cDNA Clone LCB2 (mouse)

• Plasmid: pCMV-HA-1 LCB2
• Parent Plasmid: pCMV5 HA1
• Genbank: AAC53310
• Tag: HA
• Species: Mouse

Sequence of Insert

GAATTCATGCGGCCGGAGCCCGGAGGCTGCTGCTGCCGCCGCCCGATGCG
GGCGAACGGCTGCGTCAAGAACGGGGAAGTGAGGAACGGGTACTTGAGGA
GCAGCACCGCCACCGTCGCGGCTGCCGGCCAGATTCATCATGTAACAGAA
AATGGAGGACTGTACAAAAGACCGTTTAATGAAGCTTTTGAAGAAACACC
CATGCTGGTTGCTGTGCTCACATATGTGGGCTATGGCGTACTCACCCTCT
TTGGATATCTTCGAGATTTCTTGAGGCATTGGAGAATTGAAAAGTGCCAC
CATGCAACAGAAAGAGAAGAACAAAAGGACTTTGTGTCCTTGTATCAGGA
TTTTGAAAACTTCTATACAAGGAACCTCTACATGAGAATCAGAGACAACT
GGAATCGACCTATCTGTAGTGTGCCTGGAGCCAAGGTGGATATCATGGAG
AGAAAATCTCATGACTATAACTGGTCATTCAAGTACACAGGGAATATAAT
TAAAGGTGTAATAAACATGGGTTCCTACAACTATCTTGGATTTGCGAGGA
ACACTGGATCATGTCAGGAAGCAGCTGCTGAAGTCCTCAAGGAGTATGGA
GCAGGGGTGTGCAGCACTCGTCAGGAAATTGGAAACCTGGACAAGCATGA
AGAACTAGAGAAATTGGTAGCAAGGTTCTTAGGTGTGGAAGCTGCTATGA
CCTATGGCATGGGATTTGCAACAAATTCAATGAACATTCCTGCTCTTGTT
GGCAAAGGTTGCCTGATTCTGAGTGATGAGCTGAACCATGCGTCACTGGT
TCTAGGAGCCAGACTGTCAGGAGCAACCATTCGAATCTTCAAACACAACA
ATATGCAAAGCTTAGAGAAGCTTTTAAAAGATGCCATTGTTTATGGTCAG
CCTCGGACAAGAAGACCCTGGAAGAAAATCTTAATCCTTGTGGAAGGCAT
ATATAGTATGGAGGGGTCTATTGTTCGCCTTCCTGAAGTGATTGCTCTCA
AGAAGAAATACAAGGCATACTTGTATCTGGATGAGGCTCACAGCATTGGG
GCTCTTGGCCCTTCAGGGCGAGGCGTGGTAGATTACTTCGGCCTGGATCC
TGAGGATGTAGATGTTATGATGGGAACATTCACAAAGAGCTTCGGTGCTT
CAGGAGGATACATCGGAGGCAAGAAGGAGCTGATAGACTACCTGCGCACA
CATTCTCACAGTGCTGTGTATGCCACGTCGATGTCACCGCCTGTGATGGA
ACAGATTATCACCTCCATGAAGTGCATCATGGGGCAGGATGGCACCAGTC
TTGGCAAAGAATGTATACAGCAGTTGGCTGAGAACACCAGGTATTTCAGG
AGACGCCTGAAGGAAATGGGGTTCATCATCTATGGCAATGAAGACTCCCC
GGTGGTGCCTTTGATGCTCTACATGCCGGCCAAAATTGGCGCCTTTGGAA
GAGAGATGCTGAAGCGGAACATTGGTGTAGTTGTGGTGGGATTTCCTGCT
ACCCCGATCATTGAGTCCAGAGCCAGATTTTGCCTGTCAGCAGCTCATAC
CAAAGAAATACTTGACACTGCTTTGAAGGAGATAGATGAAGTTGGGGATC
TGCTGCAGCTAAAGTACTCTCGCCACCGGCTGGTGCCTCTACTGGACAGG
CCCTTTGATGAGACTACCTATGAAGAGACAGAAGACTGAGTCGAC

Amino Acid Sequence

MYPYDVPDYAGSPEFMRPEPGGCCCRRPMRANGCVKNGEVRNGYLRSSTA
TVAAAGQIHHVTENGGLYKRPFNEAFEETPMLVAVLTYVGYGVLTLFGYL
RDFLRHWRIEKCHHATEREEQKDFVSLYQDFENFYTRNLYMRIRDNWNRP
ICSVPGAKVDIMERKSHDYNWSFKYTGNIIKGVINMGSYNYLGFARNTGS
CQEAAAEVLKEYGAGVCSTRQEIGNLDKHEELEKLVARFLGVEAAMTYGM
GFATNSMNIPALVGKGCLILSDELNHASLVLGARLSGATIRIFKHNNMQS
LEKLLKDAIVYGQPRTRRPWKKILILVEGIYSMEGSIVRLPEVIALKKKY
KAYLYLDEAHSIGALGPSGRGVVDYFGLDPEDVDVMMGTFTKSFGASGGY
IGGKKELIDYLRTHSHSAVYATSMSPPVMEQIITSMKCIMGQDGTSLGKE
CIQQLAENTRYFRRRLKEMGFIIYGNEDSPVVPLMLYMPAKIGAFGREML
KRNIGVVVVGFPATPIIESRARFCLSAAHTKEILDTALKEIDEVGDLLQL
KYSRHRLVPLLDRPFDETTYEETED*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU8064
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal HA for mammalian expression.; Estimated MW of expressed protein : 64672 Daltons