SAE1

• Synonyms: SUMO-1 Activating Enzyme E1 N Subunit
• Plasmid: pGEX-4T-2 SAE1
• Parent Plasmid: pGEX4T2
• Expressed: GST SAE1
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGGTGGAGAAGGAGGAGGCTGGCGGCGGCATTAGCGAGGAGGA
GGCGGCACAGTATGACCGGCAGATCCGCCTGTGGGGACTGGAGGCCCAGA
AACGGCTGCGGGCCTCTCGGGTGCTTCTTGTCGGCTTGAAAGGACTTGGG
GCTGAAATTGCCAAGAATCTCATCTTGGCAGGAGTGAAAGGACTGACCAT
GCTGGATCACGAACAGGTAACTCCAGAAGATCCCGGAGCTCAGTTCTTGA
TTCGTACTGGGTCTGTTGGCCGAAATAGGGCTGAAGCCTCTTTGGAGCGA
GCTCAGAATCTCAACCCCATGGTGGATGTGAAGGTGGACACTGAGGATAT
AGAGAAGAAACCAGAGTCATTTTTCACTCAATTCGATGCTGTGTGTCTGA
CTTGCTGCTCCAGGGATGTCATAGTTAAAGTTGACCAGATCTGTCACAAA
AATAGCATCAAGTTCTTTACAGGAGATGTTTTTGGCTACCATGGATACAC
ATTTGCCAATCTAGGAGAGCATGAGTTTGTAGAGGAGAAAACTAAAGTTG
CCAAAGTTAGCCAAGGAGTAGAAGATGGGCCCGACACCAAGAGAGCAAAA
CTTGATTCTTCTGAGACAACGATGGTCAAAAAGAAGGTGGTCTTCTGCCC
TGTTAAAGAAGCCCTGGAGGTGGACTGGAGCAGTGAGAAAGCAAAGGCTG
CTCTGAAGCGCACGACCTCCGACTACTTTCTCCTTCAAGTGCTCTTAAAG
TTCCGTACAGATAAAGGAAGAGATCCCAGTTCTGATACATATGAGGAAGA
TTCTGAGTTGTTGCTCCAGATACGAAATGATGTGCTTGACTCACTGGGTA
TTAGTCCTGACCTGCTTCCTGAGGACTTTGTCAGGTACTGCTTCTCCGAG
ATGGCCCCAGTGTGTGCGGTGGTTGGAGGGATTTTGGCACAGGAAATTGT
GAAGGCCCTGTCTCAGCGGGACCCTCCTCACAACAACTTCTTCTTCTTCG
ATGGCATGAAGGGGAATGGGATTGTGGAGTGCCTTGGCCCCAAGTGAGCG
GCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSMVEKEEAGGGISEEEAAQYDRQIR
LWGLEAQKRLRASRVLLVGLKGLGAEIAKNLILAGVKGLTMLDHEQVTPE
DPGAQFLIRTGSVGRNRAEASLERAQNLNPMVDVKVDTEDIEKKPESFFT
QFDAVCLTCCSRDVIVKVDQICHKNSIKFFTGDVFGYHGYTFANLGEHEF
VEEKTKVAKVSQGVEDGPDTKRAKLDSSETTMVKKKVVFCPVKEALEVDW
SSEKAKAALKRTTSDYFLLQVLLKFRTDKGRDPSSDTYEEDSELLLQIRN
DVLDSLGISPDLLPEDFVRYCFSEMAPVCAVVGGILAQEIVKALSQRDPP
HNNFFFFDGMKGNGIVECLGPK

• Vector Type: Bacterial
• DU Number: DU20243
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee