UBE2K

• Plasmid: pGEX6P UBE2K 1-159 -Linker 2-TEV-Ubiquitin 1-75-HA
• Parent Plasmid: pGEX6P1
• Expressed: GST-C3-UBE2K 1-159 -Linker 2-TEV-Ubiquitin 1-75-HA
• Genbank: NP_005330.1
• Tag: GST
• Tag: HA
• Species: Human

Sequence of Insert

GGATCCATGGCCAACATCGCGGTGCAGCGAATCAAGCGGGAGTTCAAGGAGGTGCTGAAGAGCGAGGAGACGAGCAAAAATCAAATTAAAGTAGATCTTGTAGATGAGAATTTTACAGAATTAAGAGGAGAAATAGCAGGACCTCCAGACACACCATATGAAGGAGGAAGATACCAACTAGAGATAAAAATACCAGAAACATACCCATTTAATCCCCCTAAGGTCCGGTTTATCACTAAAATATGGCATCCTAATATTAGTTCCGTCACAGGGGCTATTTGTTTGGATATCCTGAAAGATCAATGGGCAGCTGCAATGACTCTCCGCACGGTATTATTGTCATTGCAAGCACTATTGGCAGCTGCAGAGCCAGATGATCCACAGGATGCTGTAGTAGCAAATCAGTACAAACAAAATCCCGAAATGTTCAAACAGACAGCTCGACTTTGGGCACATGTGTATGCTGGAGCACCAGTTTCTAGTGGAGATGACGTTGATGAAAACCTGTATTTTCAGATGCAAATCTTCGTGAAGACCCTGACTGGTAAGACCATCACTCTCGAAGTGGAGCCGAGTGACACCATTGAGAATGTCAAGGCAAAGATCCAAGACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACGCACCCTGTCTGACTACAACATCCAGAAAGAGTCCACCCTGCACCTGGTCCTCCGTCTCAGAGGTTACCCATACGATGTGCCAGATTACGCCTAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMANIAVQRIKREFKEVLKS
EETSKNQIKVDLVDENFTELRGEIAGPPDTPYEGGRYQLEIKIPETYPFN
PPKVRFITKIWHPNISSVTGAICLDILKDQWAAAMTLRTVLLSLQALLAA
AEPDDPQDAVVANQYKQNPEMFKQTARLWAHVYAGAPVSSGDDVDENLYF
QMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQ
LEDGRTLSDYNIQKESTLHLVLRLRGYPYDVPDYA*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU45484
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee