cDNA Clone SLX4 mouse

• Plasmid: pBabepuro DU mSLX4 L355A W356A S359A
• Parent Plasmid: pBabeD puro
• Expressed: mSLX4 L355A W356A S359A
• Genbank: NM_177472.4

Sequence of Insert

ATGGTTCCAGAGAGTGCTCCCAATGGCAACTCCCAGCCTCTTCCTTCCTGTTTCACGACAACTGGTGTACCAAGTCCCTCCAAACCCAGAGTCTCAGAGCTGGTTCTGCAACGGATGAAGCAGTTCAAGAGAGCAGACCCTGAGCGCCTGAGACACGCCTCAGAAGAATCCCCCCAAAAGACTGCACTGGGAGACGATGTCCCAAGGAGCCCTCCAGAGGAGACAGTGGGAGAAAATGAGTATAAACTTGATGCCACAGACAGTGATGCTGCAATGGCCCTGGCCTTACAGCAGGAGTTTAGAAGGGAGGAAGCATCCTCACACCATGACAGCCTGGAGGAGAAGGGGTTGTTCTTCTGTCAAATGTGTCAGAAAAACCTCTCAGCAATGAATGTAACCCGGAGGGAGCAACATGTGAACAGATGCCTAGATGAGGCAGAGAAGGCACAGAGACCTGCTTCACCTCGGATCCCTGATTGCCCAATATGTGGGAAACCGTTCCTCACCACCAAGAGCCGAATCAGTCACTTGAAGCAGTGTGCGGTGAGGATGGAAGTGGGTCCCCAACTCCTGCTTCAGGCTGTGAGGCTACAGACAGCTCAGCCAGAGGTGGATGGCAGCCCTCAGGTACCCAGTTTCAGCAACAACGTTGGAGGTTTGAAACGGAAAGGAGTCACCACCAAAAGGGAGCCAAGGAGGAGGAAAGTCAATAAGCCTGAGGCTCCGTCTGAAGATCTGTTGGTGGCCATGGCTCTGTCTCGCTCTGAGGTGGAGCACTGTCCAGTTGTGCCCCCGCTCAGACTGGAAAATGCTTTTTCTGAGAAGATAAGGCTAGGAGCAGAGAAGAAAAGTCGCAAGAAGAGACCCCCGGTGTGTCCCCCACAGTTGGTAACCCAAGACTCTGAGACCACAGGTAGACAGATTGAAGATCGTGTTGCCCAGCTTCTGTCAGAGGAAGCAGAACTATCTTGCACCCCACCACTTCTTGCCAGCAAGATTTCTAAGGAAGAATTGGAACCTGCAGGCTGGCGAGCACGGCTGCCTGAAGGGAAGCGGAACTTTgcGgcGGAACTCgcCGCTCTCACAGGAGCCTGGGCTGAGGAGTCCTTCTACACAGTGGGACTGTTTCCTCCAATTGTGTCCCAATGCCCTTCCAAGGAACCCCAGCTACCATTGGAGCTGCCCAAGCAAGGAGAGCCGAGTCCACGGCGGCCACCTGCTTCCCAGAGCAGCCTTCCTGTGAGCCACAGCCCCAAGATCCGGCTGCTGTCCTCCAGCCAGAGGGAGCGTCAAGCCCTTCAGGACCTTGTGGACCTGGCAGTGGAAGGGCTGAGCTCTAGCCCACAGCCCGGGAGTCGGGGCGTACCCACAGGGTTGGACCTGGTACCTAGCAGCCTTCCGCTGACGGGGTTTGTCCTTCCATGTAAGAAGACCCTGAAGAAGGATGACAGTGCCTCACTCTCTCTTGGGTTATTGGTGACAGACTTTGGGGCCATGGTCAATAATCCACACCTGAGTGATGTCCAGTTCCAGCTTGACAGTGGAGAGGTGCTTTATGCACACAAGTTTGTTCTTTATGCGAGATGCCCACTTCTTATTCAGTATGTAAGCACTGAAAGCTTTTCTTCTGAAGAGGACGGGGATCTGACCCAGCGTGCTCTGCTGAGTGATGTGAGCTCGGAGGCCGCCCATGCATTTCTGAACTACCTCTACATGGCAGACACTGACATGCCTCCCAGCCTGGTTCCGGATCTGCGTTCCCTGGCCCTAAGGTTTGGTGTGAGTGACCTTGTTCAGCTGTGTGAACAAGTGCCCGCCGTGGTGGACTTAGAGGGAGAACAGCCAGAAGAGACCAGTGAGGACTGTGAAAGCCGAGCAGAGACTTTCCTGGAACTCCTGAGGTCGGTGTGGGTAGACAATGAGGAGGAAGTGGAGACTCTGCTGAAACCTGAGCTCTGTGAAGAAGAGAGAGAAAGAGTGAATGAAGCAGAAATGGAAGAGATATATGAATTTGCTGCTACGCAGAGGAAGCTTCTCCAGTGGGGGAGAGCAGCAGACCCAGATGGGAGCACTAACCCGCATGGGGAAGACGGTGCAGTTTCTGAGCCTTCCCTGGCAGGTGTCCAGAGTAACAGACAGCTAGAAAATACAGAGCACATGGAGTCATCTGGGTTAGAAAAAGAAGAGGCACTAGCCAGCTGGGAACAAGAAGGACATTCCACTCCACTGCAGGACCAGTGCCCAGACTGGGCAGGGAAAGCAGAGGCCCAGGATGCATTGGGGGAGGCAACTGACGACCCCAGCTTCTGCAGCCGCCACAGGAGGGGGAAAGAGTGCTTGCCTTTGCACCCAAACAAGGCCCATGGCTGCAAACAGCCCCTTCCATCAAATCCAAGAGTGTCATCTGAACTGTCACAAATAACAGTTGATCATGAAGAGCAGAGTGACCATGTCAGGGAGACGCAGGCAGACATGGCCCAGGCACCTACTCCACACTCCTGCAGCCTTGTGTCACAGTCTTCTGTGGATGGAAGTCCCAGCCAGTCATGGCTTCACCTATACCACACTAGTCATTTGTCCCCCTCAGTGTCCCAGTCACACAGTAGCATTTCCAGGGTGGCATCCCCAAGATCACTGTCTCCAACTACACCAACTAAGCAGAGGAGGGGCAGTAACATTGTCACATTACGGAAAGATGCTGGCCACCACAGAGGCCAACAAAGTAGCCCCATAGCGGGACACAGAAATAGGGGCATCCTGATTTCCCCAGCAAAGTCTCCACCCATTGACCTCACACAGTCAGTACCCGAGCCCCTGAGCCCCAGGGCCCAGGATCCTCTGCATTTCGTGAAAAAGGAGGATGAGGTCATCCTGTTACTGGACTCAGATGAGGAGCTGGAGCACACCAAGACAGAGTCAGTTTCTAAAGATTCCCCAGAAGGAAGGAAAGTTCCAGAATTTAGCCCCAGGTCCTCTGAACTGTTTTCAGTCATTGATGTTGAAGAAGATCATGAACATTTCCAAAGCCCACTGAAGAGAGAGGCCGGGCTACAGCACGGGGAGGAAGGACAGCTGGGAAACCAGTCTGCGCTGGGCTGCAGAGACATCCCCTGGCTGCTCTGCAGCCAGAAAACCAGCCTGGATGAGGACAGTGCTACAGACACCTCTTGGCTGGTGCCTGCCACTCCAGGGGTCAGCAGGAGTCGTGATTGCTCATCACAGACCCAAATCAAAAGCCTTAAGACCAGGATTCCGTCAGATGAGACTGCTCAGCAAACACCTAGACCTAACTTAGAACGCAGGACCATGCTGGAAACAGCCCAGCAGTTCTCTGTGATCATGCCACACACACAGCCTATTACTCTAGGAGCCTTTGACAGCGGAAGACAGGCCTACAGAAGCCCTTCCCATCCCTACCCCAGGCACCACAGACTCTCTTCTTCACAGCCATCATGTCCTGGCCCTGATTTTACCAGGTGGTCCCAGAAATCTTCCGCACCTAGACCATGCCTGCCAAATCTGCCTGCAGCTGATGATGTGGTAGAAGTTGGGGACAGTGATGATGAGGTGGCTTCCCATCAAGGAAACAGCAGCCCCGTGCTAGATGGTGACCCCCCAGGTCCCATGGGTGACTACTGTTGGAATGAGCCTCTCTCACCAATTCCAATTGACCACTTGAATCTGGAGCGGACCGGCCCCCTGACCACAAGCAGTCCCAGCAGTCAGGTCCTGGAGGCTTTGCACAGTGATGACTGCCACTCCCCGGGACTTGGTACCACCCCCATCCGAGGAAGCTGTGGTACGCTGAGAGAGTCTCAGGAACGGTCCTCACTGGCTGGCTCTCCAGAGGCTCTGTGGGACGACTGGAATGAGGAGGAAGGACAGTCTCCAGAGGCCCCACCTGTGGCTCAAATGCTGAGTACCAGAACTCGGAAGCCAGACAGGCCAGAGACACCAAAAGGTGCTAACCAGAAAAAGAACTTGCCTCCCAAAGTGCCTATAACTCCAATGCCAAGGTATTCGATCATGGAGACCCCGGTGCTGAAGAAAGAACTGGACAGGTTTGGAGTCCGTGCTCTGCCCAAACGCCAGATGGTTCTAAAACTGAAGGAGATTTTCCAGTACACTCACCAGACTCTGGAGTCGGACTCTGAGGATGAGGTCCAGTCCCCCCAGATACCCGCAGAACTACCTTGTCGCCAGGCATCTACCACTGAGACCTGCAACCCTTCAAGGTTGCCAACTGGGGAGCCTTCACATCCTGATGGTGACGCCCAGCTCCCAGCCTCCCAGGAATCCATGGCTACCTCTGTGGACGGCAGCGACAACTCCTTTAGCTCTAAAAGCTCCTCTGCTGAGTTTGGAGCTGCGTTTGAGTATTCAGATGAAGACAAGGATGAGGAGGTCGGGGTCACTGCATCACAGGCCGCAATCCAGGCCGCAGACACAGAGGAGGCAGTGAGGCGCTATATCCGCTCTAAGCCTGCCCTGCACAGGCAGGTCCTGAGGTACCAGCCTGTGGAGCTGGCCGAGCTGCAGGCTGAGCTGAAACAGAATGGCATCCCTGTGGCCATGGGCAAGCTGTCGGACATCCTGGACGCTCAGTGCATCACCTTCACCACTGCTGCAGCCCGAAAGGAGAAACTCAAACACAAGAGACGGCAGCCCTCAGGCCGAAAGAAAAAGGATCAGAAATGA

