cDNA Clone VPS15

• Plasmid: pCMV-HA VPS15 1-680
• Parent Plasmid: pCMV5 HA2
• Expressed: HA-VPS15 1-680
• Genbank: NP_055417
• Tag: HA
• Species: Human

Sequence of Insert

GCGGCCGCGATGGGAAATCAGCTTGCTGGCATTGCTCCCTCCCAGATCCT
TTCTGTAGAGAGTTATTTTTCAGATATTCATGACTTTGAATATGATAAAA
GCCTGGGGAGTACTCGGTTTTTTAAAGTTGCTCGAGCCAAGCACCGAGAA
GGCCTGGTCGTTGTGAAGGTTTTTGCAATTCAGGATCCCACATTGCCTTT
AACCAGCTATAAACAAGAGCTGGAGGAACTGAAAATCAGGCTTAATTCTG
CACAGAATTGTCTACCTTTCCAGAAAGCATCAGAAAAAGCATCTGAGAAA
GCAGCTATGCTCTTTAGGCAGTATGTGCGAGACAATCTCTATGATCGCAT
CAGTACCCGTCCATTCTTGAATAACATTGAGAAGCGCTGGATTGCTTTCC
AGATCCTGACAGCTGTGGACCAAGCACACAAATCTGGAGTTCGTCATGGG
GACATCAAGACTGAGAATGTGATGGTCACCAGTTGGAATTGGGTTCTTCT
AACTGATTTTGCCAGTTTTAAGCCCACTTATCTTCCAGAAGACAACCCGG
CAGATTTCAATTATTTCTTTGACACATCACGGAGGAGAACTTGCTATATT
GCTCCTGAACGTTTTGTTGATGGTGGGATGTTTGCCACTGAGTTAGAATA
TATGAGAGATCCTTCAACTCCGCTTGTAGACTTAAATAGCAATCAGAGAA
CAAGAGGAGAGTTGAAGAGAGCAATGGACATCTTTTCAGCAGGTTGTGTG
ATAGCTGAGCTTTTTACAGAAGGTGTACCATTATTTGATCTCTCTCAACT
TTTGGCTTATAGAAATGGACATTTTTTCCCTGAACAAGTGCTAAATAAAA
TTGAAGATCACAGTATCAGAGAATTGGTAACTCAGATGATTCACCGTGAG
CCAGATAAACGTTTAGAGGCAGAAGATTACTTAAAACAGCAGCGTGGCAA
TGCCTTTCCTGAAATATTTTACACTTTTCTTCAGCCCTACATGGCCCAGT
TTGCCAAGGAAACGTTTCTTTCTGCAGATGAGCGTATTCTGGTTATACGG
AAGGATTTGGGCAACATTATTCACAATCTCTGTGGACATGATCTGCCAGA
AAAAGCCGAAGGAGAGCCTAAGGAAAATGGGCTGGTTATCTTGGTATCTG
TTATAACATCCTGCCTACAGACCCTTAAATACTGTGATTCCAAACTAGCT
GCTTTGGAACTGATTCTTCATTTGGCTCCAAGATTAAGTGTTGAAATCCT
TTTGGATCGTATTACTCCATATCTTTTGCATTTCAGCAATGACTCTGTTC
CTAGGGTGAGGGCTGAAGCCTTGAGGACGTTGACCAAAGTTCTTGCTCTC
GTCAAAGAGGTTCCTCGTAATGATATCAATATTTATCCGGAATACATTCT
GCCAGGCATAGCCCACTTAGCCCAAGATGATGCTACTATCGTTAGACTAG
CCTATGCTGAAAACATAGCTCTGCTGGCAGAAACAGCTCTGAGATTCCTG
GAATTAGTACAGTTAAAAAATCTTAATATGGAAAATGACCCCAATAATGA
AGAAATAGATGAGGTTACACATCCAAATGGAAATTATGACACAGAGCTCC
AAGCCTTACATGAAATGGTCCAGCAGAAAGTTGTTACTTTGCTAAGTGAC
CCTGAAAATATTGTAAAACAAACCTTGATGGAAAATGGAATAACACGGCT
GTGTGTATTCTTTGGACGTCAGAAAGCCAACGATGTTTTGTTGTCCCACA
TGATTACTTTCCTAAATGATAAGAATGATTGGCATCTACGTGGAGCATTT
TTTGATAGTATAGTTGGTGTTGCTGCCTATGTTGGCTGGCAAAGCTCCTC
AATTCTCAAGCCTCTGCTGCAACAAGGTCTTAGTGATGCTGAGGAATTTG
TCATTGTGAAAGCTCTTTATGCCCTTACTTGTATGTGCCAGTTAGGACTG
CTACAAAAACCCCATGTTTACGAATTTGCCAGTGATATTGCCCCCTTCCT
GTGTCATCCCAATTTATGGATACGTTATGGTGCCGTGGGATTTATCACAT
AAGCGGCCGC

Amino Acid Sequence

MYPYDVPDYAGIPGIPGSTRAAAMGNQLAGIAPSQILSVESYFSDIHDFE
YDKSLGSTRFFKVARAKHREGLVVVKVFAIQDPTLPLTSYKQELEELKIR
LNSAQNCLPFQKASEKASEKAAMLFRQYVRDNLYDRISTRPFLNNIEKRW
IAFQILTAVDQAHKSGVRHGDIKTENVMVTSWNWVLLTDFASFKPTYLPE
DNPADFNYFFDTSRRRTCYIAPERFVDGGMFATELEYMRDPSTPLVDLNS
NQRTRGELKRAMDIFSAGCVIAELFTEGVPLFDLSQLLAYRNGHFFPEQV
LNKIEDHSIRELVTQMIHREPDKRLEAEDYLKQQRGNAFPEIFYTFLQPY
MAQFAKETFLSADERILVIRKDLGNIIHNLCGHDLPEKAEGEPKENGLVI
LVSVITSCLQTLKYCDSKLAALELILHLAPRLSVEILLDRITPYLLHFSN
DSVPRVRAEALRTLTKVLALVKEVPRNDINIYPEYILPGIAHLAQDDATI
VRLAYAENIALLAETALRFLELVQLKNLNMENDPNNEEIDEVTHPNGNYD
TELQALHEMVQQKVVTLLSDPENIVKQTLMENGITRLCVFFGRQKANDVL
LSHMITFLNDKNDWHLRGAFFDSIVGVAAYVGWQSSSILKPLLQQGLSDA
EEFVIVKALYALTCMCQLGLLQKPHVYEFASDIAPFLCHPNLWIRYGAVG
FIT*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU8384
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter HA for mammalian expression.; ; Estimated MW of expressed protein : 79983 Daltons