cDNA Clone - SARS-CoV2 (2019-nCoV) NSP11/12

Name: SARS-CoV2 (2019-nCoV) NSP11/12
Price per aliquot: £125.00
Expressed:
MBP 3C SARS-CoV-2 NSP11/12
Plasmid:
pMEX3Cb SARS-CoV-2 NSP11/12
Full Snapgene Map:
Binary Data pMEX3Cb 2019 nCoV Nsp11 12 67851.dna
Parental Plasmid:
pMEX3Cb
View Parent Plasmid

Snapgene File | Genbank File
acgttatcgactgcacggtgcaccaatgcttctggcgtcaggcagccatcggaagctgtggtatggctgtgcaggtcgtaaatcactgcataattcgtgtcgctcaaggcgcactcccgttctggataatgttttttgcgccgacatcataacggttctggcaaatattctgaaatgagctgttgacaATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGAAACAGTATTCATATGATGAAAATCGAAGAAGGTAAACTGGTAATCTGGATTAACGGCGATAAAGGCTATAACGGTCTCGCTGAAGTCGGTAAGAAATTCGAGAAAGATACCGGAATTAAAGTCACCGTTGAGCATCCGGATAAACTGGAAGAGAAATTCCCACAGGTTGCGGCAACTGGCGATGGCCCTGACATTATCTTCTGGGCACACGACCGCTTTGGTGGCTACGCTCAATCTGGCCTGTTGGCTGAAATCACCCCGGACAAAGCGTTCCAGGACAAGCTGTATCCGTTTACCTGGGATGCCGTACGTTACAACGGCAAGCTGATTGCTTACCCGATCGCTGTTGAAGCGTTATCGCTGATTTATAACAAAGATCTGCTGCCGAACCCGCCAAAAACCTGGGAAGAGATCCCGGCGCTGGATAAAGAACTGAAAGCGAAAGGTAAGAGCGCGCTGATGTTCAACCTGCAAGAACCGTACTTCACCTGGCCGCTGATTGCTGCTGACGGGGGTTATGCGTTCAAGTATGAAAACGGCAAGTACGACATTAAAGACGTGGGCGTGGATAACGCTGGCGCGAAAGCGGGTCTGACCTTCCTGGTTGACCTGATTAAAAACAAACACATGAATGCAGACACCGATTACTCCATCGCAGAAGCTGCCTTTAATAAAGGCGAAACAGCGATGACCATCAACGGCCCGTGGGCATGGTCCAACATCGACACCAGCAAAGTGAATTATGGTGTAACGGTACTGCCGACCTTCAAGGGTCAACCATCCAAACCGTTCGTTGGCGTGCTGAGCGCAGGTATTAACGCCGCCAGTCCGAACAAAGAACTGGCAAAAGAGTTCCTCGAAAACTATCTGCTGACTGATGAAGGTCTGGAAGCGGTTAATAAAGACAAACCGCTGGGTGCCGTAGCGCTGAAGTCTTACGAGGAAGAGTTGGTGAAAGATCCGCGGATTGCCGCCACTATGGAAAACGCCCAGAAAGGTGAAATCATGCCGAACATCCCGCAGATGTCCGCTTTCTGGTATGCCGTGCGTACTGCGGTGATCAACGCCGCCAGCGGTCGTCAGACTGTCGATGAAGCCCTGAAAGACGCGCAGACTAATTCGAGCTCGAACAACAACAACAATAACAATAACAACAACCTCGGGGATGACGATGACAAGGTACCGGAGTTCCTGGAAGTTCTGTTCCAGGGGCCCCTGGGATCCCCCGGGGAATTCGCTAGCCTCGAGAAGCTTGCGGCCGCTCAATTGgccgcatcgtgactgactgacgatctgcctcgcgcgtttcggtgatgacggtgaaaacctctgacacatgcagctcccggagacggtcacagcttgtctgtaagcggatgccgggagcagacaagcccgtcagggcgcgtcagcgggtgttggcgggtgtcggggcgcagccatgacccagtcacgtagcgatagcggagtgtataattcttgaagacgaaagggcctcgtgatacgcctatttttataggttaatgtcatgataataatggtttcttagacgtcaggtggcacttttcggggaaatgtgcgcggaacccctatttgtttatttttctaaatacattcaaatatgtatccgctcatgagacaataaccctgataaatgcttcaataatattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttacatcgaactggatctcaacagcggtaagatccttgagagttttcgccccgaagaacgttttccaatgatgagcacttttaaagttctgctatgtggcgcggtattatcccgtgttgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagtcacagaaaagcatcttacggatggcatgacagtaagagaattatgcagtgctgccataaccatgagtgataacactgcggccaacttacttctgacaacgatcggaggaccgaaggagctaaccgcttttttgcacaacatgggggatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgcagcaatggcaacaacgttgcgcaaactattaactggcgaactacttactctagcttcccggcaacaattaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtgggtctcgcggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcactgattaagcattggtaactgtcagaccaagtttactcatatatactttagattgatttaaaacttcatttttaatttaaaaggatctaggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggcttcagcagagcgcagataccaaatactgtccttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcacatgttctttcctgcgttatcccctgattctgtggataaccgtattaccgcctttgagtgagctgataccgctcgccgcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcctgatgcggtattttctccttacgcatctgtgcggtatttcacaccgcataaattccgacaccatcgaatggtgcaaaacctttcgcggtatggcatgatagcgcccggaagagagtcaattcagggtggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgtcaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacaggaaacagctatgaccatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgctttgcctggtttccggcaccagaagcggtgccggaaagctggctggagtgcgatcttcctgaggccgatactgtcgtcgtcccctcaaactggcagatgcacggttacgatgcgcccatctacaccaacgtaacctatcccattacggtcaatccgccgtttgttcccacggagaatccgacgggttgttactcgctcacatttaatgttgatgaaagctggctacaggaaggccagacgcgaattatttttgatggcgttggaatt
DU Number:
DU67851
Genbank:
MN908947.3
Species:
SARS-CoV-2
Sequence of Insert:
View Sequence

GGATCCAACCGCGTGTGCGGCGTGAGCGCGGCGCGCCTGACCCCGTGCGGCACCGGCACCAGCACCGATGTGGTGTATCGCGCGTTTGATATTTATAACGATAAAGTGGCGGGCTTTGCGAAATTTCTGAAAACCAACTGCTGCCGCTTTCAGGAAAAAGATGAAGATGATAACCTGATTGATAGCTATTTTGTGGTGAAACGCCATACCTTTAGCAACTATCAGCATGAAGAAACCATTTATAACCTGCTGAAAGATTGCCCGGCGGTGGCGAAACATGATTTTTTTAAATTTCGCATTGATGGCGATATGGTGCCGCATATTAGCCGCCAGCGCCTGACCAAATATACCATGGCGGATCTGGTGTATGCGCTGCGCCATTTTGATGAAGGCAACTGCGATACCCTGAAAGAAATTCTGGTGACCTATAACTGCTGCGATGATGATTATTTTAACAAAAAAGATTGGTATGATTTTGTGGAAAACCCGGATATTCTGCGCGTGTATGCGAACCTGGGCGAACGCGTGCGCCAGGCGCTGCTGAAAACCGTGCAGTTTTGCGATGCGATGCGCAACGCGGGCATTGTGGGCGTGCTGACCCTGGATAACCAGGATCTGAACGGCAACTGGTATGATTTTGGCGATTTTATTCAGACCACCCCGGGCAGCGGCGTGCCGGTGGTGGATAGCTATTATAGCCTGCTGATGCCGATTCTGACCCTGACCCGCGCGCTGACCGCGGAAAGCCATGTGGATACCGATCTGACCAAACCGTATATTAAATGGGATCTGCTGAAATATGATTTTACCGAAGAACGCCTGAAACTGTTTGATCGCTATTTTAAATATTGGGATCAGACCTATCATCCGAACTGCGTGAACTGCCTGGATGATCGCTGCATTCTGCATTGCGCGAACTTTAACGTGCTGTTTAGCACCGTGTTTCCGCCGACCAGCTTTGGCCCGCTGGTGCGCAAAATTTTTGTGGATGGCGTGCCGTTTGTGGTGAGCACCGGCTATCATTTTCGCGAACTGGGCGTGGTGCATAACCAGGATGTGAACCTGCATAGCAGCCGCCTGAGCTTTAAAGAACTGCTGGTGTATGCGGCGGAcCCGGCGATGCATGCGGCGAGCGGCAACCTGCTGCTGGATAAACGCACCACCTGCTTTAGCGTGGCGGCGCTGACCAACAACGTGGCGTTTCAGACCGTGAAACCGGGCAACTTTAACAAAGATTTTTATGATTTTGCGGTGAGCAAAGGCTTTTTTAAAGAAGGCAGCAGCGTGGAACTGAAACATTTTTTTTTTGCGCAGGATGGCAACGCGGCGATTAGCGATTATGATTATTATCGCTATAACCTGCCGACCATGTGCGATATTCGCCAGCTGCTGTTTGTGGTGGAAGTGGTGGATAAATATTTTGATTGCTATGATGGCGGCTGCATTAACGCGAACCAGGTGATTGTGAACAACCTGGATAAAAGCGCGGGCTTTCCGTTTAACAAATGGGGCAAAGCGCGCCTGTATTATGATAGCATGAGCTATGAAGATCAGGATGCGCTGTTTGCGTATACCAAACGCAACGTGATTCCGACCATTACCCAGATGAACCTGAAATATGCGATTAGCGCGAAAAACCGCGCGCGCACCGTGGCGGGCGTGAGCATTTGCAGCACCATGACCAACCGCCAGTTTCATCAGAAACTGCTGAAAAGCATTGCGGCGACCCGCGGCGCGACCGTGGTGATTGGCACCAGCAAATTTTATGGCGGCTGGCATAACATGCTGAAAACCGTGTATAGCGATGTGGAAAACCCGCATCTGATGGGCTGGGATTATCCGAAATGCGATCGCGCGATGCCGAACATGCTGCGCATTATGGCGAGCCTGGTGCTGGCGCGCAAACATACCACCTGCTGCAGCCTGAGCCATCGCTTTTATCGCCTGGCGAACGAATGCGCGCAGGTGCTGAGCGAAATGGTGATGTGCGGCGGCAGCCTGTATGTGAAACCGGGCGGCACCAGCAGCGGCGATGCGACCACCGCGTATGCGAACAGCGTGTTTAACATTTGCCAGGCGGTGACCGCGAACGTGAACGCGCTGCTGAGCACCGATGGCAACAAAATTGCGGATAAATATGTGCGCAACCTGCAGCATCGCCTGTATGAATGCCTGTATCGCAACCGCGATGTGGATACCGATTTTGTGAACGAATTTTATGCGTATCTGCGCAAACATTTTAGCATGATGATTCTGAGCGATGATGCGGTGGTGTGCTTTAACAGCACCTATGCGAGCCAGGGCCTGGTGGCGAGCATTAAAAACTTTAAAAGCGTGCTGTATTATCAGAACAACGTGTTTATGAGCGAAGCGAAATGCTGGACCGAAACCGATCTGACCAAAGGCCCGCATGAATTTTGCAGCCAGCATACCATGCTGGTGAAACAGGGCGATGATTATGTGTATCTGCCGTATCCGGAcCCGAGCCGCATTCTGGGCGCGGGCTGCTTTGTGGATGATATTGTGAAAACCGATGGCACCCTGATGATTGAACGCTTTGTGAGCCTGGCGATTGATGCGTATCCGCTGACCAAACATCCGAACCAGGAATATGCGGATGTGTTTCATCTGTATCTGCAGTATATTCGCAAACTGCATGATGAACTGACCGGCCATATGCTGGATATGTATAGCGTGATGCTGACCAACGATAACACCAGCCGCTATTGGGAACCGGAATTTTATGAAGCGATGTATACCCCGCATACCGTGCTGCAGtgaGCGGCCGC
Amino Acid Sequence:
View Sequence

MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRY NGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLI KNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPL GAVALKSYEEELVKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQTNSSSNNNNNNNNNNLGDDDDKVPEFLEVLFQGP LGSNRVCGVSAARLTPCGTGTSTDVVYRAFDIYNDKVAGFAKFLKTNCCRFQEKDEDDNLIDSYFVVKRHTFSNYQHEETIYNLLKDCPAVAKHDFFKFR IDGDMVPHISRQRLTKYTMADLVYALRHFDEGNCDTLKEILVTYNCCDDDYFNKKDWYDFVENPDILRVYANLGERVRQALLKTVQFCDAMRNAGIVGVL TLDNQDLNGNWYDFGDFIQTTPGSGVPVVDSYYSLLMPILTLTRALTAESHVDTDLTKPYIKWDLLKYDFTEERLKLFDRYFKYWDQTYHPNCVNCLDDR CILHCANFNVLFSTVFPPTSFGPLVRKIFVDGVPFVVSTGYHFRELGVVHNQDVNLHSSRLSFKELLVYAADPAMHAASGNLLLDKRTTCFSVAALTNNV AFQTVKPGNFNKDFYDFAVSKGFFKEGSSVELKHFFFAQDGNAAISDYDYYRYNLPTMCDIRQLLFVVEVVDKYFDCYDGGCINANQVIVNNLDKSAGFP FNKWGKARLYYDSMSYEDQDALFAYTKRNVIPTITQMNLKYAISAKNRARTVAGVSICSTMTNRQFHQKLLKSIAATRGATVVIGTSKFYGGWHNMLKTV YSDVENPHLMGWDYPKCDRAMPNMLRIMASLVLARKHTTCCSLSHRFYRLANECAQVLSEMVMCGGSLYVKPGGTSSGDATTAYANSVFNICQAVTANVN ALLSTDGNKIADKYVRNLQHRLYECLYRNRDVDTDFVNEFYAYLRKHFSMMILSDDAVVCFNSTYASQGLVASIKNFKSVLYYQNNVFMSEAKCWTETDL TKGPHEFCSQHTMLVKQGDDYVYLPYPDPSRILGAGCFVDDIVKTDGTLMIERFVSLAIDAYPLTKHPNQEYADVFHLYLQYIRKLHDELTGHMLDMYSV MLTNDNTSRYWEPEFYEAMYTPHTVLQ*
Bacterial Selection:
Amp
Low Growth Temperature Requirement?:
No
Comments:
Codon optimised for bacterial expression.

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