ITCH

• Plasmid: pcDNA5-FRT/TO-HA ITCH C830A
• Parent Plasmid: pcDNA5 FRT/TO HA1
• Expressed: HA ITCH C830A
• Genbank: NM_031483
• Tag: HA
• Species: Human

Sequence of Insert

GGATCCATGTCTGACAGTGGATCACAACTTGGTTCAATGGGTAGCCTCACCATGAAATCACAGCTTCAGATCACTGTCATCTCAGCAAAACTTAAGGAAAATAAGAAGAATTGGTTTGGACCAAGTCCTTACGTAGAGGTCACAGTAGATGGACAGTCAAAGAAGACAGAAAAATGCAACAACACAAACAGTCCCAAGTGGAAGCAACCCCTTACAGTTATCGTTACCCCTGTGAGTAAATTACATTTTCGTGTGTGGAGTCACCAGACACTGAAATCTGATGTTTTGTTGGGAACTGCTGCATTA
GATATTTATGAAACATTAAAGTCAAACAATATGAAACTTGAAGAAGTAGT
TGTGACTTTGCAGCTTGGAGGTGACAAAGAGCCAACAGAGACAATAGGAG
ACTTGTCAATTTGTCTTGATGGGCTACAGTTAGAGTCTGAAGTTGTTACC
AATGGTGAAACTACATGTTCAGAAAGTGCTTCTCAGAATGATGATGGCTC
CAGATCCAAGGATGAAACAAGAGTGAGCACAAATGGATCAGATGACCCTG
AAGATGCAGGAGCTGGTGAAAATAGGAGAGTCAGTGGGAATAATTCTCCA
TCACTCTCAAATGGTGGTTTTAAACCTTCTAGACCTCCAAGACCTTCACG
ACCACCACCACCCACCCCACGTAGACCAGCATCTGTCAATGGTTCACCAT
CTGCCACTTCTGAAAGTGATGGGTCTAGTACAGGCTCTCTGCCGCCGACA
AATACAAATACAAATACATCTGAAGGAGCAACATCTGGATTAATAATTCC
TCTTACTATATCTGGAGGCTCAGGCCCTAGGCCATTAAATCCTGTAACTC
AAGCTCCCTTGCCACCTGGTTGGGAGCAGAGAGTGGACCAGCACGGGCGA
GTTTACTATGTAGATCATGTTGAGAAAAGAACAACATGGGATAGACCAGA
ACCTCTACCTCCTGGCTGGGAACGGCGGGTTGACAACATGGGACGTATTT
ATTATGTTGACCATTTCACAAGAACAACAACGTGGCAGAGGCCAACACTG
GAATCCGTCCGGAACTATGAACAATGGCAGCTACAGCGTAGTCAGCTTCA
AGGAGCAATGCAGCAGTTTAACCAGAGATTCATTTATGGGAATCAAGATT
TATTTGCTACATCACAAAGTAAAGAATTTGATCCTCTTGGTCCATTGCCA
CCTGGATGGGAGAAGAGAACAGACAGCAATGGCAGAGTATATTTCGTCAA
CCACAACACACGAATTACACAATGGGAAGACCCCAGAAGTCAAGGTCAAT
TAAATGAAAAGCCCTTACCTGAAGGTTGGGAAATGAGATTCACAGTGGAT
GGAATTCCATATTTTGTGGACCACAATAGAAGAACTACCACCTATATAGA
TCCCCGCACAGGAAAATCTGCCCTAGACAATGGACCTCAGATAGCCTATG
TTCGGGACTTCAAAGCAAAGGTTCAGTATTTCCGGTTCTGGTGTCAGCAA
CTGGCCATGCCACAGCACATAAAGATTACAGTGACAAGAAAAACATTGTT
TGAGGATTCCTTTCAACAGATAATGAGCTTCAGTCCCCAAGATCTGCGAA
GACGTTTGTGGGTGATTTTTCCAGGAGAAGAAGGTTTAGATTATGGAGGT
GTAGCAAGAGAATGGTTCTTTCTTTTGTCACATGAAGTGTTGAACCCAAT
GTATTGCCTGTTTGAATATGCAGGGAAGGATAACTACTGCTTGCAGATAA
ACCCCGCTTCTTACATCAATCCAGATCACCTGAAATATTTTCGTTTTATT
GGCAGATTCATTGCCATGGCTCTGTTCCATGGGAAATTCATAGACACGGG
TTTTTCTTTACCATTCTATAAGCGTATCTTGAACAAACCAGTTGGACTCA
AGGATTTAGAATCTATTGATCCAGAATTTTACAATTCTCTCATCTGGGTT
AAGGAAAACAATATTGAGGAATGTGATTTGGAAATGTACTTCTCCGTTGA
CAAAGAAATTCTAGGTGAAATTAAGAGTCATGATCTGAAACCTAATGGTG
GCAATATTCTTGTAACAGAAGAAAATAAAGAGGAATACATCAGAATGGTA
GCTGAGTGGAGGTTGTCTCGAGGTGTTGAAGAACAGACACAAGCTTTCTT
TGAAGGCTTTAATGAAATTCTTCCCCAGCAATATTTGCAATACTTTGATG
CAAAGGAATTAGAGGTCCTTTTATGTGGAATGCAAGAGATTGATTTGAAT
GACTGGCAAAGACATGCCATCTACCGTCATTATGCAAGGACCAGCAAACA
AATCATGTGGTTTTGGCAGTTTGTTAAAGAAATTGATAATGAGAAGAGAA
TGAGACTTCTGCAGTTTGTTACTGGAACCTGCCGATTGCCAGTAGGAGGA
TTTGCTGATCTCATGGGGAGCAATGGACCACAGAAATTCTGCATTGAAAA
AGTTGGGAAAGAAAATTGGCTACCCAGAAGTCATACCGCTTTTAATCGCC
TGGACCTGCCACCATACAAGAGCTATGAGCAACTGAAGGAAAAGCTGTTG
TTTGCCATAGAAGAAACAGAAGGATTTGGACAAGAGTAAGCGGCCGC

Amino Acid Sequence

MYPYDVPDYAGSMSDSGSQLGSMGSLTMKSQLQITVISAKLKENKKNWFG
PSPYVEVTVDGQSKKTEKCNNTNSPKWKQPLTVIVTPVSKLHFRVWSHQT
LKSDVLLGTAALDIYETLKSNNMKLEEVVVTLQLGGDKEPTETIGDLSIC
LDGLQLESEVVTNGETTCSESASQNDDGSRSKDETRVSTNGSDDPEDAGA
GENRRVSGNNSPSLSNGGFKPSRPPRPSRPPPPTPRRPASVNGSPSATSE
SDGSSTGSLPPTNTNTNTSEGATSGLIIPLTISGGSGPRPLNPVTQAPLP
PGWEQRVDQHGRVYYVDHVEKRTTWDRPEPLPPGWERRVDNMGRIYYVDH
FTRTTTWQRPTLESVRNYEQWQLQRSQLQGAMQQFNQRFIYGNQDLFATS
QSKEFDPLGPLPPGWEKRTDSNGRVYFVNHNTRITQWEDPRSQGQLNEKP
LPEGWEMRFTVDGIPYFVDHNRRTTTYIDPRTGKSALDNGPQIAYVRDFK
AKVQYFRFWCQQLAMPQHIKITVTRKTLFEDSFQQIMSFSPQDLRRRLWV
IFPGEEGLDYGGVAREWFFLLSHEVLNPMYCLFEYAGKDNYCLQINPASY
INPDHLKYFRFIGRFIAMALFHGKFIDTGFSLPFYKRILNKPVGLKDLES
IDPEFYNSLIWVKENNIEECDLEMYFSVDKEILGEIKSHDLKPNGGNILV
TEENKEEYIRMVAEWRLSRGVEEQTQAFFEGFNEILPQQYLQYFDAKELE
VLLCGMQEIDLNDWQRHAIYRHYARTSKQIMWFWQFVKEIDNEKRMRLLQ
FVTGTCRLPVGGFADLMGSNGPQKFCIEKVGKENWLPRSHTAFNRLDLPP
YKSYEQLKEKLLFAIEETEGFGQE*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU31375
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee