UBE4A

• Synonyms: E4; UFD2; UBOX2; NEDHMS
• Plasmid: pCMV5 FLAG UBE4A
• Parent Plasmid: pCMV5 FLAG1
• Expressed: FLAG UBE4A
• Genbank: NM_001204077.2
• Tag: FLAG
• Species: Human

Sequence of Insert

GCGGCCGCGATGACAGACCAGGAGAATAACAACAACATCTCAAGTAACCCCTTTGCTGCTCTTTTTGGCTCCCTGGCTGATGCCAAACAGTTTGCGGCAATCCAAAAAGAGCAGCTGAAGCAACAATCTGATGAACTCCCAGCTAGCCCAGATGACTCGGATAATAGCGTGTCAGAGAGCCTGGATGAATTCGATTACTCTGTGGCTGAGATTAGCCGCTCATTCCGATCACAGCAGGAAATATGTGAGCAACTCAACATCAATCACATGATCCAAAGGATCTTCCTTATTACTCTGGACAACAGTGATCCCAGCTTGAAAAGCGGGAATGGCATCCCTAGCCGTTGTGTGTATTTGGAAGAAATGGCAGTAGAGCTAGAAGATCAAGACTGGCTTGATATGAGCAATGTTGAGCAGGCCCTCTTCGCTCGCTTATTACTTCAAGATCCAGGCAACCACTTAATTAACATGACTTCTTCTACAACGCTAAATCTCTCTGCTGATCGAGATGCAGGAGAGAGGCACATTTTTTGTTACCTTTACTCCTGCTTCCAGAGAGCCAAGGAAGAGATTACCAAAGTTCCAGAGAACCTGCTACCCTTTGCAGTGCAGTGCAGAAACCTCACTGTGTCCAATACCCGAACAGTTCTTCTCACCCCAGAGATCTATGTTGACCAAAACATCCATGAGCAACTGGTAGATTTGATGTTAGAAGCCATCCAGGGAGCCCATTTTGAAGATGTAACTGAGTTTCTGGAAGAGGTCATTGAAGCCTTGATATTGGATGAGGAAGTTAGAACATTTCCAGAAGTCATGATTCCAGTGTTTGATATTTTATTGGGCCGAATAAAAGATCTAGAGCTCTGTCAGATCCTTTTGTATGCATATCTGGATATTCTTCTCTATTTCACTAGGCAAAAAGATATGGCAAAGGTTTTTGTAGAATACATTCAGCCCAAGGACCCTACCAATGGGCAAATGTACCAGAAGACCTTGCTGGGAGTAATTCTGAGTATCTCCTGCTTATTAAAGACTCCGGGTGTTGTAGAAAATCATGGCTACTTTTTGAATCCATCTCGTTCCAGCCCCCAGGAGATCAAAGTACAGGAGGCCAACATCCATCAGTTCATGGCTCAGTTCCACGAAAAGATCTACCAGATGCTGAAGAACTTACTCCAGCTCTCTCCAGAAACCAAACACTGTATCTTGTCCTGGCTTGGAAACTGTTTGCATGCAAATGCAGGCCGCACCAAGATTTGGGCCAATCAGATGCCAGAAATCTTTTTCCAAATGTATGCCTCAGATGCTTTCTTTCTGAATCTGGGTGCTGCTCTCCTGAAGCTATGCCAGCCATTTTGCAAACCCAGATCCTCTCGGCTCCTCACCTTTAATCCCACATACTGTGCCCTCAAGGAGTTGAATGATGAAGAACGAAAAATTAAAAATGTACACATGAGAGGTTTGGACAAAGAAACCTGTTTGATCCCAGCTGTGCAGGAGCCGAAGTTTCCACAGAACTACAACCTTGTAACAGAGAACCTTGCTCTGACAGAGTACACCTTGTACTTGGGATTTCACAGGTTGCATGATCAGATGGTAAAAATCAACCAAAATCTGCATCGGCTGCAGGTTGCCTGGCGGGATGCTCAGCAAAGTTCTAGCCCTGCTGCTGACAATCTTCGTGAGCAGTTTGAACGACTGATGACCATCTATCTTTCTACCAAGACTGCCATGACAGAGCCACAAATGCTACAAAACTGCCTAAACTTGCAGGTGTCCATGGCTGTTCTACTGGTTCAACTGGCCATAGGCAATGAGGGCTCACAGCCAATAGAGCTAACCTTTCCTTTGCCAGATGGCTACAGCTCTTTGGCTTATGTGCCAGAATTTTTTGCAGATAACCTGGGTGATTTTCTCATTTTTCTCCGCCGCTTTGCCGATGACATTTTGGAGACATCAGCAGATTCCCTGGAGCATGTCCTTCACTTTATCACCATTTTCACTGGAAGCATAGAAAGAATGAAGAATCCCCACCTGAGGGCCAAACTAGCAGAGGTGTTGGAAGCAGTGATGCCCCACCTGGATCAGACCCCAAATCCCTTGGTATCCAGTGTGTTCCACCGGAAACGTGTGTTCTGCAACTTTCAGTATGCACCCCAACTTGCAGAGGCTCTAATCAAGGTTTTTGTGGACATCGAATTTACAGGAGACCCCCATCAATTTGAACAGAAGTTTAATTACCGCCGTCCCATGTATCCTATCCTAAGATACATGTGGGGGACAGATACCTATCGGGAGAGCATTAAGGATTTGGCTGACTATGCCTCTAAGAATTTAGAAGCCATGAATCCCCCACTTTTCCTCCGCTTTCTTAACCTGCTAATGAATGATGCCATCTTCCTTTTGGATGAAGCCATACAGTATTTGAGCAAGATAAAGATTCAGCAAATTGAGAAGGATCGAGGTGAATGGGATAGTCTGACTCCAGAAGCCCGCCGAGAAAAGGAGGCTGGCCTACAGATGTTTGGACAGCTGGCACGTTTCCATAACATCATGTCCAATGAAACAATCGGTACCCTTGCCTTTCTCACATCAGAGATCAAGTCACTCTTTGTGCATCCCTTCCTGGCTGAGCGCATCATCTCCATGTTGAACTACTTCCTGCAACACCTGGTTGGCCCCAAGATGGGTGCCTTAAAAGTCAAGGACTTCAGCGAATTTGACTTCAAACCCCAGCAGCTTGTATCAGATATCTGCACTATCTACTTAAATCTTGGGGATGAGGAGAATTTCTGTGCCACTGTGCCCAAGGATGGACGTTCCTATTCCCCAACTCTCTTTGCACAGACAGTTCGAGTCTTGAAGAAAATAAATAAGCCTGGGAATATGATTATGGCTTTCAGCAACTTGGCAGAGAGAATCAAGTCTCTTGCAGACCTCCAACAACAGGAAGAGGAAACCTATGCAGATGCCTGTGATGAGTTCCTGGATCCCATTATGAGCACACTGATGTGTGACCCTGTGGTGCTGCCATCTTCCAGAGTCACTGTGGATAGATCCACCATTGCAAGACATTTGCTCAGTGACCAAACAGATCCCTTTAACCGTAGTCCCCTCACCATGGACCAGATCCGGCCAAACACAGAACTAAAAGAAAAAATCCAACGGTGGCTTGCAGAGAGGAAACAACAAAAGGAGCAACTTGAATAGGCGGCCGC

