SMURF2

• Plasmid: pEBG6P-SMURF2 C716A
• Parent Plasmid: pEBG6P
• Expressed: GST Smurf2 C716A
• Tag: GST
• Species: Human

Sequence of Insert

ATGTCTAACCCCGGAGGCCGGAGGAACGGGCCCGTCAAGCTGCGCCTGAC
AGTACTCTGTGCAAAAAACCTGGTGAAAAAGGATTTTTTCCGACTTCCTG
ATCCATTTGCTAAGGTGGTGGTTGATGGATCTGGGCAATGCCATTCTACA
GATACTGTGAAGAATACGCTTGATCCAAAGTGGAATCAGCATTATGACCT
GTATATTGGAAAGTCTGATTCAGTTACGATCAGTGTATGGAATCACAAGA
AGATCCATAAGAAACAAGGTGCTGGATTTCTCGGTTGTGTTCGTCTTCTT
TCCAATGCCATCAACCGCCTCAAAGACACTGGTTATCAGAGGTTGGATTT
ATGCAAACTCGGGCCAAATGACAATGATACAGTTAGAGGACAGATAGTAG
TAAGTCTTCAGTCCAGAGACCGAATAGGCACAGGAGGACAAGTTGTGGAC
TGCAGTCGTTTATTTGATAACGATTTACCAGACGGCTGGGAAGAAAGGAG
AACCGCCTCTGGAAGAATCCAGTATCTAAACCATATAACAAGAACTACGC
AATGGGAGCGCCCAACACGACCGGCATCCGAATATTCTAGCCCTGGCAGA
CCTCTTAGCTGCTTTGTTGATGAGAACACTCCAATTAGTGGAACAAATGG
TGCAACATGTGGACAGTCTTCAGATCCCAGGCTGGCAGAGAGGAGAGTCA
GGTCACAACGACATAGAAATTACATGAGCAGAACACATTTACATACTCCT
CCAGACCTACCAGAAGGCTATGAACAGAGGACAACGCAACAAGGCCAGGT
GTATTTCTTACATACACAGACTGGTGTGAGCACATGGCATGATCCAAGAG
TGCCCAGGGATCTTAGCAACATCAATTGTGAAGAGCTTGGTCCATTGCCT
CCTGGATGGGAGATCCGTAATACGGCAACAGGCAGAGTTTATTTCGTTGA
CCATAACAACAGAACAACACAATTTACAGATCCTCGGCTGTCTGCTAACT
TGCATTTAGTTTTAAATCGGCAGAACCAATTGAAAGACCAACAGCAACAG
CAAGTGGTATCGTTATGTCCTGATGACACAGAATGCCTGACAGTCCCAAG
GTACAAGCGAGACCTGGTTCAGAAACTAAAAATTTTGCGGCAAGAACTTT
CCCAACAACAGCCTCAGGCAGGTCATTGCCGCATTGAGGTTTCCAGGGAA
GAGATTTTTGAGGAATCATATCGACAGGTCATGAAAATGAGACCAAAAGA
TCTCTGGAAGCGATTAATGATAAAATTTCGTGGAGAAGAAGGCCTTGACT
ATGGAGGCGTTGCCAGGGAATGGTTGTATCTCTTGTCACATGAAATGTTG
AATCCATACTATGGCCTCTTCCAGTATTCAAGAGATGATATTTATACATT
GCAGATCAATCCTGATTCTGCAGTTAATCCGGAACATTTATCCTATTTCC
ACTTTGTTGGACGAATAATGGGAATGGCTGTGTTTCATGGACATTATATT
GATGGTGGTTTCACATTGCCTTTTTATAAGCAATTGCTTGGGAAGTCAAT
TACCTTGGATGACATGGAGTTAGTAGATCCGGATCTTCACAACAGTTTAG
TGTGGATACTTGAGAATGATATTACAGGTGTTTTGGACCATACCTTCTGT
GTTGAACATAATGCATATGGTGAAATTATTCAGCATGAACTTAAACCAAA
TGGCAAAAGTATCCCTGTTAATGAAGAAAATAAAAAAGAATATGTCAGGC
TCTATGTGAACTGGAGATTTTTACGAGGCATTGAGGCTCAATTCTTGGCT
CTGCAGAAAGGATTTAATGAAGTAATTCCACAACATCTGCTGAAGACATT
TGATGAGAAGGAGTTAGAGCTCATTATTTGTGGACTTGGAAAGATAGATG
TTAATGACTGGAAGGTAAACACCCGGTTAAAACACTGTACACCAGACAGC
AACATTGTCAAATGGTTCTGGAAAGCTGTGGAGTTTTTTGATGAAGAGCG
ACGAGCAAGATTGCTTCAGTTTGTGACAGGATCCTCTCGAGTGCCTCTGC
AGGGCTTCAAAGCATTGCAAGGTGCTGCAGGCCCGAGACTCTTTACCATA
CACCAGATTGATGCCTGCACTAACAACCTGCCGAAAGCCCACACTGCCTT
CAATCGAATAGACATTCCACCCTATGAAAGCTATGAAAAGCTATATGAAA
AGCTGCTAACAGCCATTGAAGAAACATGTGGATTTGCTGTGGAATGA

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSTSGTIDAAAAAAMSNPGGRRNGPVKLRLTVLCAKNLVKKDFFRLPDPFAKVVVDGSGQCHSTDTVKNTLDPKWNQHYDLYIGKSDSVTISVWNHKKIHKKQGAGFLGCVRLLSNAINRLKDTGYQRLDLCKLGPNDNDTVRGQIVVSLQSRDRIGTGGQVVDCSRLFDNDLPDGWEERRTASGRIQYLNHITRTTQWERPTRPASEYSSPGRPLSCFVDENTPISGTNGATCGQSSDPRLAERRVRSQRHRNYMSRTHLHTPPDLPEGYEQRTTQQGQVYFLHTQTGVSTWHDPRVPRDLSNINCEELGPLPPGWEIRNTATGRVYFVDHNNRTTQFTDPRLSANLHLVLNRQNQLKDQQQQQVVSLCPDDTECLTVPRYKRDLVQKLKILRQELSQQQPQAGHCRIEVSREEIFEESYRQVMKMRPKDLWKRLMIKFRGEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSRDDIYTLQINPDSAVNPEHLSYFHFVGRIMGMAVFHGHYIDGGFTLPFYKQLLGKSITLDDMELVDPDLHNSLVWILENDITGVLDHTFCVEHNAYGEIIQHELKPNGKSIPVNEENKKEYVRLYVNWRFLRGIEAQFLALQKGFNEVIPQHLLKTFDEKELELIICGLGKIDVNDWKVNTRLKHCTPDSNIVKWFWKAVEFFDEERRARLLQFVTGSSRVPLQGFKALQGAAGPRLFTIHQIDACTNNLPKAHTAFNRIDIPPYESYEKLYEKLLTAIEETCGFAVE*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU32614
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee