cDNA Clone DDB1

• Plasmid: pCMV-HA-2 DDB1
• Parent Plasmid: pCMV5 HA2
• Expressed: HA-DDB1
• Genbank: NP_001914.2
• Tag: HA

Sequence of Insert

GCGGCCGCGATGTCGTACAACTACGTGGTAACGGCCCAGAAGCCCACCGC
CGTGAACGGCTGCGTGACCGGACACTTTACTTCGGCCGAAGACTTAAACC
TGTTGATTGCCAAAAACACGAGATTAGAGATCTATGTGGTCACCGCCGAG
GGGCTTCGGCCCGTCAAAGAGGTGGGCATGTATGGGAAGATTGCGGTCAT
GGAGCTTTTCAGGCCCAAGGGGGAGAGCAAGGACCTGCTGTTTATCTTGA
CAGCGAAGTACAATGCCTGCATCCTGGAGTATAAACAGAGTGGCGAGAGC
ATTGACATCATTACGCGAGCCCATGGCAATGTCCAGGACCGCATTGGCCG
CCCCTCAGAGACCGGCATTATTGGCATCATTGACCCTGAGTGCCGGATGA
TTGGCCTGCGTCTCTATGATGGCCTTTTCAAGGTTATTCCACTAGATCGC
GATAATAAAGAACTCAAGGCCTTCAACATCCGCCTGGAGGAGCTGCATGT
CATTGATGTCAAGTTCCTATATGGTTGCCAAGCACCTACTATTTGCTTTG
TCTACCAGGACCCTCAGGGGCGGCACGTAAAAACCTATGAGGTGTCTCTC
CGAGAAAAGGAATTCAATAAGGGCCCTTGGAAACAGGAAAATGTCGAAGC
TGAAGCTTCCATGGTGATCGCAGTCCCAGAGCCCTTTGGGGGGGCCATCA
TCATTGGACAGGAGTCAATCACCTATCACAATGGTGACAAATACCTGGCT
ATTGCCCCTCCTATCATCAAGCAAAGCACGATTGTGTGCCACAATCGAGT
GGACCCTAATGGCTCAAGATACCTGCTGGGAGACATGGAAGGCCGGCTCT
TCATGCTGCTTTTGGAGAAGGAGGAACAGATGGATGGCACCGTCACTCTC
AAGGATCTCCGTGTAGAACTCCTTGGAGAGACCTCTATTGCTGAGTGCTT
GACATACCTTGATAATGGTGTTGTGTTTGTCGGGTCTCGCCTGGGTGACT
CCCAGCTTGTGAAGCTCAACGTTGACAGTAATGAACAAGGCTCCTATGTA
GTGGCCATGGAAACCTTTACCAACTTAGGACCCATTGTCGATATGTGCGT
GGTGGACCTGGAGAGGCAGGGGCAGGGGCAGCTGGTCACTTGCTCTGGGG
CTTTCAAGGAAGGTTCTTTGCGGATCATCCGGAATGGAATTGGAATCCAC
GAGCATGCCAGCATTGACTTACCAGGCATCAAAGGATTATGGCCACTGCG
GTCTGACCCTAATCGTGAGACTTATGACACTTTGGTGCTCTCTTTTGTGG
GCCAGACAAGAGTTCTCATGTTAAATGGAGAGGAGGTAGAAGAAACCGAA
CTGATGGGTTTCGTGGATGATCAGCAGACTTTCTTCTGTGGCAACGTGGC
TCATCAGCAGCTTATCCAGATCACTTCAGCATCGGTGAGGTTGGTCTCTC
AAGAACCCAAAGCTCTGGTCAGTGAATGGAAGGAGCCTCAGGCCAAGAAC
ATCAGTGTGGCCTCCTGCAATAGCAGCCAGGTGGTGGTGGCTGTAGGCAG
GGCCCTCTACTATCTGCAGATCCATCCTCAGGAGCTCCGGCAGATCAGCC
ACACAGAGATGGAACATGAAGTGGCTTGCTTGGACATCACCCCATTAGGA
GACAGCAATGGACTGTCCCCTCTTTGTGCCATTGGCCTCTGGACGGACAT
CTCGGCTCGTATCTTGAAGTTGCCCTCTTTTGAACTACTGCACAAGGAGA
TGCTGGGTGGAGAGATCATTCCTCGCTCCATCCTGATGACCACCTTTGAG
AGTAGCCATTACCTCCTTTGTGCCTTGGGAGATGGAGCGCTTTTCTACTT
TGGGCTCAACATTGAGACAGGTCTGTTGAGCGACCGTAAGAAGGTGACTT
TGGGCACCCAGCCCACCGTATTGAGGACTTTTCGTTCTCTTTCTACCACC
AACGTCTTTGCTTGTTCTGACCGCCCCACTGTCATCTATAGCAGCAACCA
CAAATTGGTCTTCTCAAATGTCAACCTCAAGGAAGTGAACTACATGTGTC
CCCTCAATTCAGATGGCTATCCTGACAGCCTGGCGCTGGCCAACAATAGC
ACCCTCACCATTGGCACCATCGATGAGATCCAGAAGCTGCACATTCGCAC
AGTTCCCCTCTATGAGTCTCCAAGGAAGATCTGCTACCAGGAAGTGTCCC
AGTGTTTCGGGGTCCTCTCCAGCCGCATTGAAGTCCAAGACACGAGTGGG
GGCACGACAGCCTTGAGGCCCAGCGCTAGCACCCAGGCTCTGTCCAGCAG
TGTAAGCTCCAGCAAGCTGTTCTCCAGCAGCACTGCTCCTCATGAGACCT
CCTTTGGAGAAGAGGTGGAGGTGCATAACCTACTTATCATTGACCAACAC
ACCTTTGAAGTGCTTCATGCCCACCAGTTTCTGCAGAATGAATATGCCCT
CAGTCTGGTTTCCTGCAAGCTGGGCAAAGACCCCAACACTTACTTCATTG
TGGGCACAGCAATGGTGTATCCTGAAGAGGCAGAGCCCAAGCAGGGTCGC
ATTGTGGTCTTTCAGTATTCGGATGGAAAACTACAGACTGTGGCTGAAAA
GGAAGTGAAAGGGGCCGTGTACTCTATGGTGGAATTTAACGGGAAGCTGT
TAGCCAGCATCAATAGCACGGTGCGGCTCTATGAGTGGACAACAGAGAAG
GACGTGCGCACTGAGTGCAACCACTACAACAACATCATGGCCCTCTACCT
GAAGACCAAGGGCGACTTCATCCTGGTGGGCGACCTTATGCGCTCAGTGC
TGCTGCTTGCCTACAAGCCCATGGAAGGAAACTTTGAAGAGATTGCTCGA
GACTTTAATCCCAACTGGATGAGTGCTGTGGAAATCTTGGATGATGACAA
TTTTCTGGGGGCTGAAAATGCCTTTAACTTGTTTGTGTGTCAAAAGGATA
GCGCTGCCACCACTGACGAGGAGCGGCAGCACCTCCAGGAGGTTGGTCTT
TTCCACCTGGGCGAGTTTGTCAATGTCTTTTGCCACGGCTCTCTGGTAAT
GCAGAATCTGGGTGAGACTTCCACCCCCACACAAGGCTCGGTGCTCTTCG
GCACGGTCAACGGCATGATAGGGCTGGTGACCTCACTGTCAGAGAGCTGG
TACAACCTCCTGCTGGACATGCAGAATCGACTCAATAAAGTCATCAAAAG
TGTGGGGAAGATCGAGCACTCCTTCTGGAGATCCTTTCACACCGAGCGGA
AGACAGAACCAGCCACAGGTTTCATCGACGGTGACTTGATTGAGAGTTTC
CTGGATATTAGCCGCCCCAAGATGCAGGAGGTGGTGGCAAACCTACAGTA
TGACGATGGCAGCGGTATGAAGCGAGAGGCCACTGCAGACGACCTCATCA
AGGTTGTGGAGGAGCTAACTCGGATCCATTAGGCGGCCGC

Amino Acid Sequence

MYPYDVPDYAGIPGIPGSTRAAAMSYNYVVTAQKPTAVNGCVTGHFTSAE
DLNLLIAKNTRLEIYVVTAEGLRPVKEVGMYGKIAVMELFRPKGESKDLL
FILTAKYNACILEYKQSGESIDIITRAHGNVQDRIGRPSETGIIGIIDPE
CRMIGLRLYDGLFKVIPLDRDNKELKAFNIRLEELHVIDVKFLYGCQAPT
ICFVYQDPQGRHVKTYEVSLREKEFNKGPWKQENVEAEASMVIAVPEPFG
GAIIIGQESITYHNGDKYLAIAPPIIKQSTIVCHNRVDPNGSRYLLGDME
GRLFMLLLEKEEQMDGTVTLKDLRVELLGETSIAECLTYLDNGVVFVGSR
LGDSQLVKLNVDSNEQGSYVVAMETFTNLGPIVDMCVVDLERQGQGQLVT
CSGAFKEGSLRIIRNGIGIHEHASIDLPGIKGLWPLRSDPNRETYDTLVL
SFVGQTRVLMLNGEEVEETELMGFVDDQQTFFCGNVAHQQLIQITSASVR
LVSQEPKALVSEWKEPQAKNISVASCNSSQVVVAVGRALYYLQIHPQELR
QISHTEMEHEVACLDITPLGDSNGLSPLCAIGLWTDISARILKLPSFELL
HKEMLGGEIIPRSILMTTFESSHYLLCALGDGALFYFGLNIETGLLSDRK
KVTLGTQPTVLRTFRSLSTTNVFACSDRPTVIYSSNHKLVFSNVNLKEVN
YMCPLNSDGYPDSLALANNSTLTIGTIDEIQKLHIRTVPLYESPRKICYQ
EVSQCFGVLSSRIEVQDTSGGTTALRPSASTQALSSSVSSSKLFSSSTAP
HETSFGEEVEVHNLLIIDQHTFEVLHAHQFLQNEYALSLVSCKLGKDPNT
YFIVGTAMVYPEEAEPKQGRIVVFQYSDGKLQTVAEKEVKGAVYSMVEFN
GKLLASINSTVRLYEWTTEKDVRTECNHYNNIMALYLKTKGDFILVGDLM
RSVLLLAYKPMEGNFEEIARDFNPNWMSAVEILDDDNFLGAENAFNLFVC
QKDSAATTDEERQHLQEVGLFHLGEFVNVFCHGSLVMQNLGETSTPTQGS
VLFGTVNGMIGLVTSLSESWYNLLLDMQNRLNKVIKSVGKIEHSFWRSFH
TERKTEPATGFIDGDLIESFLDISRPKMQEVVANLQYDDGSGMKREATAD
DLIKVVEELTRIH*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU8717
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter HA for mammalian expression.; ; Estimated MW of expressed protein : 129349 Daltons