Amino Acid Sequence

MDYKDDDDKGIPGIPGSTRAAAMTDQENNNNISSNPFAALFGSLADAKQFAAIQKEQLKQQSDELPASPDDSDNSVSESLDEFDYSVAEISRSFRSQQEI
CEQLNINHMIQRIFLITLDNSDPSLKSGNGIPSRCVYLEEMAVELEDQDWLDMSNVEQALFARLLLQDPGNHLINMTSSTTLNLSADRDAGERHIFCYLY
SCFQRAKEEITKVPENLLPFAVQCRNLTVSNTRTVLLTPEIYVDQNIHEQLVDLMLEAIQGAHFEDVTEFLEEVIEALILDEEVRTFPEVMIPVFDILLG
RIKDLELCQILLYAYLDILLYFTRQKDMAKVFVEYIQPKDPTNGQMYQKTLLGVILSISCLLKTPGVVENHGYFLNPSRSSPQEIKVQEANIHQFMAQFH
EKIYQMLKNLLQLSPETKHCILSWLGNCLHANAGRTKIWANQMPEIFFQMYASDAFFLNLGAALLKLCQPFCKPRSSRLLTFNPTYCALKELNDEERKIK
NVHMRGLDKETCLIPAVQEPKFPQNYNLVTENLALTEYTLYLGFHRLHDQMVKINQNLHRLQVAWRDAQQSSSPAADNLREQFERLMTIYLSTKTAMTEP
QMLQNCLNLQVSMAVLLVQLAIGNEGSQPIELTFPLPDGYSSLAYVPEFFADNLGDFLIFLRRFADDILETSADSLEHVLHFITIFTGSIERMKNPHLRA
KLAEVLEAVMPHLDQTPNPLVSSVFHRKRVFCNFQYAPQLAEALIKVFVDIEFTGDPHQFEQKFNYRRPMYPILRYMWGTDTYRESIKDLADYASKNLEA
MNPPLFLRFLNLLMNDAIFLLDEAIQYLSKIKIQQIEKDRGEWDSLTPEARREKEAGLQMFGQLARFHNIMSNETIGTLAFLTSEIKSLFVHPFLAERII
SMLNYFLQHLVGPKMGALKVKDFSEFDFKPQQLVSDICTIYLNLGDEENFCATVPKDGRSYSPTLFAQTVRVLKKINKPGNMIMAFSNLAERIKSLADLQ
QQEEETYADACDEFLDPIMSTLMCDPVVLPSSRVTVDRSTIARHLLSDQTDPFNRSPLTMDQIRPNTELKEKIQRWLAERKQQKEQLE*

• Vector Type: Mammalian
• Antibiotic: Amp
• Full Snapgene Map: pCMV5 FLAG UBE4A628.04 KBSnapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU15574
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee