USP37

• Plasmid: pcDNA5-FRT/TO-FLAG USP37 M73-end
• Parent Plasmid: pcDNA5 FRT/TO FLAG2
• Expressed: FLAG USP37 M73-end
• Genbank: NM_020935.2
• Tag: FLAG
• Species: Human

Sequence of Insert

GCGGCCGCGATGTTAACTCTGCAAGATAACAGCTTCTTGTCTATTGACAA
AGTACCAAGTAAGGATGCAGAGGAAATGAGGTTGTTTCTAGATGCAGTCC
ATCAAAACAGACTTCCTGCAGCCATGAAACCGTCTCAGGGGTCTGGTAGT
TTTGGAGCCATTCTGGGCAGCAGGACCTCACAGAAGGAAACCAGCAGGCA
GCTTTCTTACTCAGACAATCAGGCTTCTGCAAAAAGAGGAAGTTTGGAAA
CTAAAGATGATATTCCATTTCGAAAAGTTCTTGGTAATCCGGGTAGAGGA
TCGATTAAGACTGTAGCAGGAAGTGGAATAGCTCGGACGATTCCTTCTTT
GACATCTACTTCAACACCTCTTAGATCAGGGTTGCTAGAAAATCGTACTG
AAAAGAGGAAAAGAATGATATCAACTGGCTCAGAATTGAATGAAGATTAC
CCTAAGGAAAATGATTCATCATCGAACAACAAGGCCATGACAGATCCCTC
CAGAAAGTATTTAACCAGCAGTAGAGAAAAGCAGCTGAGTTTGAAACAGT
CAGAAGAGAATAGGACATCAGGGCTTTTACCTTTACAGTCATCATCCTTT
TATGGTAGCAGAGCTGGATCCAAGGAACACTCTTCTGGTGGCACTAACTT
AGACAGGACTAATGTTTCAAGCCAGACTCCCTCTGCCAAAAGAAGTTTGG
GATTTCTTCCTCAGCCAGTTCCTCTTTCTGTTAAAAAACTGAGGTGTAAC
CAGGATTACACTGGCTGGAATAAACCAAGAGTGCCCCTTTCCTCTCACCA
ACAGCAGCAACTGCAGGGCTTCTCCAATTTGGGAAATACCTGCTATATGA
ATGCTATTCTACAATCTCTATTTTCACTCCAGTCATTTGCAAATGACTTG
CTTAAACAAGGTATCCCATGGAAGAAAATTCCACTCAATGCACTTATCAG
ACGCTTTGCACACTTGCTTGTTAAAAAAGATATCTGTAATTCAGAGACCA
AAAAGGATTTACTCAAGAAGGTTAAAAATGCCATTTCAGCTACAGCAGAG
AGATTCTCTGGTTATATGCAGAATGATGCTCATGAATTTTTAAGTCAGTG
TTTGGACCAGCTGAAAGAAGATATGGAAAAATTAAATAAAACTTGGAAGA
CTGAACCTGTTTCTGGAGAAGAAAATTCACCAGATATTTCAGCTACCAGA
GCATACACTTGCCCTGTTATTACTAATTTGGAGTTTGAGGTTCAGCACTC
CATCATTTGTAAAGCATGTGGAGAGATTATCCCCAAAAGAGAACAGTTTA
ATGACCTCTCTATTGACCTTCCTCGTAGGAAAAAACCACTCCCTCCTCGT
TCAATTCAAGATTCTCTTGATCTTTTCTTTAGGGCCGAAGAACTGGAGTA
TTCTTGTGAGAAGTGTGGTGGGAAGTGTGCTCTTGTCAGGCACAAATTTA
ACAGGCTTCCTAGGGTCCTCATTCTCCATTTGAAACGATATAGCTTCAAT
GTGGCTCTCTCGCTTAACAATAAGATTGGGCAGCAAGTCATCATTCCAAG
ATACCTGACCCTGTCATCTCATTGCACTGAAAATACAAAACCACCTTTTA
CCCTTGGTTGGAGTGCACATATGGCAATTTCTAGACCATTGAAAGCCTCT
CAAATGGTGAATTCCTGCATCACCAGCCCTTCTACACCTTCAAAGAAATT
CACCTTCAAATCCAAGAGCTCCTTGGCTTTATGCCTTGATTCAGACAGTG
AGGATGAGCTAAAACGTTCTGTGGCCCTCAGCCAGAGACTTTGTGAAATG
TTAGGCAACGAACAGCAGCAGGAAGACCTGGAAAAAGATTCAAAATTATG
CCCAATAGAGCCTGACAAGTCTGAATTGGAAAACTCAGGATTTGACAGAA
TGAGCGAAGAAGAGCTTCTAGCAGCTGTCTTGGAGATAAGTAAGAGAGAT
GCTTCACCATCTCTGAGTCATGAAGATGATGATAAGCCAACTAGCAGCCC
AGATACCGGATTTGCAGAAGATGATATTCAAGAAATGCCAGAAAATCCAG
ACACTATGGAAACTGAGAAGCCCAAAACAATCACAGAGCTGGATCCTGCC
AGTTTTACTGAGATAACTAAAGACTGTGATGAGAATAAAGAAAACAAAAC
TCCAGAAGGATCTCAGGGAGAAGTTGATTGGCTCCAGCAGTATGATATGG
AGCGTGAAAGGGAAGAGCAAGAGCTTCAGCAGGCACTGGCTCAGAGCCTT
CAAGAGCAAGAGGCTTGGGAACAGAAAGAAGATGATGACCTCAAAAGAGC
TACCGAGTTAAGTCTTCAAGAGTTTAACAACTCCTTTGTGGATGCATTGG
GTTCTGATGAGGACTCTGGAAATGAGGATGTTTTTGATATGGAGTACACA
GAAGCTGAAGCTGAGGAACTGAAAAGAAATGCTGAGACAGGAAATCTGCC
TCATTCGTACCGGCTCATCAGTGTTGTCAGTCACATTGGTAGCACTTCTT
CTTCAGGTCATTACATTAGTGATGTATATGACATTAAGAAGCAAGCGTGG
TTTACTTACAATGACCTGGAGGTATCAAAAATCCAAGAGGCTGCCGTGCA
GAGTGATCGAGATCGGAGTGGCTACATCTTCTTTTATATGCACAAGGAGA
TCTTTGATGAGCTGCTGGAAACAGAAAAGAACTCTCAGTCACTTAGCACG
GAAGTGGGGAAGACTACCCGTCAGGCCTCGTGAGCGGCCGC

