USP30

• Plasmid: pET156P USP30 57-end (lacks transmembrane domain)
• Parent Plasmid: pET15b 6His 3C
• Expressed: 6His-USP30 57-end
• Genbank: NM_032663.3 GI:164607166
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCACAGAAAGAAAGAAGCGTAGAAAAGGGCTTGTGCCTGGCCTTGT
TAATTTAGGGAACACCTGCTTCATGAACTCCCTGCTACAAGGCCTGTCTG
CCTGTCCTGCTTTCATCAGGTGGCTGGAAGAGTTCACCTCCCAGTACTCC
AGGGATCAGAAGGAGCCCCCCTCACACCAGTATTTATCCTTAACACTCTT
GCACCTTCTGAAAGCCTTGTCCTGCCAAGAAGTTACTGATGATGAGGTCT
TAGATGCAAGCTGCTTGTTGGATGTCTTAAGAATGTACAGATGGCAGATC
TCATCATTTGAAGAACAGGATGCTCACGAATTATTCCATGTCATTACCTC
GTCATTGGAAGATGAGCGAGACCGCCAGCCTCGGGTCACACATTTGTTTG
ATGTGCATTCCCTGGAGCAGCAGTCAGAAATAACTCCCAAACAAATTACC
TGCCGCACAAGAGGGTCACCTCACCCTACATCCAATCACTGGAAGTCTCA
ACATCCTTTTCATGGAAGACTCACTAGTAATATGGTCTGCAAACACTGTG
AACACCAGAGTCCTGTTCGATTTGATACCTTTGATAGCCTTTCACTAAGT
ATTCCAGCCGCCACATGGGGTCACCCATTGACCCTGGACCACTGCCTTCA
CCACTTCATCTCATCAGAATCAGTGCGGGATGTTGTGTGTGACAACTGTA
CAAAGATTGAAGCCAAGGGAACGTTGAACGGGGAAAAGGTGGAACACCAG
AGGACCACTTTTGTTAAACAGTTAAAACTAGGGAAGCTCCCTCAGTGTCT
CTGCATCCACCTACAGCGGCTGAGCTGGTCCAGCCACGGCACGCCTCTGA
AGCGGCATGAGCACGTGCAGTTCAATGAGTTCCTGATGATGGACATTTAC
AAGTACCACCTCCTTGGACATAAACCTAGTCAACACAACCCTAAACTGAA
CAAGAACCCAGGGCCTACACTGGAGCTGCAGGATGGGCCGGGAGCCCCCA
CACCAGTTCTGAATCAGCCAGGGGCCCCCAAAACACAGATTTTTATGAAT
GGCGCCTGCTCCCCATCTTTATTGCCAACGCTGTCAGCGCCGATGCCCTT
CCCTCTCCCAGTTGTTCCCGACTACAGCTCCTCCACATACCTCTTCCGGC
TGATGGCAGTTGTCGTCCACCATGGAGACATGCACTCTGGACACTTTGTC
ACTTACCGACGGTCCCCACCTTCTGCCAGGAACCCTCTCTCAACTAGCAA
TCAGTGGCTGTGGGTCTCCGATGACACTGTCCGCAAGGCCAGCCTGCAGG
AGGTCCTGTCCTCCAGCGCCTACCTGCTGTTCTACGAGCGCGTCCTTTCC
AGGATGCAGCACCAGAGCCAGGAGTGCAAGTCTGAAGAATGAGCGGCCGC

Amino Acid Sequence

MGSSHHHHHHSSGLEVLFQGPGSTERKKRRKGLVPGLVNLGNTCFMNSLL
QGLSACPAFIRWLEEFTSQYSRDQKEPPSHQYLSLTLLHLLKALSCQEVT
DDEVLDASCLLDVLRMYRWQISSFEEQDAHELFHVITSSLEDERDRQPRV
THLFDVHSLEQQSEITPKQITCRTRGSPHPTSNHWKSQHPFHGRLTSNMV
CKHCEHQSPVRFDTFDSLSLSIPAATWGHPLTLDHCLHHFISSESVRDVV
CDNCTKIEAKGTLNGEKVEHQRTTFVKQLKLGKLPQCLCIHLQRLSWSSH
GTPLKRHEHVQFNEFLMMDIYKYHLLGHKPSQHNPKLNKNPGPTLELQDG
PGAPTPVLNQPGAPKTQIFMNGACSPSLLPTLSAPMPFPLPVVPDYSSST
YLFRLMAVVVHHGDMHSGHFVTYRRSPPSARNPLSTSNQWLWVSDDTVRK
ASLQEVLSSSAYLLFYERVLSRMQHQSQECKSEE*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU36295
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee