NEMO

• Plasmid: pCMV-TAG3b NEMO R319Q
• Parent Plasmid: pCMV tag3b
• Expressed: Myc-NEMO R319Q
• Genbank: AAH50612
• Tag: Myc
• Species: Human

Sequence of Insert

ATGGAGCAGAAACTCATCTCTGAAGAGGATCTGAGCCCGGGCGGATCCAA
TAGGCACCTCTGGAAGAGCCAACTGTGTGAGATGGTGCAGCCCAGTGGTG
GCCCGGCAGCAGATCAGGACGTACTGGGCGAAGAGTCTCCTCTGGGGAAG
CCAGCCATGCTGCACCTGCCTTCAGAACAGGGCGCTCCTGAGACCCTCCA
GCGCTGCCTGGAGGAGAATCAAGAGCTCCGAGATGCCATCCGGCAGAGCA
ACCAGATTCTGCGGGAGCGCTGCGAGGAGCTTCTGCATTTCCAAGCCAGC
CAGAGGGAGGAGAAGGAGTTCCTCATGTGCAAGTTCCAGGAGGCCAGGAA
ACTGGTGGAGAGACTCGGCCTGGAGAAGCTCGATCTGAAGAGGCAGAAGG
AGCAGGCTCTGCGGGAGGTGGAGCACCTGAAGAGATGCCAGCAGCAGATG
GCTGAGGACAAGGCCTCTGTGAAAGCCCAGGTGACGTCCTTGCTCGGGGA
GCTGCAGGAGAGCCAGAGTCGCTTGGAGGCTGCCACTAAGGAATGCCAGG
CTCTGGAGGGTCGGGCCCGGGCGGCCAGCGAGCAGGCGCGGCAGCTGGAG
AGTGAGCGCGAGGCGCTGCAGCAGCAGCACAGCGTGCAGGTGGACCAGCT
GCGCATGCAGGGCCAGAGCGTGGAGGCCGCGCTCCGCATGGAGCGCCAGG
CCGCCTCGGAGGAGAAGAGGAAGCTGGCCCAGTTGCAGGTGGCCTATCAC
CAGCTCTTCCAAGAATACGACAACCACATCAAGAGCAGCGTGGTGGGCAG
TGAGCGGAAGCGAGGAATGCAGCTGGAAGATCTCAAACAGCAGCTCCAGC
AGGCCGAGGAGGCCCTGGTGGCCAAACAGGAGGTGATCGATAAGCTGAAG
GAGGAGGCCGAGCAGCACAAGATTGTGATGGAGACCGTTCCGGTGCTGAA
GGCCCAGGCGGATATCTACAAGGCGGACTTCCAGGCTGAGAGGCAGGCCC
AGGAGAAGCTGGCCGAGAAGAAGGAGCTCCTGCAGGAGCAGCTGGAGCAG
CTGCAGAGGGAGTACAGCAAACTGAAGGCCAGCTGTCAGGAGTCGGCCAG
GATCGAGGACATGAGGAAGCGGCATGTCGAGGTCTCCCAGGCCCCCTTGC
CCCCCGCCCCTGCCTACCTCTCCTCTCCCCTGGCCCTGCCCAGCCAGAGG
AGGAGCCCCCCCGAGGAGCCACCTGACTTCTGCTGTCCCAAGTGCCAGTA
TCAGGCCCCTGATATGGACACCCTGCAGATACATGTCATGGAGTGCATTG
AGTAGGCGGCCGC

Amino Acid Sequence

MEQKLISEEDLSPGGSNRHLWKSQLCEMVQPSGGPAADQDVLGEESPLGK
PAMLHLPSEQGAPETLQRCLEENQELRDAIRQSNQILRERCEELLHFQAS
QREEKEFLMCKFQEARKLVERLGLEKLDLKRQKEQALREVEHLKRCQQQM
AEDKASVKAQVTSLLGELQESQSRLEAATKECQALEGRARAASEQARQLE
SEREALQQQHSVQVDQLRMQGQSVEAALRMERQAASEEKRKLAQLQVAYH
QLFQEYDNHIKSSVVGSERKRGMQLEDLKQQLQQAEEALVAKQEVIDKLK
EEAEQHKIVMETVPVLKAQADIYKADFQAERQAQEKLAEKKELLQEQLEQ
LQREYSKLKASCQESARIEDMRKRHVEVSQAPLPPAPAYLSSPLALPSQR
RSPPEEPPDFCCPKCQYQAPDMDTLQIHVMECIE*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU4833
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee