This antibody detects SIK3, but does not detect SIK1 nor does it detect SIK2. This antibody works well with overexpressed protein. HEK293 cells were transfected with vectors encoding HA-SIK1, HA-SIK2 or HA-SIK3. Cell extracts were then blotted using the anti-SIK3 (62 - 313) S372D.