Plasmid
pETDuet-6His_TEV-Rbx1, GST_TEV-Cullin3 (codon optimised bacterial)
Parent Plasmid
pETDuet1
Expressed
GST-Cullin3
Tag
GST
Sequence of Insert
View Sequence
gtcgacatgtcccctatactaggttattggaaaattaagggccttgtgcaacccactcgacttcttttggaatatcttgaagaaaaatatgaagagcatttgtatgagcgcgatgaaggtgataaatggcgaaacaaaaagtttgaattgggtttggagtttcccaatcttccttattatattgatggtgatgttaaattaacacagtctatggccatcatacgttatatagctgacaagcacaacatgttgggtggttgtccaaaagagcgtgcagagatttcaatgcttgaaggagcggttttggatattagatacggtgtttcgagaattgcatatagtaaagactttgaaactctcaaagttgattttcttagcaagctacctgaaatgctgaaaatgttcgaagatcgtttatgtcataaaacatatttaaatggtgatcatgtaacccatcctgacttcatgttgtatgacgctcttgatgttgttttatacatggacccaatgtgcctggatgcgttcccaaaattagtttgttttaaaaaacgtattgaagctatcccacaaattgataagtacttgaaatccagcaagtatatagcatggcctttgcagggctggcaagccacgtttggtggtggcgaccatcctccaaaatcggatggatccGAAAACCTGTATTTTCAGATGAGCAATCTGAGCAAAGGCACCGGTTCCCGTAAAGACACCAAAATGCGTATCCGTGCGTTCCCGATGACGATGGACGAAAAATATGTGAACAGCATTTGGGATCTGCTGAAAAACGCGATTCAGGAAATCCAACGTAAAAACAATTCAGGTCTGTCGTTTGAAGAACTGTATCGCAATGCCTACACGATGGTGCTGCATAAACACGGCGAAAAACTGTATACCGGTCTGCGTGAAGTGGTTACGGAACATCTGATTAATAAAGTCCGCGAAGACGTGCTGAACAGCCTGAACAATAACTTTCTGCAGACCCTGAATCAAGCATGGAACGATCACCAGACGGCTATGGTGATGATTCGTGACATCCTGATGTATATGGATCGCGTTTACGTCCAGCAAAATAACGTGGAAAATGTTTATAACCTGGGTCTGATTATCTTCCGTGATCAGGTCGTGCGCTACGGCTGCATCCGTGACCATCTGCGCCAAACCCTGCTGGATATGATTGCCCGTGAACGCAAAGGCGAAGTTGTGGATCGTGGTGCGATCCGCAACGCCTGTCAGATGCTGATGATTCTGGGCCTGGAAGGTCGTTCAGTGTATGAAGAAGATTTCGAAGCGCCGTTTCTGGAAATGAGTGCCGAATTTTTCCAGATGGAATCCCAAAAATTTCTGGCAGAAAATAGTGCTTCCGTTTACATCAAAAAAGTCGAAGCACGTATTAACGAAGAAATCGAACGCGTCATGCACTGCCTGGATAAAAGCACCGAAGAACCGATTGTTAAAGTGGTTGAACGCGAACTGATCAGTAAACACATGAAAACCATCGTTGAAATGGAAAATTCCGGCCTGGTCCACATGCTGAAAAACGGCAAAACCGAAGACCTGGGTTGTATGTATAAACTGTTTAGTCGTGTGCCGAATGGTCTGAAAACCATGTGCGAATGTATGAGCAGCTACCTGCGCGAACAGGGCAAAGCACTGGTCTCCGAAGAAGGCGAAGGTAAAAACCCGGTGGATTATATCCAGGGTCTGCTGGACCTGAAATCACGTTTTGATCGCTTCCTGCTGGAATCGTTCAATAACGACCGTCTGTTTAAACAGACCATTGCGGGCGATTTTGAATATTTCCTGAATCTGAACTCACGCTCGCCGGAATACCTGAGTCTGTTTATCGATGACAAACTGAAAAAAGGCGTGAAAGGTCTGACCGAACAGGAAGTTGAAACGATTCTGGACAAAGCAATGGTTCTGTTTCGTTTCATGCAGGAAAAAGATGTCTTCGAACGCTATTACAAACAACATCTGGCTCGTCGCCTGCTGACCAATAAAAGCGTGTCTGATGACAGCGAGAAAAACATGATCTCTAAACTGAAAACCGAATGCGGCTGTCAGTTCACGAGCAAACTGGAAGGCATGTTTCGTGACATGAGTATTTCCAATACCACGATGGATGAATTTCGCCAGCACCTGCAAGCGACCGGTGTGTCACTGGGCGGTGTTGATCTGACGGTGCGTGTTCTGACCACGGGTTATTGGCCGACCCAGTCGGCAACGCCGAAATGCAACATCCCGCCGGCACCGCGTCATGCATTTGAAATTTTCCGTCGCTTTTATCTGGCCAAACACTCAGGCCGCCAGCTGACCCTGCAACATCACATGGGTTCGGCAGACCTGAATGCTACGTTCTACGGCCCGGTGAAAAAAGAAGATGGCAGCGAAGTCGGTGTGGGCGGTGCCCAGGTGACCGGTAGCAACACGCGTAAACATATCCTGCAGGTTTCTACCTTCCAAATGACGATTCTGATGCTGTTTAATAACCGCGAAAAATACACCTTCGAAGAAATCCAGCAAGAAACGGATATTCCGGAACGTGAACTGGTGCGCGCGCTGCAGAGCCTGGCATGTGGTAAACCGACCCAACGCGTTCTGACGAAAGAACCGAAATCTAAAGAAATCGAAAACGGCCATATCTTCACCGTGAACGACCAGTTTACGAGTAAACTGCACCGTGTCAAAATCCAGACCGTGGCGGCCAAACAGGGTGAATCCGATCCGGAACGTAAAGAAACGCGCCAGAAAGTGGATGACGATCGCAAACATGAAATTGAAGCAGCTATTGTTCGTATCATGAAAAGCCGTAAGAAAATGCAGCACAATGTTCTGGTCGCAGAAGTTACCCAGCAACTGAAAGCTCGTTTTCTGCCGTCTCCGGTCGTGATTAAAAAACGCATCGAAGGCCTGATTGAACGTGAATACCTGGCTCGCACCCCGGAAGATCGTAAAGTCTACACCTATGTCGCCTGAgcggccgc
Amino Acid Sequence
View Sequence
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDGSENLYFQMSNLSKGTGSRKDTKMRIRAFPMTMDEKYVNSIWDLLKNAIQEIQRKNNSGLSFEELYRNAYTMVLHKHGEKLYTGLREVVTEHLINKVREDVLNSLNNNFLQTLNQAWNDHQTAMVMIRDILMYMDRVYVQQNNVENVYNLGLIIFRDQVVRYGCIRDHLRQTLLDMIARERKGEVVDRGAIRNACQMLMILGLEGRSVYEEDFEAPFLEMSAEFFQMESQKFLAENSASVYIKKVEARINEEIERVMHCLDKSTEEPIVKVVERELISKHMKTIVEMENSGLVHMLKNGKTEDLGCMYKLFSRVPNGLKTMCECMSSYLREQGKALVSEEGEGKNPVDYIQGLLDLKSRFDRFLLESFNNDRLFKQTIAGDFEYFLNLNSRSPEYLSLFIDDKLKKGVKGLTEQEVETILDKAMVLFRFMQEKDVFERYYKQHLARRLLTNKSVSDDSEKNMISKLKTECGCQFTSKLEGMFRDMSISNTTMDEFRQHLQATGVSLGGVDLTVRVLTTGYWPTQSATPKCNIPPAPRHAFEIFRRFYLAKHSGRQLTLQHHMGSADLNATFYGPVKKEDGSEVGVGGAQVTGSNTRKHILQVSTFQMTILMLFNNREKYTFEEIQQETDIPERELVRALQSLACGKPTQRVLTKEPKSKEIENGHIFTVNDQFTSKLHRVKIQTVAAKQGESDPERKETRQKVDDDRKHEIEAAIVRIMKSRKKMQHNVLVAEVTQQLKARFLPSPVVIKKRIEGLIEREYLARTPEDRKVYTYVA*
Vector Type
Bacterial
Antibiotic
Amp
DU Number
DU23253
Unit Source
Location
PPU
MTA
Price
£125.00
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