Recombinant Protein - N Protein Human CoV 229E

Name: N Protein Human CoV 229E
Price per aliquot (100µg): £110.00
Long Name:
Nucleocapsid Protein (1 - 389) Human CoV 229E
Synonyms:
Human CoV 229E N Protein, Human CoV 229E Nucleocapsid Protein
Catalog Number (DU Number):
DU 67739
Accession:
NP_073556.1
Expression:
bacteria
Tag and Terminus:
N-Term
MBP
Uncleaved
Purification Method:
Amylose Resin
Calculated molecular mass:
Mono-Isotopic Mass: 87, 651.64 daltons
Average Mass: 87, 705.68 daltons
Purity:
85 %
Storage Buffer:
50 mM Tris-HCl pH 7.5, 270 mM Sucrose, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.03 % Brij-35
Storage Temperature:
-70 °C
Theoretical PI:
7.67
Gel Information :
  • Gel Image 1: 
Native Sequence:
MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYEEELVKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQTNSSSNNNNNNNNNNLGDDDDKVPEFLEVLFQGPLGSMATVKWADASEPQRGRQGRIPYSLYSPLLVDSEQPWKVIPRNLVPINKKDKNKLIGYWNVQKRFRTRKGKRVDLSPKLHFYYLGTGPHKDAKFRERVEGVVWVAVDGAKTEPTGYGVRRKNSEPEIPHFNQKLPNGVTVVEEPDSRAPSRSQSRSQSRGRGESKPQSRNPSSDRNHNSQDDIMKAVAAALKSLGFDKPQEKDKKSAKTGTPKPSRNQSPASSQTSAKSLARSQSSETKEQKHEMQKPRWKRQPNDDVTSNVTQCFGPRDLDHNFGSAGVVANGVKAKGYPQFAELVPSTAAMLFDSHIVSKESGNTVVLTFTTRVTVPKDHPHLGKFLEELNAFTREMQQHPLLNPSALEFNPSQTSPATAEPVRDEVSIETDIIDEVN Amino acids M1 – N389 (end). Residue M404 of the fusion protein is equivalent to M1 of the native enzyme. The MBP tag is located at residues 1 – 367.
Protease Cleavage:
PreScission (LEVLFQGP) residues 393 - 400

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