Recombinant Protein - FOXO1 / FKHR (2 - 655)

Name: FOXO1 / FKHR (2 - 655)
Price per aliquot (100µg): £110.00
Catalog Number (DU Number):
DU1725
Accession:
NM_002015
Expression:
bacteria
Tag and Terminus:
N-Term
GST
Uncleaved

C-Term
6His
Uncleaved
Purification Method:
GSH Sepharose and Ni2+-NTA agarose
Calculated molecular mass:
Mono-Isotopic Mass: 97, 115.33 daltons
Average Mass: 97, 177.39 daltons
Purity:
85 %
Storage Buffer:
50 mM Tris-HCl pH 7.5, 270 mM Sucrose, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.02 % Brij-35, 0.2 mM PMSF, 1 mM Benzamidine.
Storage Temperature:
–70 °C
Theoretical pI:
6.24
Gel Information :
  • Gel Image 1: 
Native Sequence:
Amino acids A2 – G655 (end) of human FKHR. Residues A232 of the fusion protein is equilivalent to A2 of the native protein. The GST tag is located at residues 1 – 220 and the His(6) tag is located at residues 886 – 891.
Protease Cleavage:
PreScission (LEVLFQGPL) residues 221 - 229
Cloning Sites:
BamHI and SalI sites of pGEX 6P-1

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