USP7

• Plasmid: pCMV-FLAG-1 USP7 50-550
• Parent Plasmid: pCMV5 FLAG1
• Expressed: FLAG USP7 50-550
• Genbank: NP_003461.2
• Tag: FLAG
• Species: Human

Sequence of Insert

GGATCCGATGGACACAACACCGCGGAGGAGGACATGGAGGATGACACCAG
TTGGCGCTCCGAGGCAACCTTTCAGTTCACTGTGGAGCGCTTCAGCAGAC
TGAGTGAGTCGGTCCTTAGCCCTCCGTGTTTTGTGCGAAATCTGCCATGG
AAGATTATGGTGATGCCACGCTTTTATCCAGACAGACCACACCAAAAAAG
CGTAGGATTCTTTCTCCAGTGCAATGCTGAATCTGATTCCACGTCATGGT
CTTGCCATGCACAAGCAGTGCTGAAGATAATAAATTACAGAGATGATGAA
AAGTCGTTCAGTCGTCGTATTAGTCATTTGTTCTTCCATAAAGAAAATGA
TTGGGGATTTTCCAATTTTATGGCCTGGAGTGAAGTGACCGATCCTGAGA
AAGGATTTATAGATGATGACAAAGTTACCTTTGAAGTCTTTGTACAGGCG
GATGCTCCCCATGGAGTTGCGTGGGATTCAAAGAAGCACACAGGCTACGT
CGGCTTAAAGAATCAGGGAGCGACTTGTTACATGAACAGCCTGCTACAGA
CGTTATTTTTCACGAATCAGCTACGAAAGGCTGTGTACATGATGCCAACC
GAGGGGGATGATTCGTCTAAAAGCGTCCCTTTAGCATTACAAAGAGTGTT
CTATGAATTACAGCATAGTGATAAACCTGTAGGAACAAAAAAGTTAACAA
AGTCATTTGGGTGGGAAACTTTAGATAGCTTCATGCAACATGATGTTCAG
GAGCTTTGTCGAGTGTTGCTCGATAATGTGGAAAATAAGATGAAAGGCAC
CTGTGTAGAGGGCACCATACCCAAATTATTCCGCGGCAAAATGGTGTCCT
ATATCCAGTGTAAAGAAGTAGACTATCGGTCTGATAGAAGAGAAGATTAT
TATGATATCCAGCTAAGTATCAAAGGAAAGAAAAATATATTTGAATCATT
TGTGGATTATGTGGCAGTAGAACAGCTCGATGGGGACAATAAATACGACG
CTGGGGAACATGGCTTACAGGAAGCAGAGAAAGGTGTGAAATTCCTAACA
TTGCCACCAGTGTTACATCTACAACTGATGAGATTTATGTATGACCCTCA
GACGGACCAAAATATCAAGATCAATGATAGGTTTGAATTCCCAGAGCAGT
TACCACTTGATGAATTTTTGCAAAAAACAGATCCTAAGGACCCTGCAAAT
TATATTCTTCATGCAGTCCTGGTTCATAGTGGAGATAATCATGGTGGACA
TTATGTGGTTTATCTAAACCCCAAAGGGGATGGCAAATGGTGTAAATTTG
ATGACGACGTGGTGTCAAGGTGTACTAAAGAGGAAGCAATTGAGCACAAT
TATGGGGGTCACGATGACGACCTGTCTGTTCGACACTGCACTAATGCTTA
CATGTTAGTCTACATCAGGGAATCAAAACTGAGTGAAGTTTTACAGGCGG
TCACCGACCATGATATTCCTCAGCAGTTGGTGGAGCGATTACAAGAAGAG
AAAAGGATCTAAGCGGCCGC

Amino Acid Sequence

MDYKDDDDKGSDGHNTAEEDMEDDTSWRSEATFQFTVERFSRLSESVLSP
PCFVRNLPWKIMVMPRFYPDRPHQKSVGFFLQCNAESDSTSWSCHAQAVL
KIINYRDDEKSFSRRISHLFFHKENDWGFSNFMAWSEVTDPEKGFIDDDK
VTFEVFVQADAPHGVAWDSKKHTGYVGLKNQGATCYMNSLLQTLFFTNQL
RKAVYMMPTEGDDSSKSVPLALQRVFYELQHSDKPVGTKKLTKSFGWETL
DSFMQHDVQELCRVLLDNVENKMKGTCVEGTIPKLFRGKMVSYIQCKEVD
YRSDRREDYYDIQLSIKGKKNIFESFVDYVAVEQLDGDNKYDAGEHGLQE
AEKGVKFLTLPPVLHLQLMRFMYDPQTDQNIKINDRFEFPEQLPLDEFLQ
KTDPKDPANYILHAVLVHSGDNHGGHYVVYLNPKGDGKWCKFDDDVVSRC
TKEEAIEHNYGGHDDDLSVRHCTNAYMLVYIRESKLSEVLQAVTDHDIPQ
QLVERLQEEKRI*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU32699
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee