cDNA Clone - RAB8B

Name: RAB8B
Price per aliquot: £110.00
Plasmid:
pMK-RQ RAB8B Nter HA donor
Parent Plasmid:
pMK-RQ
View Parent Plasmid

Snapgene File | Genbank File
ctaaattgtaagcgttaatattttgttaaaattcgcgttaaatttttgttaaatcagctcattttttaaccaataggccgaaatcggcaaaatcccttataaatcaaaagaatagaccgagatagggttgagtggccgctacagggcgctcccattcgccattcaggctgcgcaactgttgggaagggcgtttcggtgcgggcctcttcgctattacgccagctggcgaaagggggatgtgctgcaaggcgattaagttgggtaacgccagggttttcccagtcacgacgttgtaaaacgacggccagtgagcgcgacgtaatacgactcactatagggcgaattgaaggaaggccgtcaaggccgcatccctgggcctcatgggccttcctttcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaacatggtcatagctgtttccttgcgtattgggcgctctccgcttcctcgctcactgactcgctgcgctcggtcgttcgggtaaagcctggggtgcctaatgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaagaacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttattagaaaaattcatccagcagacgataaaacgcaatacgctggctatccggtgccgcaatgccatacagcaccagaaaacgatccgcccattcgccgcccagttcttccgcaatatcacgggtggccagcgcaatatcctgataacgatccgccacgcccagacggccgcaatcaataaagccgctaaaacggccattttccaccataatgttcggcaggcacgcatcaccatgggtcaccaccagatcttcgccatccggcatgctcgctttcagacgcgcaaacagctctgccggtgccaggccctgatgttcttcatccagatcatcctgatccaccaggcccgcttccatacgggtacgcgcacgttcaatacgatgtttcgcctgatgatcaaacggacaggtcgccgggtccagggtatgcagacgacgcatggcatccgccataatgctcactttttctgccggcgccagatggctagacagcagatcctgacccggcacttcgcccagcagcagccaatcacggcccgcttcggtcaccacatccagcaccgccgcacacggaacaccggtggtggccagccagctcagacgcgccgcttcatcctgcagctcgttcagcgcaccgctcagatcggttttcacaaacagcaccggacgaccctgcgcgctcagacgaaacaccgccgcatcagagcagccaatggtctgctgcgcccaatcatagccaaacagacgttccacccacgctgccgggctacccgcatgcaggccatcctgttcaatcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccac
DU Number:
DU48994
Genbank:
Species:
Human
Sequence of Insert:
View Sequence

gcggccgcAAGGAAAGAAAGCATCTCTGCTCCAGGTCTCGGCGGAAGAGATGCGGGAACTACGGGGCTCTGATTCTGAGGTCCTGGATTTGGAAGGCGTCTGCACACAAAGAAATAACAAAGTTAAGTTCCCGCGGCTCGACCAGCAGAGTACGAAACGGGGCCTCCCTTCCCAGCTCGGGTGGAAACGCCTCCACCCTGTCACCCCGCCCATAGCAGGTCTTTTATCCGCTGGAGCCGCCTGGACAGTCCTCGCGGGGCACGCCCGAGGCTCCCCGGAGGAACTACAAGTCCCATGAAGTCCCGGGGCGCGGGCGGTGATGACGAAGCCCTCGCCGATTGGCCGCACGGGGCGGGGCGCGCAGGCCTTCTCCGAGAGGAGGGGCGGGGCGTGGGGCGGGGCGAGCAGCGGCGGATTGGCGGGCACGCCCCCTCGCCCGCGGCCCCCTCCCCGCCTCTCTCCACCGCCTCCTCTGGCTCCCCGGTCAGAGGGCCGGAGCGAGAAGATGTACCCATACGATGTGCCAGATTACGCCGCGAAGACGTACGATTATCTCTTCAAGCTCCTGttaATCGGtGACagcGGGGTAGGCAAGACCTGCCTCCTGTTCCGCTTCTCAGAGGACGCCTTCAACACCACCTTCATCTCCACCATCGGTGAGGGAGGGGCCGCGGCCCGGGACCGGGTAGAGAATTGGGGGGCGGGTACTAGGGGACGGGGAAAGGGCGGGGGGCGCTTGGGAGCCCGGGAGAGGAGACCCCGGGGACTGCAAGGTGGCGGGGGCACTGAGAGGGGCGAGGGCGTGAGGGAGAGGGGCGAGGGCGTGAGGGAGAGAGGCGAGCGGGAACGTGAGGCTAAGGGCGAAGGGGGAACTTGGGGGCAAAGGAACGGGAAGGGGAACAGAGGAACAAGGACTAGGGGTATTGGGGGAACGAGAGGGTTCGGAGACTGACAGGGAGGGGTCTTTGAGGGACAAAATGTGTTGGGGGGAAGACTCAGGAAATCTCATGTAGAGAGAAGGGGATAGTGGACGTGCCAATGATTAAAAGACAGGAGAGGAAGGAAATGTGAAGGGGGAAATGAGcggccgc
Amino Acid Sequence:
View Sequence

Antibiotic:
Kan
Comments:
CRISPR donor plasmid. Designed to KI an HA tag to the N-terminus of RAB8B when co-transfected with guides 48935 and 48949. Donor sequence shown as sequence of insert. Positives may be enriched for with puromycin prior to further selection by western blotting and FACs. Note this system does not require induction with Tet/Dox. May be used in any cell-line, contains several silent mutations to prevent binding of guides.

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