Human Recombinant Protein - PLK4 (1 - 269)

Name: PLK4 (1 - 269)
Price per aliquot (100µg): £110.00
Long Name:
Polo Like Kinase 4
Synonyms:
serine/threonine kinase 18, STK18, polo-like kinase 4, PLK-4
Species:
Human
Catalog Number (DU Number):
DU62994
Accession:
NM_014264.2
Expression:
bacteria
Tag and Terminus:
N-Term
GST
Uncleaved
Purification Method:
GSH-Agarose
Enzymatic Substrate:
Myelin Basic Protein Final concentration: 1 mg/ml
Calculated molecular mass:
Mono-Isotopic Mass: 57, 562.27 daltons
Average Mass: 57, 599.85 daltons
Protein Activity:
Constitutively Active
Purity:
>80 %
Storage Buffer:
50 mM Tris-HCl pH 7.5, 270 mM Sucrose, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.02 % Brij-35, 1 mM benzamidine, 0.2 mM PMSF
Storage Temperature:
-80 deg C
Theoretical PI:
6.71
Native Sequence:
Amino acids M1 – S269 (end residue is H970) of human PLK4. Residue M232 of the fusion protein is equivalent to M1 of the native enzyme. The GST tag is located at residues 1 – 220.
Protease Cleavage:
PreScission (LEVLFQGP) residues 221 – 228
Cloning Sites:
BamH1 sites of pGEX6P-1

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