCSYDS
VKDVLVPDYGMSNLTACNWCHGTSVRKVRGDGSIVYLDGDRTNSRSTGGK
CGCGFKHFWDGKEYDNLPEAFPITLEWGGRVVRETVYWFQYESDSSLNSN
VYDVAMKLVTKHFPGEFGSEILVQKVVHTILHQTAKKNPDDYTPVNIDGA
HAQRVGDVQGQESESQLPTKIILTGQKTKTLHKEELNMSKTERTIQQNIT
EQASVMQKRKTEKLKQEQKGQPRTVSPSTIRDGPSSAPATPTKAPYSPTT
SKEKKIRITTNDGRQSMVTLKSSTTFFELQESIAREFNIPPYLQCIRYGF
PPKELMPPQAGMEKEPVPLQHGDRITIEILKSKAEGGQSAAAHSAHTVKQ
EDIAVTGKLSSKELQEQAEKEMYSLCLLATLMGEDVWSYAKGLPHMFQQG
GVFYSIMKKTMGMADGKHCTFPHLPGKTFVYNASEDRLELCVDAAGHFPI
GPDVEDLVKEAVSQVRAEATTRSRESSPSHGLLKLGSGGVVKKKSEQLHN
VTAFQGKGHSLGTASGNPHLDPRARETSVVRKHNTGTDFSNSSTKTEPSV
FTASSSNSELIRIAPGVVTMRDGRQLDPDLVEAQRKKLQEMVSSIQASMD
RHLRDQSTEQSPSDLPQRKTEVVSSSAKSGSLQTGLPESFPLTGGTENLN
TETTDGCVADALGAAFATRSKAQRGNSVEELEEMDSQDAEMTNTTEPMDH
S*AA

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU25041
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee