[field_kinases_species] Recombinant Protein - AAK1 (1 - 365)

Name: AAK1 (1 - 365)
Price per aliquot (100µg): £110.00
Long Name:
AP2 associated kinase 1
Synonyms:
KIAA1048 , DKFZp686K16132
Catalog Number (DU Number):
DU61573
Accession:
NM_014911.5
Expression:
baculovirus
Tag and Terminus:
N-Term
GST
Uncleaved
Purification Method:
GSH-Agarose
Enzymatic Buffer:
50 mM Tris-HCl pH 7.5, 0.1mM EGTA, 10 mM DTT, 10 mM MgAc
Enzymatic Substrate:
Myelin Basic Protein Final concentration: 1 mg/ml
Calculated molecular mass:
Mono-Isotopic Mass: 67, 338.03 daltons
Average Mass: 67, 381.63 daltons
Protein Activity:
Constitutively Active
Purity:
>80 %
Storage Buffer:
50 mM Tris-HCl pH 7.5, 270 mM Sucrose, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.02 % Brij-35, 1 mM benzamidine, 0.2 mM PMSF
Storage Temperature:
-80 deg C
Theoretical PI:
6.39
Native Sequence:
Amino acids M1 – A365 (end residue is L961) of human AAK1. Residue M232 of the fusion protein is equivalent to M1 of the native enzyme. The GST tag is located at residues 1 – 220.
Protease Cleavage:
PreScission (LEVLFQGP) residues 221 – 228
Cloning Sites:
BamH1 and Not1 sites of pFastBac-GST

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