cDNA Clone LCB1

• Plasmid: pCMV-HA-1 LCB1
• Parent Plasmid: pCMV5 HA1
• Genbank: BAC40709
• Tag: HA
• Species: Mouse

Sequence of Insert

GGATCCATGGCGACAGTGGCGGAGCAGTGGGTGCTGGTGGAGATGGTGCA
GGCGCTGTACGAGGCTCCAGCATACCATCTTATTTTGGAAGGAATCCTAA
TACTTTGGATAATCAGACTCGTTTTCTCTAAAACTTACAAGTTGCAGGAG
CGTTCTGATCTTACAGCCAAGGAAAAGGAAGAACTGATTGAAGAGTGGCA
GCCAGAGCCCCTCGTCCCTCCAGTCTCCAAGAACCACCCTGCTCTCAACT
ACAACATCGTGTCCGGCCCTCCAACCCACAACATCGTGGTGAATGGAAAA
GAGTGTGTCAACTTTGCCTCCTTTAACTTCCTTGGGCTGCTGGCCAACCC
TCGAGTTAAGGCCGCAGCTTTTTCATCTTTAAAGAAGTACGGAGTGGGTA
CCTGTGGTCCTCGAGGGTTCTATGGCACATTTGATGTCCATCTGGATTTA
GAAGAGCGCCTGGCAAAGTTTATGAAGACCGAAGAAGCCATCATTTACTC
GTATGGCTTCTCCACCATAGCCAGTGCCATTCCTGCGTACTCTAAGAGAG
GGGACATCATCTTTGTGGACAGTGCGGCCTGCTTTGCTATCCAGAAAGGA
TTACAGGCATCAAGAAGTGACATTAAGTTATTTAAGCATAATGATGTGGC
TGACCTGGAGCGACTGCTAAAAGAACAAGAGATTGAAGATCAAAAGAATC
CTCGCAAGGCCCGTGTGACTCGGCGGTTCATCGTGGTGGAGGGCTTGTAC
ATGAACACTGGAACCATCTGTCCCCTTCCAGAACTGGTTAAATTAAAATA
TAAATATAAAGCAAGAATATTCCTGGAGGAGAGCCTGTCCTTTGGAGTCC
TTGGGGAGCACGGGCGAGGAGTCACCGAGCACTATGGGATCAGTATTGAT
GATATTGACCTTATCAGTGCTAACATGGAGAATGCACTCGCTTCTGTCGG
GGGCTTCTGCTGTGGCCGCTCCTTCGTGGTTGACCATCAGCGGCTCTCCG
GTCAAGGATACTGCTTTTCTGCTTCGTTACCTCCCCTGCTAGCTGCTGCT
GCCATTGAGGCCCTCAACATCATGGAAGAGAATCCAGATATTTTTGCAGT
TTTAAAGAAAAAATGCCAGAACATCCATAAGTCTCTACAAGGTGTTTCGG
GTTTAAAAGTGGTGGGAGAGTCCCTTTCTCCGGCGCTTCATCTCCAGCTG
GAAGAGAGCACGGGCTCTCGGGAGAAAGATGTGAAGCTGCTTCAAGCAAT
CGTAGATCAGTGCATGGATAAGGGCATCGCATTGACTCAGGCACGGTACT
TGGACAAGGAAGAGAAGTGCCTTCCTCCTCCAAGCATCAGGGTTGTGGTC
ACTGTGGAGCAGACGGAGGAAGAGCTACAGAGGGCTGCATCCACCATCAG
GGAGGCGGCCCAGGCTGTGCTGTTGTAGGCGGCCGC

Amino Acid Sequence

MYPYDVPDYAGSMATVAEQWVLVEMVQALYEAPAYHLILEGILILWIIRL
VFSKTYKLQERSDLTAKEKEELIEEWQPEPLVPPVSKNHPALNYNIVSGP
PTHNIVVNGKECVNFASFNFLGLLANPRVKAAAFSSLKKYGVGTCGPRGF
YGTFDVHLDLEERLAKFMKTEEAIIYSYGFSTIASAIPAYSKRGDIIFVD
SAACFAIQKGLQASRSDIKLFKHNDVADLERLLKEQEIEDQKNPRKARVT
RRFIVVEGLYMNTGTICPLPELVKLKYKYKARIFLEESLSFGVLGEHGRG
VTEHYGISIDDIDLISANMENALASVGGFCCGRSFVVDHQRLSGQGYCFS
ASLPPLLAAAAIEALNIMEENPDIFAVLKKKCQNIHKSLQGVSGLKVVGE
SLSPALHLQLEESTGSREKDVKLLQAIVDQCMDKGIALTQARYLDKEEKC
LPPPSIRVVVTVEQTEEELQRAASTIREAAQAVLL*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU8088
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal HA for mammalian expression.; Estimated MW of expressed protein : 53829 Daltons