Doublet recognised at the expected size (80-90 kDa). The upper of the two bands corresponds to the NEDD8-conjugated form of the cullin. Please note - Additional non-specific bands may be seen depending on lysis procedure and cell type. Can detect Cullin5 in HEK293, U2OS and DT40 (chicken) cell lines.
30 ug of antibody non-covalently coupled to Protein G-Sepharose successfully immunoprecipitated endogenous Cullin5 from 3 mg of HEK293 cell lysate.