Name: NSP15 SARS-CoV2
Price per aliquot (100µg): £110.00
Long Name:
NSP15 (1 - 527) SARS-CoV2
Synonyms:
SARS-CoV2-NSP15, SARS-CoV-2 NSP15, NSP15 SARS-CoV-2
Catalog Number (DU Number):
DU 67734
Accession:
QHD43415.1
Expression:
bacteria
Tag and Terminus:
N-Term
MBP
Uncleaved
Purification Method:
Amylose Resin
Calculated molecular mass:
Mono-Isotopic Mass: 83, 000.25 daltons
Average Mass: 83, 052.31 daltons
Purity:
80 %
Storage Buffer:
50 mM Tris-HCl pH 7.5, 270 mM Sucrose, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.03 % Brij-35
Storage Temperature:
-70 °C
Theoretical PI:
4.93
Gel Information :
- Gel Image 1:
Native Sequence:
MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYEEELVKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQTNSSSNNNNNNNNNNLGDDDDKVPEFLEVLFQGPLGSSLENVAFNVVNKGHFDGQQGEVPVSIINNTVYTKVDGVDVELFENKTTLPVNVAFELWAKRNIKPVPEVKILNNLGVDIAANTVIWDYKRDAPAHISTIGVCSMTDIAKKPTETICAPLTVFFDGRVDGQVDLFRNARNGVLITEGSVKGLQPSVGPKQASLNGVTLIGEAVKTQFNYYKKVDGVVQQLPETYFTQSRNLQEFKPRSQMEIDFLELAMDEFIERYKLEGYAFEHIVYGDFSHSQLGGLHLLIGLAKRFKESPFELEDFIPMDSTVKNYFITDAQTGSSKCVCSVIDLLLDDFVEIIKSQDLSVVSKVVKVTIDYTEISFMLWCKDGHVETFYPKLQ
Amino acids S1 – Q346 (end).
Residue S404 of the fusion protein is equivalent to S1 of the native enzyme.
The MBP tag is located at residues 1 – 367.
Protease Cleavage:
PreScission (LEVLFQGP) residues 393 – 400
