USP49

• Plasmid: pET156P USP49
• Parent Plasmid: pET15b 6His 3C
• Expressed: 6His-USP49
• Genbank: NM_018561.3 GI:34147686
• Tag: 6His
• Species: Human

Sequence of Insert

AGATCTATGGATAGATGCAAACATGTAGGGCGGTTACGGCTCGCCCAGGA
CCACTCCATCCTGAACCCTCAGAAGTGGTGCTGCTTAGAGTGTGCCACCA
CCGAGTCCGTGTGGGCCTGCCTCAAGTGCTCCCACGTGGCCTGCGGCCGC
TATATTGAGGACCACGCCCTGAAACACTTTGAGGAGACGGGACACCCGCT
AGCCATGGAAGTCCGGGATCTCTACGTGTTCTGTTACCTGTGCAAGGACT
ACGTGCTCAATGATAACCCAGAGGGGGACCTGAAGCTGCTAAGAAGCTCC
CTCCTGGCGGTCCGGGGCCAGAAACAGGACACGCCGGTGAGACGTGGGCG
GACGCTGCGGTCCATGGCTTCGGGTGAGGACGTGGTCCTGCCGCAGCGCG
CTCCTCAGGGACAGCCGCAGATGCTCACGGCTCTGTGGTACCGGCGTCAG
CGCCTGCTGGCCAGGACGCTGCGGCTGTGGTTCGAGAAGAGCTCCCGGGG
CCAGGCGAAGCTGGAGCAGCGGCGGCAGGAGGAGGCCCTGGAGCGCAAGA
AGGAGGAGGCGCGGAGGCGGCGGCGCGAGGTGAAACGGCGGCTGCTGGAG
GAGCTGGCCAGCACCCCTCCGCGCAAGAGTGCACGGCTGCTCCTGCACAC
GCCCCGCGACGCGGGCCCGGCTGCCTCGCGCCCCGCCGCCCTCCCTACCT
CACGCAGAGTGCCCGCCGCCACACTCAAGCTGCGTCGCCAGCCGGCCATG
GCCCCAGGCGTCACGGGCCTGCGCAACCTGGGCAACACCTGCTACATGAA
CTCCATCCTCCAGGTGCTCAGCCACCTCCAGAAGTTCCGAGAATGTTTCC
TCAACCTTGACCCTTCCAAAACGGAACATCTGTTTCCCAAAGCCACCAAC
GGGAAGACTCAGCTTTCTGGCAAGCCAACCAACAGCTCGGCCACGGAGCT
GTCCTTGAGAAATGACAGGGCCGAGGCATGCGAGCGGGAGGGCTTCTGCT
GGAACGGCAGGGCCTCCATTAGTCGGAGTCTGGAGCTCATCCAGAACAAG
GAGCCGAGTTCAAAGCACATTTCCCTCTGCCGTGAACTGCACACCCTCTT
CCGAGTCATGTGGTCCGGGAAGTGGGCCCTAGTGTCGCCCTTCGCCATGC
TGCACTCAGTGTGGAGCCTGATCCCTGCCTTCCGCGGCTACGACCAACAG
GACGCGCAGGAATTTCTCTGCGAGCTGCTGCACAAGGTGCAGCAGGAACT
CGAGTCTGAGGGCACCACACGCCGGATCCTCATCCCCTTCTCCCAGAGGA
AGCTCACCAAACAGGTCTTAAAGGTGGTGAATACCATATTTCATGGGCAG
CTGCTCAGTCAGGTCACATGTATATCATGCAATTACAAATCCAATACCAT
TGAGCCCTTTTGGGACCTATCCCTGGAATTCCCTGAACGCTATCACTGCA
TAGAAAAGGGGTTTGTCCCTTTGAATCAAACAGAGTGCTTGCTCACTGAG
ATGCTGGCCAAATTCACAGAGACAGAGGCCCTGGAAGGGAGAATCTACGC
TTGTGACCAGTGTAACAGCAAACGACGAAAATCCAATCCCAAACCCCTTG
TTCTGAGTGAAGCTAGAAAGCAGTTAATGATCTACAGACTACCTCAGGTT
CTCCGGCTGCACCTTAAAAGATTCAGGTGGTCTGGCCGTAATCATCGAGA
GAAGATTGGGGTCCATGTCGTCTTTGACCAGGTATTAACCATGGAACCTT
ACTGCTGCAGGGACATGCTCTCCTCTCTTGACAAAGAGACCTTTGCCTAT
GATCTCTCCGCAGTGGTCATGCATCACGGGAAAGGGTTTGGCTCAGGACA
CTACACAGCCTATTGCTACAACACAGAGGGAGGTGCGTGCGCTTTACTCT
GTGGGGTGGGGGACACGGAAAGGGGTTGAGATCT

Amino Acid Sequence

MGSSHHHHHHSSGLEVLFQGPGSMDRCKHVGRLRLAQDHSILNPQKWCCL
ECATTESVWACLKCSHVACGRYIEDHALKHFEETGHPLAMEVRDLYVFCY
LCKDYVLNDNPEGDLKLLRSSLLAVRGQKQDTPVRRGRTLRSMASGEDVV
LPQRAPQGQPQMLTALWYRRQRLLARTLRLWFEKSSRGQAKLEQRRQEEA
LERKKEEARRRRREVKRRLLEELASTPPRKSARLLLHTPRDAGPAASRPA
ALPTSRRVPAATLKLRRQPAMAPGVTGLRNLGNTCYMNSILQVLSHLQKF
RECFLNLDPSKTEHLFPKATNGKTQLSGKPTNSSATELSLRNDRAEACER
EGFCWNGRASISRSLELIQNKEPSSKHISLCRELHTLFRVMWSGKWALVS
PFAMLHSVWSLIPAFRGYDQQDAQEFLCELLHKVQQELESEGTTRRILIP
FSQRKLTKQVLKVVNTIFHGQLLSQVTCISCNYKSNTIEPFWDLSLEFPE
RYHCIEKGFVPLNQTECLLTEMLAKFTETEALEGRIYACDQCNSKRRKSN
PKPLVLSEARKQLMIYRLPQVLRLHLKRFRWSGRNHREKIGVHVVFDQVL
TMEPYCCRDMLSSLDKETFAYDLSAVVMHHGKGFGSGHYTAYCYNTEGGA
CALLCGVGDTERG*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU36265
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee