USP8

• Plasmid: pGEX6P-1-USP8 new
• Parent Plasmid: pGEX6P1
• Expressed: GST USP8
• Genbank: NP_005145.3
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGCCTGCTGTGGCTTCAGTTCCTAAAGAACTCTACCTCAGTTC
TTCACTAAAAGACCTTAATAAGAAGACAGAAGTTAAACCAGAGAAAATAA
GCACTAAGAGTTATGTGCACAGTGCCCTGAAGATCTTTAAGACAGCAGAA
GAATGCAGATTAGATCGTGATGAGGAAAGGGCCTATGTACTATATATGAA
ATACGTGACTGTTTATAATCTTATCAAAAAAAGACCTGATTTCAAGCAAC
AGCAGGATTATTTCCATTCAATACTTGGACCTGGAAACATCAAAAAAGCT
GTCGAAGAAGCTGAAAGACTCTCTGAAAGCCTTAAATTAAGATATGAAGA
AGCTGAAGTCCGGAAAAAACTTGAGGAAAAAGACAGGCAGGAGGAAGCAC
AGCGGCTACAACAAAAAAGGCAGGAAACAGGAAGAGAGGATGGTGGCACA
TTGGCTAAAGGCTCTTTGGAGAATGTTTTGGATTCCAAAGACAAAACCCA
AAAGAGCAATGGTGAAAAGAATGAAAAATGTGAGACCAAAGAGAAAGGAG
CAATCACAGCAAAGGAACTATACACAATGATGACGGATAAAAACATCAGC
TTGATTATAATGGATGCTCGAAGAATGCAGGATTATCAGGATTCCTGTAT
TTTACATTCTCTCAGTGTTCCTGAAGAAGCCATCAGTCCAGGAGTCACTG
CTAGTTGGATTGAAGCACACCTGCCAGATGATTCTAAAGACACATGGAAG
AAGAGGGGGAATGTGGAGTATGTGGTACTTCTTGACTGGTTTAGTTCTGC
CAAAGATTTACAGATTGGAACAACTCTCCGGAGTCTGAAAGATGCACTTT
TCAAGTGGGAAAGTAAAACTGTCCTGCGCAATGAGCCTTTGGTTTTAGAG
GGAGGCTATGAAAACTGGCTCCTTTGTTATCCCCAGTATACAACAAATGC
TAAGGTCACTCCACCCCCACGACGCCAGAATGAAGAGGTGTCTATCTCAT
TGGATTTTACTTATCCCTCATTGGAAGAATCAATTCCTTCTAAACCTGCT
GCCCAGACGCCACCTGCATCTATAGAAGTAGATGAAAATATAGAATTGAT
AAGTGGTCAAAATGAGAGAATGGGACCACTGAATATATCAACTCCAGTTG
AACCAGTTGCTGCTTCTAAATCTGATGTTTCACCCATAATTCAGCCAGTG
CCTAGTATAAAGAATGTTCCACAGATTGATCGTACTAAAAAACCAGCAGT
CAAATTGCCTGAAGAGCATAGAATAAAATCTGAAAGTACAAACCATGAGC
AACAATCTCCTCAGAGTGGAAAAGTTATTCCTGATCGTTCCACCAAGCCA
GTAGTTTTTTCTCCAACTCTCATGTTAACAGATGAAGAAAAGGCTCGTAT
TCATGCAGAAACTGCTCTTCTAATGGAAAAAAACAAACAAGAAAAAGAAC
TTCGGGAAAGGCAGCAAGAGGAACAGAAAGAGAAACTGAGGAAGGAAGAA
CAAGAACAAAAAGCCAAAAAGAAACAAGAAGCTGAAGAAAATGAAATTAC
AGAGAAGCAACAAAAAGCAAAAGAAGAAATGGAGAAGAAAGAAAGTGAAC
AGGCCAAGAAAGAAGATAAAGAAACATCAGCAAAGAGGGGCAAAGAAATA
ACAGGAGTAAAAAGACAAAGTAAAAGTGAACATGAAACTTCTGATGCCAA
GAAATCTGTAGAAGATAGGGGGAAAAGGTGTCCAACCCCAGAAATACAGA
AAAAGTCAACAGGAGATGTGCCCCATACATCTGTGACAGGGGATTCAGGT
TCAGGCAAGCCATTTAAGATTAAAGGACAACCAGAAAGTGGAATTCTAAG
GACAGGAACTTTTAGAGAGGATACAGACGATACCGAAAGAAATAAAGCTC
AACGAGAACCTTTGACAAGAGCACGAAGTGAAGAAATGGGGAGGATCGTA
CCAGGACTGCCTTCAGGCTGGGCCAAGTTTCTTGACCCAATCACTGGAAC
CTTTCGTTATTATCATTCACCCACCAACACTGTTCATATGTACCCACCGG
AAATGGCTCCTTCATCTGCACCTCCTTCCACCCCTCCAACTCATAAAGCC
AAGCCACAGATTCCTGCTGAGCGGGATAGGGAACCTTCCAAACTGAAGCG
CTCCTACTCCTCCCCAGATATAACCCAGGCTATTCAAGAGGAAGAGAAGA
GGAAGCCAACAGTAACTCCAACAGTTAATCGGGAAAACAAGCCAACATGT
TATCCTAAAGCTGAGATCTCAAGGCTTTCTGCTTCTCAGATTCGGAACCT
CAATCCTGTTTTTGGAGGTTCTGGACCAGCTCTTACTGGACTTCGTAACT
TAGGAAATACTTGTTATATGAACTCAATATTGCAGTGCCTATGTAACGCT
CCACATTTGGCTGATTATTTCAACCGAAACTGTTATCAGGATGATATTAA
CAGGTCAAATTTGTTGGGGCATAAAGGTGAAGTGGCAGAAGAATTTGGTA
TAATCATGAAAGCCCTGTGGACAGGACAGTATAGATATATCAGTCCAAAG
GACTTTAAAATCACCATTGGGAAGATCAATGACCAGTTTGCAGGATACAG
TCAGCAAGATTCACAAGAATTGCTTCTGTTCCTAATGGATGGTCTCCATG
AAGATCTAAATAAAGCTGATAATCGGAAGAGATATAAAGAAGAAAATAAT
GATCATCTCGATGACTTTAAAGCTGCAGAACATGCCTGGCAGAAACACAA
GCAGCTCAATGAGTCTATTATTGTTGCACTTTTTCAGGGTCAATTCAAAT
CTACAGTACAGTGCCTCACATGTCACAAAAAGTCTAGGaCATTTGAGGCC
TTCATGTATTTGTCTCTACCACTAGCATCCACAAGTAAATGTACATTACA
GGATTGCCTTAGATTATTTTCCAAAGAAGAAAAACTCACAGATAACAACA
GATTTTACTGCAGTCATTGCAGAGCTCGACGGGATTCTCTAAAAAAGATA
GAAATCTGGAAGTTACCACCTGTGCTTTTAGTGCATCTGAAACGTTTTTC
CTACGATGGCAGGTGGAAACAAAAATTACAGACATCTGTGGACTTCCCGT
TAGAAAATCTTGACTTGTCACAGTATGTTATTGGTCCAAAGAACAATTTG
AAGAAATATAATTTGTTTTCTGTTTCAAATCACTACGGTGGGCTGGATGG
AGGCCACTACACAGCCTATTGTAAAAATGCAGCAAGACAACGGTGGTTTA
AGTTTGATGATCATGAAGTTTCTGATATCTCCGTTTCTTCTGTGAAATCT
TCAGCAGCTTATATCCTCTTTTATACTTCATTGGGACCACGAGTAACTGA
TGTAGCCACATAAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMPAVASVPKELYLSSSLKD
LNKKTEVKPEKISTKSYVHSALKIFKTAEECRLDRDEERAYVLYMKYVTV
YNLIKKRPDFKQQQDYFHSILGPGNIKKAVEEAERLSESLKLRYEEAEVR
KKLEEKDRQEEAQRLQQKRQETGREDGGTLAKGSLENVLDSKDKTQKSNG
EKNEKCETKEKGAITAKELYTMMTDKNISLIIMDARRMQDYQDSCILHSL
SVPEEAISPGVTASWIEAHLPDDSKDTWKKRGNVEYVVLLDWFSSAKDLQ
IGTTLRSLKDALFKWESKTVLRNEPLVLEGGYENWLLCYPQYTTNAKVTP
PPRRQNEEVSISLDFTYPSLEESIPSKPAAQTPPASIEVDENIELISGQN
ERMGPLNISTPVEPVAASKSDVSPIIQPVPSIKNVPQIDRTKKPAVKLPE
EHRIKSESTNHEQQSPQSGKVIPDRSTKPVVFSPTLMLTDEEKARIHAET
ALLMEKNKQEKELRERQQEEQKEKLRKEEQEQKAKKKQEAEENEITEKQQ
KAKEEMEKKESEQAKKEDKETSAKRGKEITGVKRQSKSEHETSDAKKSVE
DRGKRCPTPEIQKKSTGDVPHTSVTGDSGSGKPFKIKGQPESGILRTGTF
REDTDDTERNKAQREPLTRARSEEMGRIVPGLPSGWAKFLDPITGTFRYY
HSPTNTVHMYPPEMAPSSAPPSTPPTHKAKPQIPAERDREPSKLKRSYSS
PDITQAIQEEEKRKPTVTPTVNRENKPTCYPKAEISRLSASQIRNLNPVF
GGSGPALTGLRNLGNTCYMNSILQCLCNAPHLADYFNRNCYQDDINRSNL
LGHKGEVAEEFGIIMKALWTGQYRYISPKDFKITIGKINDQFAGYSQQDS
QELLLFLMDGLHEDLNKADNRKRYKEENNDHLDDFKAAEHAWQKHKQLNE
SIIVALFQGQFKSTVQCLTCHKKSRTFEAFMYLSLPLASTSKCTLQDCLR
LFSKEEKLTDNNRFYCSHCRARRDSLKKIEIWKLPPVLLVHLKRFSYDGR
WKQKLQTSVDFPLENLDLSQYVIGPKNNLKKYNLFSVSNHYGGLDGGHYT
AYCKNAARQRWFKFDDHEVSDISVSSVKSSAAYILFYTSLGPRVTDVAT*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU35686
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee