[field_kinases_species] Recombinant Protein - CHK1 (1 - 476)
Name: CHK1 (1 - 476)
Price per aliquot (100µg): £110.00
Catalog Number (DU Number):
Enzymatic Assay Format:
Standard filter binding assay
50 mM Tris-HCl pH 7.5, 0.1 % 2 mercaptoethanol, 0.1 mM EGTA, 0.02 % Brij-35, 10 mM magnesium acetate
CHKtide [KKKVSRSGLYRSPSMPENLNRPR]; Final concentration: 30 μM
Calculated molecular mass:
Mono-Isotopic Mass: 81,564.69
50 mM Tris-HCl pH 7.5, 270 mM Sucrose, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.02 % Brij-35
Amino acids M1 – T476 (end) of human CHK1. ResidueM235 of the fusion protein is equivalent to M1 of the native enzyme. The GST tag is located between residues 1 - 220 of the fusion protein.
PreScission site (LEVLFQGPL) residues 221-229
EcoR1 and Not1 of pFastBac GST
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