Name: NSP5 SARS-CoV2
Price per aliquot (100µg): £110.00
Long Name:
NSP5 (1 - 306) SARS-CoV2
Synonyms:
SARS-CoV2-NSP5, SARS-CoV-2 NSP5, NSP5 SARS-CoV-2, 3CLPro, 3CL-Pro
Catalog Number (DU Number):
DU67815
Accession:
QHD43415.1
Expression:
bacteria
Tag and Terminus:
N-Term
6His
Uncleaved
Purification Method:
Cobalt Agarose
Calculated molecular mass:
Mono-Isotopic Mass: 36, 217.60 daltons
Average Mass: 36, 241.27 daltons
Purity:
90 %
Storage Buffer:
50 mM Tris-HCl pH 7.5, 270 mM Sucrose, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.03 % Brij-35
Storage Temperature:
-70 °C
Theoretical PI:
6.20
Gel Information :
- Gel Image 1:
Native Sequence:
MGSSHHHHHHSSGLEVLFQGPLGSSGFRKMAFPSGKVEGCMVQVTCGTTTLNGLWLDDVVYCPRHVICTSEDMLNPNYEDLLIRKSNHNFLVQAGNVQLRVIGHSMQNCVLKLKVDTANPKTPKYKFVRIQPGQTFSVLACYNGSPSGVYQCAMRPNFTIKGSFLNGSCGSVGFNIDYDCVSFCYMHHMELPTGVHAGTDLEGNFYGPFVDRQTAQAAGTDTTITVNVLAWLYAAVINGDRWFLNRFTTTLNDFNLVAMKYNYEPLTQDHVDILGPLSAQTGIAVLDMCASLKELLQNGMNGRTILGSALLEDEFTPFDVVRQCSGVTFQ
Amino acids S1 – Q306 (end).
Residue S25 of the fusion protein is equivalent to S1 of the native enzyme.
The His6 tag is located at residues 5 – 10.
Protease Cleavage:
PreScission (LEVLFQGP) residues 14 - 21
