ITCH

• Synonyms: ADMFD, AIF4, AIP4, NAPP1
• Plasmid: pCMV5-HA ITCH
• Parent Plasmid: pCMV5 HA1
• Expressed: HA-ITCH
• Genbank: NM_031483
• Tag: HA
• Species: Human

Sequence of Insert

GGATCCATGTCTGACAGTGGATCACAACTTGGTTCAATGGGTAGCCTCACCATGAAATCACAGCTTCAGATCACTGTCATCTCAGCAAAACTTAAGGAAAATAAGAAGAATTGGTTTGGACCAAGTCCTTACGTAGAGGTCACAGTAGATGGACAGTCAAAGAAGACAGAAAAATGCAACAACACAAACAGTCCCAAGTGGAAGCAACCCCTTACAGTTATCGTTACCCCTGTGAGTAAATTACATTTTCGTGTGTGGAGTCACCAGACACTGAAATCTGATGTTTTGTTGGGAACTGCTGCATTA GATATTTATGAAACATTAAAGTCAAACAATATGAAACTTGAAGAAGTAGT TGTGACTTTGCAGCTTGGAGGTGACAAAGAGCCAACAGAGACAATAGGAG ACTTGTCAATTTGTCTTGATGGGCTACAGTTAGAGTCTGAAGTTGTTACC AATGGTGAAACTACATGTTCAGAAAGTGCTTCTCAGAATGATGATGGCTC CAGATCCAAGGATGAAACAAGAGTGAGCACAAATGGATCAGATGACCCTG AAGATGCAGGAGCTGGTGAAAATAGGAGAGTCAGTGGGAATAATTCTCCA TCACTCTCAAATGGTGGTTTTAAACCTTCTAGACCTCCAAGACCTTCACG ACCACCACCACCCACCCCACGTAGACCAGCATCTGTCAATGGTTCACCAT CTGCCACTTCTGAAAGTGATGGGTCTAGTACAGGCTCTCTGCCGCCGACA AATACAAATACAAATACATCTGAAGGAGCAACATCTGGATTAATAATTCC TCTTACTATATCTGGAGGCTCAGGCCCTAGGCCATTAAATCCTGTAACTC AAGCTCCCTTGCCACCTGGTTGGGAGCAGAGAGTGGACCAGCACGGGCGA GTTTACTATGTAGATCATGTTGAGAAAAGAACAACATGGGATAGACCAGA ACCTCTACCTCCTGGCTGGGAACGGCGGGTTGACAACATGGGACGTATTT ATTATGTTGACCATTTCACAAGAACAACAACGTGGCAGAGGCCAACACTG GAATCCGTCCGGAACTATGAACAATGGCAGCTACAGCGTAGTCAGCTTCA AGGAGCAATGCAGCAGTTTAACCAGAGATTCATTTATGGGAATCAAGATT TATTTGCTACATCACAAAGTAAAGAATTTGATCCTCTTGGTCCATTGCCA CCTGGATGGGAGAAGAGAACAGACAGCAATGGCAGAGTATATTTCGTCAA CCACAACACACGAATTACACAATGGGAAGACCCCAGAAGTCAAGGTCAAT TAAATGAAAAGCCCTTACCTGAAGGTTGGGAAATGAGATTCACAGTGGAT GGAATTCCATATTTTGTGGACCACAATAGAAGAACTACCACCTATATAGA TCCCCGCACAGGAAAATCTGCCCTAGACAATGGACCTCAGATAGCCTATG TTCGGGACTTCAAAGCAAAGGTTCAGTATTTCCGGTTCTGGTGTCAGCAA CTGGCCATGCCACAGCACATAAAGATTACAGTGACAAGAAAAACATTGTT TGAGGATTCCTTTCAACAGATAATGAGCTTCAGTCCCCAAGATCTGCGAA GACGTTTGTGGGTGATTTTTCCAGGAGAAGAAGGTTTAGATTATGGAGGT GTAGCAAGAGAATGGTTCTTTCTTTTGTCACATGAAGTGTTGAACCCAAT GTATTGCCTGTTTGAATATGCAGGGAAGGATAACTACTGCTTGCAGATAA ACCCCGCTTCTTACATCAATCCAGATCACCTGAAATATTTTCGTTTTATT GGCAGATTTATTGCCATGGCTCTGTTCCATGGGAAATTCATAGACACGGG TTTTTCTTTACCATTCTATAAGCGTATCTTGAACAAACCAGTTGGACTCA AGGATTTAGAATCTATTGATCCAGAATTTTACAATTCTCTCATCTGGGTT AAGGAAAACAATATTGAGGAATGTGATTTGGAAATGTACTTCTCCGTTGA CAAAGAAATTCTAGGTGAAATTAAGAGTCATGATCTGAAACCTAATGGTG GCAATATTCTTGTAACAGAAGAAAATAAAGAGGAATACATCAGAATGGTA GCTGAGTGGAGGTTGTCTCGAGGTGTTGAAGAACAGACACAAGCTTTCTT TGAAGGCTTTAATGAAATTCTTCCCCAGCAATATTTGCAATACTTTGATG CAAAGGAATTAGAGGTCCTTTTATGTGGAATGCAAGAGATTGATTTGAAT GACTGGCAAAGACATGCCATCTACCGTCATTATGCAAGGACCAGCAAACA AATCATGTGGTTTTGGCAGTTTGTTAAAGAAATTGATAATGAGAAGAGAA TGAGACTTCTGCAGTTTGTTACTGGAACCTGCCGATTGCCAGTAGGAGGA TTTGCTGATCTCATGGGGAGCAATGGACCACAGAAATTCTGCATTGAAAA AGTTGGGAAAGAAAATTGGCTACCCAGAAGTCATACCTGTTTTAATCGCC TGGACCTGCCACCATACAAGAGCTATGAGCAACTGAAGGAAAAGCTGTTG TTTGCCATAGAAGAAACAGAAGGATTTGGACAAGAGTAAGCGGCCGC

