cDNA Clone MEKK3

• Synonyms: MAP3K3
• Plasmid: pIRES3 HA MEKK3
• Parent Plasmid: pIRESpuro3
• Expressed: HA MEKK3
• Tag: HA
• Species: Human

Sequence of Insert

GAATTCGCCACCATGTACCCATACGATGTGCCAGATTACGCCGACGACGA
ACAGGAGGCATTGAACTCAATCATGAACGATCTGGTGGCCCTCCGGATGA
ACCGACGTCACCGGATGCCTGGATATGAGACCATGAAGAACAAAGACACA
GGTCACTCAAATAGGCAGAGTGACGTCAGAATCAAGTTCGAGCACAACGG
GGAGAGGCGAATTATAGCGTTCAGCCGGCCTGTGAAATATGAAGATGTGG
AGCACAAGGTGACAACAGTATTTGGACAACCTCTTGATCTACATTACATG
AACAATGAGCTCTCCATCCTGCTGAAAAACCAAGATGATCTTGATAAAGC
AATTGACATTTTAGATAGAAGCTCAAGCATGAAAAGCCTTAGGATATTGC
TGTTGTCCCAGGACAGAAACCATAACAGTTCCTCTCCCCACTCTGGGGTG
TCCAGACAGGTGCGGATCAAGGCTTCCCAGTCCGCAGGGGATATAAATAC
TATCTACCAGCCCCCCGAGCCCAGAAGCAGGCACCTCTCTGTCAGCTCCC
AGAACCCTGGCCGAAGCTCACCTCCCCCTGGCTATGTTCCTGAGCGGCAG
CAGCACATTGCCCGGCAGGGGTCCTACACCAGCATCAACAGTGAGGGGGA
GTTCATCCCAGAGACCAGCGAGCAGTGCATGCTGGATCCCCTGAGCAGTG
CAGAAAATTCCTTGTCTGGAAGCTGCCAATCCTTGGACAGCCCATCCTTC
CGGAAATCACGAATGTCCCGTGCCCAGAGCTTCCCTGACAACAGACAGGA
ATACTCAGATCGGGAAACTCAGCTTTATGACAAAGGGGTCAAAGGTGGAA
CCTACCCCCGGCGCTACCACGTGTCTGTGCACCACAAGGACTACAGTGAT
GGCAGAAGAACATTTCCCCGAATACGGCGTCATCAAGGCAACTTGTTCAC
CCTGGTGCCCTCCAGCCGCTCCCTGAGCACAAATGGCGAGAACATGGGCC
TGGCTGTGCAATACCTGGACCCCCGTGGGCGCCTGCGGAGTGCGGACAGC
GAGAATGCCCTCTCTGTGCAGGAGAGGAATGTGCCAACCAAGTCTCCCAG
TGCCCCCATCAACTGGCGCCGGGGAAAGCTCCTGGGCCAGGGTGCCTTCG
GCAGGGTCTATTTGTGCTATGACGTGGACACGGGACGTGAACTTGCTTCC
AAGCAGGTCCAATTTGATCCAGACAGTCCTGAGACAAGCAAGGAGGTGAG
TGCTCTGGAGTGCGAGATCCAGTTGCTAAAGAACTTGCAGCATGAGCGCA
TCGTGCAGTACTATGGCTGTCTGCGGGACCGCGCTGAGAAGACCCTGACC
ATCTTCATGGAGTACATGCCAGGGGGCTCGGTGAAAGACCAGTTGAAGGC
TTACGGTGCTCTGACAGAGAGCGTGACCCGAAAGTACACGCGGCAGATCC
TGGAGGGCATGTCCTACCTGCACAGCAACATGATTGTTCACCGGGACATT
AAGGGAGCCAACATCCTCCGAGACTCTGCTGGGAATGTAAAGCTGGGGGA
CTTTGGGGCCAGCAAACGCCTGCAGACGATCTGTATGTCGGGGACGGGCA
TGCGCTCCGTCACTGGCACACCCTACTGGATGAGCCCTGAGGTGATCAGC
GGCGAGGGCTATGGAAGGAAAGCAGACGTGTGGAGCCTGGGCTGCACTGT
GGTGGAGATGCTGACAGAGAAACCACCGTGGGCAGAGTATGAAGCTATGG
CCGCCATCTTCAAGATTGCCACCCAGCCCACCAATCCTCAGCTGCCCTCC
CACATCTCTGAACATGGCCGGGACTTCCTGAGGCGCATTTTTGTGGAGGC
TCGCCAGAGACCTTCAGCTGAGGAGCTGCTCACACACCACTTTGCACAGC
TCATGTACTGAGAATTCGCAAGGGCGAATTC

Amino Acid Sequence

MYPYDVPDYADDEQEALNSIMNDLVALRMNRRHRMPGYETMKNKDTGHSN
RQSDVRIKFEHNGERRIIAFSRPVKYEDVEHKVTTVFGQPLDLHYMNNEL
SILLKNQDDLDKAIDILDRSSSMKSLRILLLSQDRNHNSSSPHSGVSRQV
RIKASQSAGDINTIYQPPEPRSRHLSVSSQNPGRSSPPPGYVPERQQHIA
RQGSYTSINSEGEFIPETSEQCMLDPLSSAENSLSGSCQSLDSPSFRKSR
MSRAQSFPDNRQEYSDRETQLYDKGVKGGTYPRRYHVSVHHKDYSDGRRT
FPRIRRHQGNLFTLVPSSRSLSTNGENMGLAVQYLDPRGRLRSADSENAL
SVQERNVPTKSPSAPINWRRGKLLGQGAFGRVYLCYDVDTGRELASKQVQ
FDPDSPETSKEVSALECEIQLLKNLQHERIVQYYGCLRDRAEKTLTIFME
YMPGGSVKDQLKAYGALTESVTRKYTRQILEGMSYLHSNMIVHRDIKGAN
ILRDSAGNVKLGDFGASKRLQTICMSGTGMRSVTGTPYWMSPEVISGEGY
GRKADVWSLGCTVVEMLTEKPPWAEYEAMAAIFKIATQPTNPQLPSHISE
HGRDFLRRIFVEARQRPSAEELLTHHFAQLMY*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU8584
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter HA for mammalian expression.; ; Estimated MW of expressed protein : 72382 Daltons