cDNA Clone FGD6

• Plasmid: pEBG6P-1 FGD6
• Parent Plasmid: pEBG6P
• Expressed: GST-FGD6
• Genbank: NM_018351
• Tag: GST
• Species: Human

Sequence of Insert

gcggccgcgatgacttctgcagccgagataaagaagccaccagtggcccccaagcccaagtttgttgtggcaaataacaagccagccccacctcctattgcacctaaacccgacattgtgatttctagtgttccacagtcgacaaagaaaatgaaaccagcaatagccccaaaaccaaaagtcctgaagacctcacctgttcgagagattgggcagtcgccatcaaggaaaatcatgttgaacctggaagggcataaacaggaattagctgaaagcactgacaactttaattgtaaatatgaaggcaatcagagcaatgattatatttcaccaatgtgttcctgcagttctgagtgtatccataagctgggccatagagagaatttgtgtgtaaagcagcttgtcttagagcccctggaaatgaatgaaaatttagaaaacagtaaaattgatgagact
ttgactataaaaactaggagtaaatgtgatttgtatggtgaaaaagccaa
gaaccagggtggagttgttttaaaggcaagcgttttagaagaggagctca
aagatgccttaatacaccaaatgccaccttttatttctgcacagaagcac
aggcccacagacagcccagaaatgaatggtggctgtaattcaaatggaca
attcagaattgaatttgcggatttgtcaccttccccatccagctttgaaa
aagttcctgatcatcacagttgccacttacagcttcctagtgatgaatgt
gaacattttgaaacttgccaggatgacagtgaaaaaagcaataattgctt
tcagtcatctgaactagaggctctggaaaatgggaaaaggagtactttaa
tatcttcagatggagttagtaagaaatcagaagtcaaagaccttggtccc
ttagaaattcatttagtaccatataccccaaaatttccaactcccaagcc
cagaaagacacgaactgctcgtctgttacgccaaaagtgtgtagatactc
ctagtgaaagcactgaagaaccggggaattcagacagtagctcttcctgt
cttactgaaaatagtttgaaaatcaataaaatcagtgttctgcatcagaa
tgttttgtgtaagcaggaacaggtggataaaatgaagctaggaaataaaa
gtgaattgaatatggaatccaacagtgatgcacaggacttagtcaattca
cagaaagccatgtgtaatgaaacaacttcctttgaaaaaatggcaccttc
ttttgataaagactctaatttgagttctgacagcacaactgtagatggtt
ctagtatgtcgcttgctgtggacgaagggaccggttttataagatgtact
gtatctatgagcctgcctaagcagctcaaattaacttgcaatgaacattt
gcaatctgggagaaacctgggagtttctgcccctcaaatgcaaaaggaat
ctgttataaaagaggaaaattctctacgaattgtccccaaaaaacctcaa
agacatagcttgcctgctacaggagtgcttaaaaaggctgcctccgagga
gcttttggaaaaaagttcttatccttcaagtgaagaaaaaagttcagaga
agagtctagaaagaaatcaccttcagcatttgtgtgcccaaaaccgtggt
gtgtcatcctcctttgatatgcctaaacgggcttcagaaaagccagtgtg
gaagttacctcatcctattttacccttttcagggaacccagaattcttaa
agtctgtcaccgtatcgtcaaacagtgagccttcaacagccctaaccaag
cccagagcaaaatcgttatctgctatggatgtggaaaagtgcactaagcc
ttgcaaagactctacaaagaaaaactcttttaaaaagttgctcagcatga
aactgtccatctgtttcatgaagagtgactttcaaaaattttggtccaag
agtagccaacttggagacaccaccacaggccacctctccagtggggagca
gaaggggattgaaagtgattggcaaggcttgttggtaggagaggagaaga
gaagtaaacccatcaaggcatattccacagaaaactatagcctggaatct
caaaagaagaggaagaagtctcggggccagaccagtgcagctaatggtct
gagagctgagtctttggatgaccaaatgctctcccgggagtcatcatctc
aggcaccttacaagtctgttacaagcctctgtgcaccggagtatgaaaat
atacgccattatgaggaaataccagagtacgagaacttgccatttattat
ggctatacggaaaactcaagagttggaatggcagaattccagcagcatgg
aggacgctgatgcaaatgtgtatgaggtagaagagccgtatgaagctcca
gatggccagctgcagcttggacccagacatcagcattccagttcaggagc
atcccaggaggaacagaatgatcttggtcttggtgaccttccctctgatg
aggaggaaatcatcaacagttctgatgaagatgatgtcagctctgagtca
agtaaaggagagcctgacccactggaagataaacaggatgaagataatgg
aatgaaaagtaaagttcatcatattgccaaggagatcatgagctcagaga
aagtgtttgtggatgtgttaaaacttttgcatattgatttccgggatgca
gtagctcatgcttccaggcaacttgggaaaccagtgattgaggaccggat
tctaaatcagatcctatactacttgcctcagctgtatgagctcaaccggg
atctcttgaaggaactggaggaaagaatgttgcactggactgaacaccaa
agaattgctgatatctttgtaaagaagggaccatatctaaaaatgtattc
cacatacatcaaagaatttgataagaatatagccttgctggatgaacagt
gcaagaaaaatccaggttttgctgctgttgttagagaatttgagatgagc
cctcgctgtgctaatctggccctcaagcactacctgctcaagccggttca
gaggatcccccagtacaggctgttgctgacagattatttgaagaatctca
tagaagatgctggagattacagagacactcaagatgcccttgctgttgtt
atagaggtagccaaccacgccaatgacaccatgaagcaaggagacaactt
tcagaaacttatgcaaattcagtacagcttaaatggacaccatgaaattg
tgcagcctggtcgggtttttctcaaagaaggaattctgatgaagctgtct
cggaaagtgatgcaacctcgaatgtttttcctgtttaatgatgccctgct
gtatacaacaccagtgcagtctgggatgtataaactgaacaacatgctct
cactggctggaatgaaggtcagaaaacctacccaagaagcctatcagaat
gaattaaagattgaaagtgtagaacgttccttcattctctcagccagttc
tgccacagaaagggatgaatggctagaagcgatttccagggcaatagaag
agtatgccaagaaaagaatcaccttctgtcctagtaggagtcttgatgag
gcagactcagaaaataaagaagaagttagtcctcttggatcgaaggctcc
catctggattcctgataccagagccacaatgtgtatgatctgcacaagcg
aattcactctcacctggagacgacaccactgccgggcctgtggaaagatt
gtatgccaagcttgttcgtctaataagtatggcttagattacctgaaaaa
tcaaccagcaagagtatgtgaacattgtttccaagaactgcagaaattag
atcaccagcactcccctaggattggatctcctggaaatcacaaatctcct
tcaagtgccttatcatcagtcttacatagcattccatcagggaggaaaca
gaaaaaaatcccagctgctctcaaagaagtatcagcaaacacagaggatt
cttctatgagtggctacttgtacagatcaaagggcaataaaaaaccctgg
aaacacttttggtttgtcataaaaaataaagtactatatacatatgctgc
aagtgaggacgtggccgctttggagagtcagcctttattaggattcactg
ttattcaagttaaagatgagaattccgagtctaaagtatttcagttactg
cacaaaaacatgttattttatgtattcaaagcagaggatgctcactcggc
tcagaagtggatagaagcatttcaggaaggcacaatattgtaggcggccgc

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSTSGTIDAAAMTSA
AEIKKPPVAPKPKFVVANNKPAPPPIAPKPDIVISSVPQSTKKMKPAIAP
KPKVLKTSPVREIGQSPSRKIMLNLEGHKQELAESTDNFNCKYEGNQSND
YISPMCSCSSECIHKLGHRENLCVKQLVLEPLEMNENLENSKIDETLTIK
TRSKCDLYGEKAKNQGGVVLKASVLEEELKDALIHQMPPFISAQKHRPTD
SPEMNGGCNSNGQFRIEFADLSPSPSSFEKVPDHHSCHLQLPSDECEHFE
TCQDDSEKSNNCFQSSELEALENGKRSTLISSDGVSKKSEVKDLGPLEIH
LVPYTPKFPTPKPRKTRTARLLRQKCVDTPSESTEEPGNSDSSSSCLTEN
SLKINKISVLHQNVLCKQEQVDKMKLGNKSELNMESNSDAQDLVNSQKAM
CNETTSFEKMAPSFDKDSNLSSDSTTVDGSSMSLAVDEGTGFIRCTVSMS
LPKQLKLTCNEHLQSGRNLGVSAPQMQKESVIKEENSLRIVPKKPQRHSL
PATGVLKKAASEELLEKSSYPSSEEKSSEKSLERNHLQHLCAQNRGVSSS
FDMPKRASEKPVWKLPHPILPFSGNPEFLKSVTVSSNSEPSTALTKPRAK
SLSAMDVEKCTKPCKDSTKKNSFKKLLSMKLSICFMKSDFQKFWSKSSQL
GDTTTGHLSSGEQKGIESDWQGLLVGEEKRSKPIKAYSTENYSLESQKKR
KKSRGQTSAANGLRAESLDDQMLSRESSSQAPYKSVTSLCAPEYENIRHY
EEIPEYENLPFIMAIRKTQELEWQNSSSMEDADANVYEVEEPYEAPDGQL
QLGPRHQHSSSGASQEEQNDLGLGDLPSDEEEIINSSDEDDVSSESSKGE
PDPLEDKQDEDNGMKSKVHHIAKEIMSSEKVFVDVLKLLHIDFRDAVAHA
SRQLGKPVIEDRILNQILYYLPQLYELNRDLLKELEERMLHWTEHQRIAD
IFVKKGPYLKMYSTYIKEFDKNIALLDEQCKKNPGFAAVVREFEMSPRCA
NLALKHYLLKPVQRIPQYRLLLTDYLKNLIEDAGDYRDTQDALAVVIEVA
NHANDTMKQGDNFQKLMQIQYSLNGHHEIVQPGRVFLKEGILMKLSRKVM
QPRMFFLFNDALLYTTPVQSGMYKLNNMLSLAGMKVRKPTQEAYQNELKI
ESVERSFILSASSATERDEWLEAISRAIEEYAKKRITFCPSRSLDEADSE
NKEEVSPLGSKAPIWIPDTRATMCMICTSEFTLTWRRHHCRACGKIVCQA
CSSNKYGLDYLKNQPARVCEHCFQELQKLDHQHSPRIGSPGNHKSPSSAL
SSVLHSIPSGRKQKKIPAALKEVSANTEDSSMSGYLYRSKGNKKPWKHFW
FVIKNKVLYTYAASEDVAALESQPLLGFTVIQVKDENSESKVFQLLHKNM
LFYVFKAEDAHSAQKWIEAFQEGTIL*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU5602
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression plasmid to express FGD6 with N-terminal GST (contains preScission protease site); N.B. sequence differences (all silent) compared with NM_018351:; t69c(N23), t396c(V132), g513a(G171), c1962t(L654), a2388g(P796), t2847c(H949), g2850a(Q950), t4245c(H1415); ; Estimated MW of expressed protein : 189025 Daltons