Amino Acid Sequence

MDYKDDDDKGIPGIPGSTRAAAMLTLQDNSFLSIDKVPSKDAEEMRLFLD
AVHQNRLPAAMKPSQGSGSFGAILGSRTSQKETSRQLSYSDNQASAKRGS
LETKDDIPFRKVLGNPGRGSIKTVAGSGIARTIPSLTSTSTPLRSGLLEN
RTEKRKRMISTGSELNEDYPKENDSSSNNKAMTDPSRKYLTSSREKQLSL
KQSEENRTSGLLPLQSSSFYGSRAGSKEHSSGGTNLDRTNVSSQTPSAKR
SLGFLPQPVPLSVKKLRCNQDYTGWNKPRVPLSSHQQQQLQGFSNLGNTC
YMNAILQSLFSLQSFANDLLKQGIPWKKIPLNALIRRFAHLLVKKDICNS
ETKKDLLKKVKNAISATAERFSGYMQNDAHEFLSQCLDQLKEDMEKLNKT
WKTEPVSGEENSPDISATRAYTCPVITNLEFEVQHSIICKACGEIIPKRE
QFNDLSIDLPRRKKPLPPRSIQDSLDLFFRAEELEYSCEKCGGKCALVRH
KFNRLPRVLILHLKRYSFNVALSLNNKIGQQVIIPRYLTLSSHCTENTKP
PFTLGWSAHMAISRPLKASQMVNSCITSPSTPSKKFTFKSKSSLALCLDS
DSEDELKRSVALSQRLCEMLGNEQQQEDLEKDSKLCPIEPDKSELENSGF
DRMSEEELLAAVLEISKRDASPSLSHEDDDKPTSSPDTGFAEDDIQEMPE
NPDTMETEKPKTITELDPASFTEITKDCDENKENKTPEGSQGEVDWLQQY
DMEREREEQELQQALAQSLQEQEAWEQKEDDDLKRATELSLQEFNNSFVD
ALGSDEDSGNEDVFDMEYTEAEAEELKRNAETGNLPHSYRLISVVSHIGS
TSSSGHYISDVYDIKKQAWFTYNDLEVSKIQEAAVQSDRDRSGYIFFYMH
KEIFDELLETEKNSQSLSTEVGKTTRQAS*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU15141
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee