Human Recombinant Protein - PLK1 (37 - 338)

Name: PLK1 (37 - 338)
Price per aliquot (100µg): £110.00
Long Name:
Polo Like Kinase 1
Synonyms:
PLK, polo-like kinase, polo-like kinase 1, PLK-1
Species:
Human
Catalog Number (DU Number):
DU61680
Accession:
NM_005030.6
Expression:
bacteria
Tag and Terminus:
N-Term
GST
Uncleaved
Purification Method:
GSH-Agarose
Enzymatic Substrate:
ISDELMDATFADQEAKKK
Calculated molecular mass:
Mono-Isotopic Mass: 61, 361.92 daltons
Average Mass: 61, 401.41 daltons
Protein Activity:
Constitutively Active
Purity:
>80 %
Storage Buffer:
50 mM Tris-HCl pH 7.5, 270 mM Sucrose, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.02 % Brij-35, 1 mM benzamidine, 0.2 mM PMSF
Storage Temperature:
-80 deg C
Theoretical PI:
8.54
Native Sequence:
Amino acids P37 – N338 (end residue is S603) of human PLK1. Residue P232 of the fusion protein is equivalent to P37 of the native enzyme. The GST tag is located at residues 1 – 220.
Protease Cleavage:
PreScission (LEVLFQGP) residues 221 – 228
Cloning Sites:
BamH1 sites of pGEX6P-1

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