cDNA Clone SLX4 (mouse)

• Synonyms: AI256635, AI426760, Btbd12, D16Bwg1016e
• Plasmid: pBABE Neo SLX4m C1536R
• Parent Plasmid: pBabeD neo
• Expressed: SLX4m C1536R
• Genbank: NM_177472.4
• Species: Mouse

Sequence of Insert

AGATCTATGGTTCCAGAGAGTGCTCCCAATGGCAACTCCCAGCCTCTTCCTTCCTGTTTCACGACAACTGGTGTACCAAGTCCCTCCAAACCCAGAGTCTCAGAGCTGGTTCTGCAACGGATGAAGCAGTTCAAGAGAGCAGACCCTGAGCGCCTGAGACACGCCTCAGAAGAATCCCCCCAAAAGACTGCACTGGGAGACGATGTCCCAAGGAGCCCTCCAGAGGAGACAGTGGGAGAAAATGAGTATAAACTTGATGCCACAGACAGTGATGCTGCAATGGCCCTGGCCTTACAGCAGGAGTTTAGAAGGGAGGAAGCATCCTCACACCATGACAGCCTGGAGGAGAAGGGGTTGTTCTTCTGTCAAATGTGTCAGAAAAACCTCTCAGCAATGAATGTAACCCGGAGGGAGCAACATGTGAACAGATGCCTAGATGAGGCAGAGAAGGCACAGAGACCTGCTTCACCTCGGATCCCTGATTGCCCAATATGTGGGAAACCGTTCCTCACCACCAAGAGCCGAATCAGTCACTTGAAGCAGTGTGCGGTGAGGATGGAAGTGGGTCCCCAACTCCTGCTTCAGGCTGTGAGGCTACAGACAGCTCAGCCAGAGGTGGATGGCAGCCCTCAGGTACCCAGTTTCAGCAACAACGTTGGAGGTTTGAAACGGAAAGGAGTCACCACCAAAAGGGAGCCAAGGAGGAGGAAAGTCAATAAGCCTGAGGCTCCGTCTGAAGACCTGTTGGTGGCCATGGCTCTGTCTCGCTCTGAGGTGGAGCACTGTCCAGTTGTGCCCCCGCTCAGACTGGAAAATGCTTTTTCTGAGAAGATAAGGCTAGGAGCAGAGAAGAAAAGTCGCAAGAAGAGACCCCCGGTGTGTCCCCCACAGTTGGTAACCCAAGACTCTGAGACCACAGGTAGACAGATTGAAGATCGTGTTGCCCAGCTTCTGTCAGAGGAAGCAGAACTATCTTGCACCCCACCACTTCTTGCCAGCAAGATTTCTAAGGAAGAATTGGAACCTGCAGGCTGGCGAGCACGGCTGCCTGAAGGGAAGCGGAACTTTCTGTGGGAACTCAGCGCTCTCACAGGAGCCTGGGCTGAGGAGTCCTTCTACACAGTGGGACTGTTTCCTCCAATTGTGTCCCAATGCCCTTCCAAGGAACCCCAGCTACCATTGGAGCTGCCCAAGCAAGGAGAGCCGAGTCCACGGCGGCCACCTGCTTCCCAGAGCAGCCTTCCTGTGAGCCACAGCCCCAAGATCCGGCTGCTGTCCTCCAGCCAGAGGGAGCGTCAAGCCCTTCAGGACCTTGTGGACCTGGCAGTGGAAGGGCTGAGCTCTAGCCCACAGCCCGGGAGTCGGGGCGTACCCACAGGGTTGGACCTGGTACCTAGCAGCCTTCCGCTGACGGGGTTTGTCCTTCCATGTAAGAAGACCCTGAAGAAGGATGACAGTGCCTCACTCTCTCTTGGGTTATTGGTGACAGACTTTGGGGCCATGGTCAATAATCCACACCTGAGTGATGTCCAGTTCCAGCTTGACAGTGGAGAGGTGCTTTATGCACACAAGTTTGTTCTTTATGCGAGATGCCCACTTCTTATTCAGTATGTAAGCACTGAAAGCTTTTCTTCTGAAGAGGACGGGGATCTGACCCAGCGTGCTCTGCTGAGTGATGTGAGCTCGGAGGCCGCCCATGCATTTCTGAACTACCTCTACATGGCAGACACTGACATGCCTCCCAGCCTGGTTCCGGATCTGCGTTCCCTGGCCCTAAGGTTTGGTGTGAGTGACCTTGTTCAGCTGTGTGAACAAGTGCCCGCCGTGGTGGACTTAGAGGGAGAACAGCCAGAAGAGACCAGTGAGGACTGTGAAAGCCGAGCAGAGACTTTCCTGGAACTCCTGAGGTCGGTGTGGGTAGACAATGAGGAGGAAGTGGAGACTCTGCTGAAACCTGAGCTCTGTGAAGAAGAGAGAGAAAGAGTGAATGAAGCAGAAATGGAAGAGATATATGAATTTGCTGCTACGCAGAGGAAGCTTCTCCAGTGGGGGAGAGCAGCAGACCCAGATGGGAGCACTAACCCGCATGGGGAAGACGGTGCAGTTTCTGAGCCTTCCCTGGCAGGTGTCCAGAGTAACAGACAGCTAGAAAATACAGAGCACATGGAGTCATCTGGGTTAGAAAAAGAAGAGGCACTAGCCAGCTGGGAACAAGAAGGACATTCCACTCCACTGCAGGACCAGTGCCCAGACTGGGCAGGGAAAGCAGAGGCCCAGGATGCATTGGGGGAGGCAACTGACGACCCCAGCTTCTGCAGCCGCCACAGGAGGGGGAAAGAGTGCTTGCCTTTGCACCCAAACAAGGCCCATGGCTGCAAACAGCCCCTTCCATCAAATCCAAGAGTGTCATCTGAACTGTCACAAATAACAGTTGATCATGAAGAGCAGAGTGACCATGTCAGGGAGACGCAGGCAGACATGGCCCAGGCACCTACTCCACACTCCTGCAGCCTTGTGTCACAGTCTTCTGTGGATGGAAGTCCCAGCCAGTCATGGCTTCACCTATACCACACTAGTCATTTGTCCCCCTCAGTGTCCCAGTCACACAGTAGCATTTCCAGGGTGGCATCCCCAAGATCACTGTCTCCAACTACACCAACTAAGCAGAGGAGGGGCAGTAACATTGTCACATTACGGAAAGATGCTGGCCACCACAGAGGCCAACAAAGTAGCCCCATAGCGGGACACAGAAATAGGGGCATCCTGATTTCCCCAGCAAAGTCTCCACCCATTGACCTCACACAGTCAGTACCCGAGCCCCTGAGCCCCAGGGCCCAGGATCCTCTGCATTTCGTGAAAAAGGAGGATGAGGTCATCCTGTTACTGGACTCAGATGAGGAGCTGGAGCACACCAAGACAGAGTCAGTTTCTAAAGATTCCCCAGAAGGAAGGAAAGTTCCAGAATTTAGCCCCAGGTCCTCTGAACTGTTTTCAGTCATTGATGTTGAAGAAGATCATGAACATTTCCAAAGCCCACTGAAGAGAGAGGCCGGGCTACAGCACGGGGAGGAAGGACAGCTGGGAAACCAGTCTGCGCTGGGCTGCAGAGACATCCCCTGGCTGCTCTGCAGCCAGAAAACCAGCCTGGATGAGGACAGTGCTACAGACACCTCTTGGCTGGTGCCTGCCACTCCAGGGGTCAGCAGGAGTCGTGATTGCTCATCACAGACCCAAATCAAAAGCCTTAAGACCAGGATTCCGTCAGATGAGACTGCTCAGCAAACACCTAGACCTAACTTAGAACGCAGGACCATGCTGGAAACAGCCCAGCAGTTCTCTGTGATCATGCCACACACACAGCCTATTACTCTAGGAGCCTTTGACAGCGGAAGACAGGCCTACAGAAGCCCTTCCCATCCCTACCCCAGGCACCACAGACTCTCTTCTTCACAGCCATCATGTCCTGGCCCTGATTTTACCAGGTGGTCCCAGAAATCTTCCGCACCTAGACCATGCCTGCCAAATCTGCCTGCAGCTGATGATGTGGTAGAAGTTGGGGACAGTGATGATGAGGTGGCTTCCCATCAAGGAAACAGCAGCCCCGTGCTAGATGGTGACCCCCCAGGTCCCATGGGTGACTACTGTTGGAATGAGCCTCTTTCACCAATTCCAATTGACCACTTGAATCTGGAGCGGACCGGCCCCCTGACCACAAGCAGTCCCAGCAGTCAGGTCCTGGAGGCTTTGCACAGTGATGACTGCCACTCCCCGGGACTTGGTACCACCCCCATCCGAGGAAGCTGTGGTACGCTGAGAGAGTCTCAGGAACGGTCCTCACTGGCTGGCTCTCCAGAGGCTCTGTGGGACGACTGGAATGAGGAGGAAGGACAGTCTCCAGAGGCCCCACCTGTGGCTCAAATGCTGAGTACCAGAACTCGGAAGCCAGACAGGCCAGAGACACCAAAAGGTGCTAACCAGAAAAAGAACTTGCCTCCCAAAGTGCCTATAACTCCAATGCCAAGGTATTCGATCATGGAGACCCCGGTGCTGAAGAAAGAACTGGACAGGTTTGGAGTCCGTGCTCTGCCCAAACGCCAGATGGTTCTAAAACTGAAGGAGATTTTCCAGTACACTCACCAGACTCTGGAGTCGGACTCTGAGGATGAGGTCCAGTCCCCCCAGATACCCGCAGAACTACCTTGTCGCCAGGCATCTACCACTGAGACCTGCAACCCTTCAAGGTTGCCAACTGGGGAGCCTTCACATCCTGATGGTGACGCCCAGCTCCCAGCCTCCCAGGAATCCATGGCTACCTCTGTGGACGGCAGCGACAACTCCTTTAGCTCTAAAAGCTCCTCTGCTGAGTTTGGAGCTGCGTTTGAGTATTCAGATGAAGACAAGGATGAGGAGGTCGGGGTCACTGCATCACAGGCCGCAATCCAGGCCGCAGACACAGAGGAGGCAGTGAGGCGCTATATCCGCTCTAAGCCTGCCCTGCACAGGCAGGTCCTGAGGTACCAGCCTGTGGAGCTGGCCGAGCTGCAGGCTGAGCTGAAACAGAATGGCATCCCTGTGGCCATGGGCAAGCTGTCGGACATCCTGGACGCTCAGCGCATCACCTTCACCACTGCTGCAGCCCGAAAGGAGAAACTCAAACACAAGAGACGGCAGCCCTCAGGCCGAAAGAAAAAGGATCAGAAATGAGCGGCCGC

Amino Acid Sequence

MVPESAPNGNSQPLPSCFTTTGVPSPSKPRVSELVLQRMKQFKRADPERL
RHASEESPQKTALGDDVPRSPPEETVGENEYKLDATDSDAAMALALQQEF
RREEASSHHDSLEEKGLFFCQMCQKNLSAMNVTRREQHVNRCLDEAEKAQ
RPASPRIPDCPICGKPFLTTKSRISHLKQCAVRMEVGPQLLLQAVRLQTA
QPEVDGSPQVPSFSNNVGGLKRKGVTTKREPRRRKVNKPEAPSEDLLVAM
ALSRSEVEHCPVVPPLRLENAFSEKIRLGAEKKSRKKRPPVCPPQLVTQD
SETTGRQIEDRVAQLLSEEAELSCTPPLLASKISKEELEPAGWRARLPEG
KRNFLWELSALTGAWAEESFYTVGLFPPIVSQCPSKEPQLPLELPKQGEP
SPRRPPASQSSLPVSHSPKIRLLSSSQRERQALQDLVDLAVEGLSSSPQP
GSRGVPTGLDLVPSSLPLTGFVLPCKKTLKKDDSASLSLGLLVTDFGAMV
NNPHLSDVQFQLDSGEVLYAHKFVLYARCPLLIQYVSTESFSSEEDGDLT
QRALLSDVSSEAAHAFLNYLYMADTDMPPSLVPDLRSLALRFGVSDLVQL
CEQVPAVVDLEGEQPEETSEDCESRAETFLELLRSVWVDNEEEVETLLKP
ELCEEERERVNEAEMEEIYEFAATQRKLLQWGRAADPDGSTNPHGEDGAV
SEPSLAGVQSNRQLENTEHMESSGLEKEEALASWEQEGHSTPLQDQCPDW
AGKAEAQDALGEATDDPSFCSRHRRGKECLPLHPNKAHGCKQPLPSNPRV
SSELSQITVDHEEQSDHVRETQADMAQAPTPHSCSLVSQSSVDGSPSQSW
LHLYHTSHLSPSVSQSHSSISRVASPRSLSPTTPTKQRRGSNIVTLRKDA
GHHRGQQSSPIAGHRNRGILISPAKSPPIDLTQSVPEPLSPRAQDPLHFV
KKEDEVILLLDSDEELEHTKTESVSKDSPEGRKVPEFSPRSSELFSVIDV
EEDHEHFQSPLKREAGLQHGEEGQLGNQSALGCRDIPWLLCSQKTSLDED
SATDTSWLVPATPGVSRSRDCSSQTQIKSLKTRIPSDETAQQTPRPNLER
RTMLETAQQFSVIMPHTQPITLGAFDSGRQAYRSPSHPYPRHHRLSSSQP
SCPGPDFTRWSQKSSAPRPCLPNLPAADDVVEVGDSDDEVASHQGNSSPV
LDGDPPGPMGDYCWNEPLSPIPIDHLNLERTGPLTTSSPSSQVLEALHSD
DCHSPGLGTTPIRGSCGTLRESQERSSLAGSPEALWDDWNEEEGQSPEAP
PVAQMLSTRTRKPDRPETPKGANQKKNLPPKVPITPMPRYSIMETPVLKK
ELDRFGVRALPKRQMVLKLKEIFQYTHQTLESDSEDEVQSPQIPAELPCR
QASTTETCNPSRLPTGEPSHPDGDAQLPASQESMATSVDGSDNSFSSKSS
SAEFGAAFEYSDEDKDEEVGVTASQAAIQAADTEEAVRRYIRSKPALHRQ
VLRYQPVELAELQAELKQNGIPVAMGKLSDILDAQRITFTTAAARKEKLK
HKRRQPSGRKKKDQK*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU44388
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: C1536R.; All BTBD12 (SLX4) plasmids MUST be grown at 30 degrees C or less to prevent recombination.