cDNA Clone MAST2

• Plasmid: pGEX6P-1-MAST2 430-1138
• Parent Plasmid: pGEX6P1
• Expressed: GST-MAST2 430-1138
• Genbank: NM_015112
• Tag: GST
• Species: Human

Sequence of Insert

ATGTCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCAC
TCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATG
AGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTG
GAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACA
GTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTG
GTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTG
GATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAAC
TCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCG
AAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCAT
CCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCC
AATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTG
AAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCA
TGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCC
AAAATCGGATCTGGAAGTTCTGTTCCAGGGGCCCCTGGGATCTCTGGAGT
TTGACCCTGAAGAGTTCTACCACCTTTTAGAAGCAGCTGAGGGCCACGCC
AAGGAGGGACAAGGGATTAAATGTGACATTCCCCGCTACATCGTTAGCCA
GCTGGGCCTCACCCGGGATCCCCTAGAAGAAATGGCCCAGTTGAGCAGCT
GTGACAGTCCTGACACTCCAGAGACAGATGATTCTATTGAGGGCCATGGG
GCATCTCTGCCATCTAAAAAGACACCCTCTGAAGAGGACTTCGAGACCAT
TAAGCTCATCAGCAATGGCGCCTATGGGGCTGTATTTCTGGTGCGGCACA
AGTCCACCCGGCAGCGCTTTGCCATGAAGAAGATCAACAAGCAGAACCTG
ATCCTACGGAACCAGATCCAGCAGGCCTTCGTGGAGCGTGATATACTGAC
TTTCGCTGAGAACCCCTTTGTGGTCAGCATGTTCTGCTCCTTTGATACCA
AGCGCCACTTGTGCATGGTGATGGAGTACGTTGAAGGGGGAGACTGTGCC
ACTCTGCTGAAGAATATTGGGGCCCTGCCTGTGGACATGGTGCGTCTATA
CTTTGCGGAAACTGTGCTGGCCCTGGAGTACTTACACAACTATGGCATCG
TGCACCGTGACCTCAAGCCTGACAACCTCCTAATTACATCCATGGGGCAC
ATCAAGCTCACGGACTTTGGACTGTCCAAAATGGGCCTCATGAGTCTGAC
AACGAACTTGTATGAGGGTCATATTGAAAAGGATGCCCGGGAATTCCTGG
ACAAGCAGGTATGCGGGACCCCAGAATACATTGCGCCTGAGGTGATCCTG
CGCCAGGGCTATGGGAAGCCAGTGGACTGGTGGGCCATGGGCATTATCCT
GTATGAGTTCCTGGTGGGCTGCGTCCCTTTTTTTGGAGATACTCCGGAGG
AGCTCTTTGGGCAGGTGATCAGTGATGAGATTGTGTGGCCTGAGGGTGAT
GAGGCACTGCCCCCAGACGCCCAGGACCTCACCTCCAAACTGCTCCACCA
GAACCCTCTGGAGAGACTTGGCACAGGCAGTGCCTATGAGGTGAAGCAGC
ACCCATTCTTTACTGGTCTGGACTGGACAGGACTTCTCCGCCAGAAGGCT
GAATTTATTCCTCAGTTGGAGTCAGAGGATGATACTAGCTATTTTGACAC
CCGCTCAGAGCGATACCACCACATGGACTCGGAGGATGAGGAAGAAGTGA
GTGAGGATGGCTGCCTTGAGATCCGCCAGTTCTCTTCCTGCTCTCCAAGG
TTCAACAAGGTGTACAGCAGCATGGAGCGGCTCTCACTGCTCGAGGAGCG
CCGGACACCACCCCCGACCAAGCGCAGCCTGAGTGAGGAGAAGGAGGACC
ATTCAGATGGCCTGGCAGGGCTCAAAGGCCGAGACCGGAGCTGGGTGATT
GGCTCCCCTGAGATATTACGGAAGCGGCTGTCGGTGTCTGAGTCGTCCCA
CACAGAGAGTGACTCAAGCCCTCCAATGACAGTGCGACGCCGCTGCTCAG
GCCTCCTGGATGCGCCTCGGTTCCCGGAGGGCCCTGAGGAGGCCAGCAGC
ACCCTCAGGAGGCAACCACAGGAGGGTATATGGGTCCTGACACCCCCATC
TGGAGAGGGGGTATCTGGGCCTGTCACTGAACACTCAGGGGAGCAGCGGC
CAAAGCTGGATGAGGAAGCTGTTGGCCGGAGCAGTGGTTCCAGTCCAGCT
ATGGAGACCCGAGGCCGTGGGACCTCACAGCTGGCTGAGGGAGCCACAGC
CAAGGCCATCAGTGACCTGGCTGTGCGTAGGGCCCGCCACCGGCTGCTCT
CTGGGGACTCAACAGAGAAGCGCACTGCTCGCCCTGTCAACAAAGTGATC
AAGTCCGCCTCAGCCACAGCCCTCTCACTCCTCATTCCTTCGGAACACCA
CACCTGCTCCCCGTTGGCCAGCCCCATGTCCCCACATTCTCAGTCGTCCA
ACCCATCATCCCGGGACTCTTCTCCAAGCAGGGACTTCTTGCCAGCCCTT
GGCAGCATGAGGCCTCCCATCATCATCCACCGAGCTGGCAAGAAGTATGG
CTTCACCCTGCGGGCCATTCGCGTCTACATGGGTGACTCCGATGTCTACA
CCGTGCACCATATGGTGTGGTGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSLEFDPEEFYHLLEAAEGHA
KEGQGIKCDIPRYIVSQLGLTRDPLEEMAQLSSCDSPDTPETDDSIEGHG
ASLPSKKTPSEEDFETIKLISNGAYGAVFLVRHKSTRQRFAMKKINKQNL
ILRNQIQQAFVERDILTFAENPFVVSMFCSFDTKRHLCMVMEYVEGGDCA
TLLKNIGALPVDMVRLYFAETVLALEYLHNYGIVHRDLKPDNLLITSMGH
IKLTDFGLSKMGLMSLTTNLYEGHIEKDAREFLDKQVCGTPEYIAPEVIL
RQGYGKPVDWWAMGIILYEFLVGCVPFFGDTPEELFGQVISDEIVWPEGD
EALPPDAQDLTSKLLHQNPLERLGTGSAYEVKQHPFFTGLDWTGLLRQKA
EFIPQLESEDDTSYFDTRSERYHHMDSEDEEEVSEDGCLEIRQFSSCSPR
FNKVYSSMERLSLLEERRTPPPTKRSLSEEKEDHSDGLAGLKGRDRSWVI
GSPEILRKRLSVSESSHTESDSSPPMTVRRRCSGLLDAPRFPEGPEEASS
TLRRQPQEGIWVLTPPSGEGVSGPVTEHSGEQRPKLDEEAVGRSSGSSPA
METRGRGTSQLAEGATAKAISDLAVRRARHRLLSGDSTEKRTARPVNKVI
KSASATALSLLIPSEHHTCSPLASPMSPHSQSSNPSSRDSSPSRDFLPAL
GSMRPPIIIHRAGKKYGFTLRAIRVYMGDSDVYTVHHMVW*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU4346
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression with N terminal GST tag.