cullin3

• Synonyms: CUL3, CUL-3, PHA2E
• Plasmid: pcDNA5-FRT/TO GFP CULLIN3 delExon9
• Parent Plasmid: pcDNA5 FRT/TO GFP
• Expressed: GFP-Cullin3 delta Exon9
• Genbank: NP_003581.1
• Tag: GFP
• Species: Human

Sequence of Insert

GGATCCATGTCGAATCTGAGCAAAGGCACGGGCAGCCGGAAGGACACCAA
GATGCGTATCCGGGCCTTTCCGATGACCATGGATGAAAAATATGTAAACA
GCATTTGGGACCTTCTGAAAAATGCAATTCAAGAAATCCAGCGTAAGAAT
AACAGTGGTCTTAGTTTTGAGGAGCTCTATAGAAATGCATATACAATGGT
TTTGCATAAACATGGAGAAAAGCTCTACACTGGACTAAGAGAAGTTGTTA
CCGAACATCTCATAAATAAGGTGCGAGAAGATGTACTAAATTCATTGAAT
AACAACTTTCTTCAAACGCTAAATCAAGCTTGGAATGATCATCAAACAGC
TATGGTGATGATTAGAGACATACTAATGTACATGGACCGTGTGTATGTAC
AACAAAATAATGTGGAGAACGTCTACAATTTGGGATTAATTATTTTTCGA
GATCAAGTTGTACGTTATGGGTGTATTAGGGATCATCTACGGCAAACTCT
ATTGGATATGATTGCAAGAGAGCGGAAAGGAGAAGTCGTAGACAGAGGCG
CAATAAGAAATGCTTGCCAGATGTTAATGATTTTAGGTCTCGAAGGAAGA
TCAGTCTATGAAGAAGATTTTGAGGCTCCTTTTTTGGAAATGTCTGCAGA
ATTTTTTCAGATGGAAAGCCAGAAATTTTTAGCAGAAAATAGTGCTTCAG
TATATATAAAGAAAGTAGAAGCTAGAATTAATGAAGAAATAGAACGAGTG
ATGCACTGCCTTGACAAATCAACGGAAGAACCAATTGTAAAGGTGGTTGA
AAGGGAACTCATTTCCAAGCACATGAAGACTATAGTAGAAATGGAGAATT
CTGGGCTAGTACATATGTTGAAAAATGGAAAGACAGAAGACCTTGGTTGC
ATGTACAAGTTATTTAGTCGTGTGCCAAATGGTTTGAAAACAATGTGTGA
GTGTATGAGTTCCTATTTGAGGGAGCAAGGTAAAGCTCTTGTTTCTGAAG
AAGGAGAAGGAAAGAATCCTGTTGACTATATCCAGGGCTTATTGGATCTG
AAGAGTAGGTTCGATCGCTTCCTCCTGGAATCATTCAACAATGACCGTCT
CTTTAAACAAACTATTGCGGGTGACTTTGAGTATTTTCTCAACCTCAACT
CCAGGTCTCCTGAATACCTCTCATTATTTATTGATGATAAGCTGAAAAAG
GGAGTCAAAGGGACTGAATGTGGATGTCAGTTCACGTCAAAACTGGAAGG
AATGTTTAGGGATATGAGCATCTCAAACACAACGATGGATGAATTCAGGC
AACATCTACAGGCAACTGGTGTATCTTTAGGTGGTGTTGATCTTACAGTC
CGGGTGCTCACGACAGGATATTGGCCCACTCAGTCAGCCACACCAAAGTG
CAACATCCCACCAGCACCAAGACATGCTTTTGAGATATTCAGAAGGTTCT
ACTTAGCCAAACACAGTGGTCGACAGCTCACACTCCAGCATCATATGGGT
TCTGCAGATCTCAATGCCACATTTTATGGACCAGTTAAAAAGGAAGATGG
ATCTGAAGTTGGTGTTGGAGGTGCACAAGTAACTGGCTCTAATACACGGA
AGCACATATTGCAAGTTTCCACTTTCCAGATGACCATATTAATGCTCTTT
AATAATAGAGAAAAATACACATTTGAGGAAATTCAGCAAGAGACAGATAT
CCCTGAAAGAGAGCTTGTTAGAGCCCTACAGTCCCTCGCCTGTGGTAAAC
CAACACAGCGGGTTCTTACAAAAGAACCCAAATCAAAGGAAATAGAAAAT
GGTCATATATTTACAGTTAATGATCAATTCACATCCAAACTACACAGAGT
CAAGATTCAAACAGTTGCTGCCAAACAAGGTGAATCCGACCCAGAGAGGA
AAGAAACAAGGCAGAAAGTAGACGACGACAGAAAACATGAGATAGAAGCT
GCTATAGTGCGGATAATGAAATCTAGAAAGAAGATGCAGCACAATGTTCT
AGTAGCGGAGGTAACTCAGCAGTTGAAGGCGCGATTCTTACCAAGTCCAG
TTGTTATTAAGAAACGTATTGAAGGACTTATTGAGAGAGAATATTTGGCA
CGAACACCTGAGGATCGCAAAGTATACACATATGTAGCATAAGCGGCCGC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSMSNLSK
GTGSRKDTKMRIRAFPMTMDEKYVNSIWDLLKNAIQEIQRKNNSGLSFEE
LYRNAYTMVLHKHGEKLYTGLREVVTEHLINKVREDVLNSLNNNFLQTLN
QAWNDHQTAMVMIRDILMYMDRVYVQQNNVENVYNLGLIIFRDQVVRYGC
IRDHLRQTLLDMIARERKGEVVDRGAIRNACQMLMILGLEGRSVYEEDFE
APFLEMSAEFFQMESQKFLAENSASVYIKKVEARINEEIERVMHCLDKST
EEPIVKVVERELISKHMKTIVEMENSGLVHMLKNGKTEDLGCMYKLFSRV
PNGLKTMCECMSSYLREQGKALVSEEGEGKNPVDYIQGLLDLKSRFDRFL
LESFNNDRLFKQTIAGDFEYFLNLNSRSPEYLSLFIDDKLKKGVKGTECG
CQFTSKLEGMFRDMSISNTTMDEFRQHLQATGVSLGGVDLTVRVLTTGYW
PTQSATPKCNIPPAPRHAFEIFRRFYLAKHSGRQLTLQHHMGSADLNATF
YGPVKKEDGSEVGVGGAQVTGSNTRKHILQVSTFQMTILMLFNNREKYTF
EEIQQETDIPERELVRALQSLACGKPTQRVLTKEPKSKEIENGHIFTVND
QFTSKLHRVKIQTVAAKQGESDPERKETRQKVDDDRKHEIEAAIVRIMKS
RKKMQHNVLVAEVTQQLKARFLPSPVVIKKRIEGLIEREYLARTPEDRKV
YTYVA*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU41462
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee