Human Recombinant Protein - PKB gamma (117 - 479)

Name: PKB gamma (117 - 479)
Price per aliquot (100µg): £110.00
Long Name:
AKT serine/threonine kinase 3
Synonyms:
AKT3, PKBG, RAC-gamma, PRKBG, v-akt murine thymoma viral oncogene homolog 3, protein kinase B gamma, PKBgamma
Species:
Human
Catalog Number (DU Number):
DU62934
Accession:
NM_005465.7
Expression:
baculovirus
Tag and Terminus:
N-Term
6His
Uncleaved
Purification Method:
Cobalt Agarose
Enzymatic Buffer:
50 mM Tris-HCl pH 7.5, 0.1 mM EGTA, 10 mM DTT, 10 mM Magnesium acetate
Enzymatic Substrate:
GRPRTSSFAEGKK
Calculated molecular mass:
Mono-Isotopic Mass: 45, 355.23 daltons
Average Mass: 45, 384.48 daltons
Protein Activity:
Constitutively Active
Purity:
>80 %
Storage Buffer:
50 mM Tris-HCl pH 7.5, 150 mM NaCl, 270 mM sucrose, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.02 % Brij-35, 1 mM benzamidine, 0.2 mM PMSF
Storage Temperature:
-80 deg C
Theoretical PI:
5.76
Native Sequence:
Amino acids M117 – E479 (end) of human PKB gamma. Residue M30 of the fusion protein is equivalent to M117 of the native enzyme. The His6 tag is located at residues 5 – 10.
Protease Cleavage:
rTEV (ENLYFQG) residues 18 – 24
Cloning Sites:
BglII EcoR1 into BamH1 EcoR1 sites into pFastBac HTb

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