cDNA Clone - RAB1A

Name: RAB1A
Price per aliquot: £110.00
Expressed:
RAB1A Nter KI antisense guide + Cas9 D10A
Plasmid:
pX335 RAB1A Nter KI antisense
Parent Plasmid:
pX335
View Parent Plasmid

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DU Number:
DU48951
Genbank:
Species:
Human
Synonyms:
RAB1, YPT1
Sequence of Insert:
View Sequence

gTCACTATTCGGGATTCATGC
Amino Acid Sequence:
View Sequence

Antibiotic:
Amp
Comments:
CRISPR guide plasmid. Targets antisense strand of exon 1, designed to KI an HA tag to the N-terminus of RAB1A when co-transfected with sense guide 48937 and donor 48990. Guide sequence shown as sequence of insert. Positives may be enriched for with puromycin prior to further selection by western blotting and FACs. Note this system does not require induction with Tet/Dox. May be used in any cell-line though an empty TRex line is preferable as this allows for downstream rescue and mutant analysis if necessary.

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