Amino Acid Sequence

MYPYDVPDYAGSMSDSGSQLGSMGSLTMKSQLQITVISAKLKENKKNWFG
PSPYVEVTVDGQSKKTEKCNNTNSPKWKQPLTVIVTPVSKLHFRVWSHQT
LKSDVLLGTAALDIYETLKSNNMKLEEVVVTLQLGGDKEPTETIGDLSIC
LDGLQLESEVVTNGETTCSESASQNDDGSRSKDETRVSTNGSDDPEDAGA
GENRRVSGNNSPSLSNGGFKPSRPPRPSRPPPPTPRRPASVNGSPSATSE
SDGSSTGSLPPTNTNTNTSEGATSGLIIPLTISGGSGPRPLNPVTQAPLP
PGWEQRVDQHGRVYYVDHVEKRTTWDRPEPLPPGWERRVDNMGRIYYVDH
FTRTTTWQRPTLESVRNYEQWQLQRSQLQGAMQQFNQRFIYGNQDLFATS
QSKEFDPLGPLPPGWEKRTDSNGRVYFVNHNTRITQWEDPRSQGQLNEKP
LPEGWEMRFTVDGIPYFVDHNRRTTTYIDPRTGKSALDNGPQIAYVRDFK
AKVQYFRFWCQQLAMPQHIKITVTRKTLFEDSFQQIMSFSPQDLRRRLWV
IFPGEEGLDYGGVAREWFFLLSHEVLNPMYCLFEYAGKDNYCLQINPASY
INPDHLKYFRFIGRFIAMALFHGKFIDTGFSLPFYKRILNKPVGLKDLES
IDPEFYNSLIWVKENNIEECDLEMYFSVDKEILGEIKSHDLKPNGGNILV
TEENKEEYIRMVAEWRLSRGVEEQTQAFFEGFNEILPQQYLQYFDAKELE
VLLCGMQEIDLNDWQRHAIYRHYARTSKQIMWFWQFVKEIDNEKRMRLLQ
FVTGTCRLPVGGFADLMGSNGPQKFCIEKVGKENWLPRSHTCFNRLDLPP
YKSYEQLKEKLLFAIEETEGFGQE*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU19846
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee