cDNA Clone - RAB8A

Name: RAB8A
Price per aliquot: £110.00
Expressed:
RAB8A KO antisense guide + Cas9 D10A
Plasmid:
pX335 RAB8A KO as
Parent Plasmid:
pX335
View Parent Plasmid

|
DU Number:
DU48942
Genbank:
Species:
Human
Synonyms:
MEL, RAB8
Sequence of Insert:
View Sequence

gATATTACACTCTCTCCCCGA
Amino Acid Sequence:
View Sequence

Antibiotic:
Amp
Comments:
CRISPR guide plasmid. Targets antisense strand of exon 1, designed to KO RAB8A when co-transfected with sense guide 48928. Guide sequence shown as sequence of insert. Positives may be enriched for with puromycin prior to further selection by western blotting and FACs. Note this system does not require induction with Tet/Dox. May be used in any cell-line though an empty TRex line is preferable as this allows for downstream rescue and mutant analysis if necessary.

Do you need any help? Please get in touch and we’ll be happy to lend a hand.