Amino Acid Sequence

MVPESAPNGNSQPLPSCFTTTGVPSPSKPRVSELVLQRMKQFKRADPERLRHASEESPQKTALGDDVPRSPPEETVGENEYKLDATDSDAAMALALQQEFRREEASSHHDSLEEKGLFFCQMCQKNLSAMNVTRREQHVNRCLDEAEKAQRPASPRIPDCPICGKPFLTTKSRISHLKQCAVRMEVGPQLLLQAVRLQTAQPEVDGSPQVPSFSNNVGGLKRKGVTTKREPRRRKVNKPEAPSEDLLVAMALSRSEVEHCPVVPPLRLENAFSEKIRLGAEKKSRKKRPPVCPPQLVTQDSETTGRQIEDRVAQLLSEEAELSCTPPLLASKISKEELEPAGWRARLPEGKRNFAAELAALTGAWAEESFYTVGLFPPIVSQCPSKEPQLPLELPKQGEPSPRRPPASQSSLPVSHSPKIRLLSSSQRERQALQDLVDLAVEGLSSSPQPGSRGVPTGLDLVPSSLPLTGFVLPCKKTLKKDDSASLSLGLLVTDFGAMVNNPHLSDVQFQLDSGEVLYAHKFVLYARCPLLIQYVSTESFSSEEDGDLTQRALLSDVSSEAAHAFLNYLYMADTDMPPSLVPDLRSLALRFGVSDLVQLCEQVPAVVDLEGEQPEETSEDCESRAETFLELLRSVWVDNEEEVETLLKPELCEEERERVNEAEMEEIYEFAATQRKLLQWGRAADPDGSTNPHGEDGAVSEPSLAGVQSNRQLENTEHMESSGLEKEEALASWEQEGHSTPLQDQCPDWAGKAEAQDALGEATDDPSFCSRHRRGKECLPLHPNKAHGCKQPLPSNPRVSSELSQITVDHEEQSDHVRETQADMAQAPTPHSCSLVSQSSVDGSPSQSWLHLYHTSHLSPSVSQSHSSISRVASPRSLSPTTPTKQRRGSNIVTLRKDAGHHRGQQSSPIAGHRNRGILISPAKSPPIDLTQSVPEPLSPRAQDPLHFVKKEDEVILLLDSDEELEHTKTESVSKDSPEGRKVPEFSPRSSELFSVIDVEEDHEHFQSPLKREAGLQHGEEGQLGNQSALGCRDIPWLLCSQKTSLDEDSATDTSWLVPATPGVSRSRDCSSQTQIKSLKTRIPSDETAQQTPRPNLERRTMLETAQQFSVIMPHTQPITLGAFDSGRQAYRSPSHPYPRHHRLSSSQPSCPGPDFTRWSQKSSAPRPCLPNLPAADDVVEVGDSDDEVASHQGNSSPVLDGDPPGPMGDYCWNEPLSPIPIDHLNLERTGPLTTSSPSSQVLEALHSDDCHSPGLGTTPIRGSCGTLRESQERSSLAGSPEALWDDWNEEEGQSPEAPPVAQMLSTRTRKPDRPETPKGANQKKNLPPKVPITPMPRYSIMETPVLKKELDRFGVRALPKRQMVLKLKEIFQYTHQTLESDSEDEVQSPQIPAELPCRQASTTETCNPSRLPTGEPSHPDGDAQLPASQESMATSVDGSDNSFSSKSSSAEFGAAFEYSDEDKDEEVGVTASQAAIQAADTEEAVRRYIRSKPALHRQVLRYQPVELAELQAELKQNGIPVAMGKLSDILDAQCITFTTAAARKEKLKHKRRQPSGRKKKDQK*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU42471
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: All BTBD12 (SLX4) plasmids MUST be grown at 30 degrees C or less to prevent recombination.