Polyclonal Antibody - VPS34

Name: VPS34
Price per aliquot (100µg): £110.00
Full Name:
VPS34 phospho Ser 282
Long Name Text:
phosphoinositide-3-kinase, class 3
Immuno Sequence:
RSLRSGPS*DHDL [residues 275 - 286 of human]
Phosphopeptide:
RSLRSGPSDHDL [residues 275 - 286 of human]
Sheep Number:
S375C
Monoclonal/Polyclonal:
Polyclonal
Purification Method:
Affinity purified against phospho specific peptide.
Gel Image:

HEK 293 cells were stimulated (+) / not stimulated (-) with various AMPK activators. VPS34 was immunoprecipitated from 1mg HEK 293 cellular extracts using 1 ug of anti- VPS34[S672B]. ImmunoprecipitatesweresubjectedtoSDS-PAGEanalysisbeforebeing transferred to nitrocellulose membrane and immunoblotted with anti-VPS34 [S672B] or anti-VPS34 phospho Ser 282 [S375C]. Binding of the primary antibody was detected using rabbit peroxidase conjugated anti- sheep IgG antibody (1 in 2500 dilution) and enhanced chemiluminescence.
Recombinant VPS34 wildtype or S282A was phosphorylated by AMPK in vitro and SDS-PAGE analysis before being transferred to nitrocellulose membrane and immunoblotted with various bleeds of anti-VPS34 phospho Ser 282 [S375C]. Binding of the primary antibody was detected using rabbit peroxidase conjugated anti- sheep IgG antibody (1 in 2500 dilution) and enhanced chemiluminescence.

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