50 mM Tris-HCl pH 7.5, 50 % glycerol, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.02 % Brij-35, 0.2 mM PMSF, 1 mM Benzamidine.
Gel Information :
Gel Image 1:
Amino acids S2 – A189 of human hnRNP A1.
[Full length protein ends at residue F372]
Residue G244 of the fusion protein is equivalent to S2 of the native protein. The GST tag is located at residues 1 – 220 and the HA tag is located at residues 235 – 243.
PreScission (LEVLFQGPL) residues 221 - 229
BamH1 of pGEX-6P-1
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