cDNA Clone Ubiquitin

• Plasmid: pIRESpuro3 HA Ubiquitin K48R
• Parent Plasmid: pIRESpuro3
• Expressed: HA Ubiquitin K48R
• Genbank: P62988
• Tag: HA

Sequence of Insert

GGATCCGCCACCATGTACCCATACGATGTGCCAGATTACGCCCAGATCTT
CGTGAAGACCCTGACTGGTAAGACCATCACTCTCGAAGTGGAGCCGAGTG
ACACCATTGAGAATGTCAAGGCAAAGATCCAAGACAAGGAAGGCATCCCT
CCTGACCAGCAGAGGTTGATCTTTGCTGGGAGACAGCTGGAAGATGGACG
CACCCTGTCTGACTACAACATCCAGAAAGAGTCCACCCTGCACCTGGTCC
TCCGTCTCAGAGGTGGGTGATAAGGATCC

Amino Acid Sequence

MYPYDVPDYAQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQ
RLIFAGRQLEDGRTLSDYNIQKESTLHLVLRLRGG*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU8413
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal HA tag for mammalian expression.